Patents by Inventor Lolita Zaliauskiene
Lolita Zaliauskiene has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230272394Abstract: Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule in vitro or in vivo using an RNA-guided DNA endonuclease comprising RNA sequences and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro and using a cassette containing a single repeat-spacer-repeat unit.Type: ApplicationFiled: December 13, 2022Publication date: August 31, 2023Applicant: VILNIUS UNIVERSITYInventors: Virginijus SIKSNYS, Giedrius GASIUNAS, Tautvydas KARVELIS, Arvydas LUBYS, Lolita ZALIAUSKIENE, Monika GASIUNIENE, Anja SMITH
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Patent number: 11555187Abstract: Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule in vitro or in vivo using an RNA-guided DNA endonuclease comprising RNA sequences and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro and using a cassette containing a single repeat-spacer-repeat unit.Type: GrantFiled: December 7, 2017Date of Patent: January 17, 2023Assignee: Vilnius UniversityInventors: Virginijus {hacek over (S)}ik{hacek over (s)}nys, Giedrius Gasiunas, Tautvydas Karvelis, Arvydas Lubys, Lolita Zaliauskiene, Monika Gasiuniene, Anja Smith
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Publication number: 20220267525Abstract: Disclosed herein are poly(hydroxylalkyleneimine disulfide)polymers, which have both desirable transfection properties and reduced toxicity.Type: ApplicationFiled: March 1, 2022Publication date: August 25, 2022Inventors: Sarunas ZIGMANTAS, Lolita ZALIAUSKIENE, Ricardas MAKUSKA, Alma BOCKUVIENE, Ausvydas VAREIKIS
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Patent number: 11286345Abstract: Disclosed herein are poly(hydroxylalkyleneimine disulfide)polymers, which have both desirable transfection properties and reduced toxicity.Type: GrantFiled: March 10, 2017Date of Patent: March 29, 2022Assignee: Thermo Fisher Scientific Baltics UABInventors: Sarunas Zigmantas, Lolita Zaliauskiene, Ricardas Makuska, Alma Bockuviene, Ausvydas Vareikis
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Publication number: 20190048139Abstract: Disclosed herein are poly(hydroxylalkyleneimine disulfide)polymers, which have both desirable transfection properties and reduced toxicity.Type: ApplicationFiled: March 10, 2017Publication date: February 14, 2019Inventors: Sarunas ZIGMANTAS, Lolita ZALIAUSKIENE, Ricardas MAKUSKA, Alma BOCKUVIENE, Ausvydas VAREIKIS
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Publication number: 20180187195Abstract: Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule in vitro or in vivo using an RNA-guided DNA endonuclease comprising RNA sequences and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro and using a cassette containing a single repeat-spacer-repeat unit.Type: ApplicationFiled: December 7, 2017Publication date: July 5, 2018Applicant: VILNIUS UNIVERSITYInventors: Virginijus SIKSNYS, Giedrius GASIUNAS, Tautvydas KARVELIS, Arvydas LUBYS, Lolita ZALIAUSKIENE, Monika GLEMZAITE, Anja SMITH
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Patent number: 9856456Abstract: A composition comprising a polycationic agent and a polyanionic agent, and kits comprising the composition, is provided. In embodiments, the polyanionic agent is a nucleic acid and the polycationic agent is a modified polyalkyleneimine polymer.Type: GrantFiled: September 24, 2015Date of Patent: January 2, 2018Assignee: THERMO FISHER SCIENTIFIC BALTICS UABInventors: Arunas Lagunavicius, Lolita Zaliauskiene
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Publication number: 20170152487Abstract: A composition comprising a polycationic agent and a polyanionic agent, and kits comprising the composition, is provided. In embodiments, the polyanionic agent is a nucleic acid and the polycationic agent is a modified polyalkyleneimine polymer.Type: ApplicationFiled: September 24, 2015Publication date: June 1, 2017Applicant: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Lolita Zaliauskiene
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Patent number: 9481862Abstract: A composition and method for delivering a peptide or protein into a cell comprising a polycationic agent and a polyanionic agent, wherein the polyanionic agent comprises an inorganic polyphosphate or a polyoxometalate and/or the polycationic agent comprises a polyalkyleneimine.Type: GrantFiled: January 3, 2014Date of Patent: November 1, 2016Assignee: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Lolita Zaliauskiene
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Publication number: 20160024475Abstract: A composition comprising a polycationic agent and a polyanionic agent, and kits comprising the composition, is provided. In embodiments, the polyanionic agent is a nucleic acid and the polycationic agent is a modified polyalkyleneimine polymer.Type: ApplicationFiled: September 24, 2015Publication date: January 28, 2016Applicant: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Lolita Zaliauskiene
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Patent number: 9102796Abstract: The present invention relates to a polyalkyleneimine polymer having a repeat unit comprising a hydrophilic group, agents containing the same and methods for their use.Type: GrantFiled: December 12, 2008Date of Patent: August 11, 2015Assignee: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Sarunas Zigmantas, Laurynas Riauba, Lolita Zaliauskiene, Richard Makuska, Ausvydas Vareikis, Ula Bernadisiute
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Publication number: 20150050699Abstract: Isolation or in vitro assembly of the Cas9-crRNA complex of the Streptococcus thermophilus CRISPR3/Cas system and use for cleavage of DNA bearing a nucleotide sequence complementary to the crRNA and a proto-spacer adjacent motif. Methods for site-specific modification of a target DNA molecule in vitro or in vivo using an RNA-guided DNA endonuclease comprising RNA sequences and at least one of an RuvC active site motif and an HNH active site motif; for conversion of Cas9 polypeptide into a nickase cleaving one strand of double-stranded DNA by inactivating one of the active sites (RuvC or HNH) in the polypeptide by at least one point mutation; for assembly of active polypeptide-polyribonucleotides complex in vivo or in vitro; and for re-programming a Cas9-crRNA complex specificity in vitro and using a cassette containing a single repeat-spacer-repeat unit.Type: ApplicationFiled: March 20, 2013Publication date: February 19, 2015Inventors: Virginijus Siksnys, Giedrius Gasiunas, Tautvydad Karvelis, Arvydas Lubys, Lolita Zaliauskiene, Monika Glemzaite, Anja Smith
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Patent number: 8951957Abstract: A composition and method comprising a polycationic agent and a polyanionic agent for delivering a peptide or protein into a cell.Type: GrantFiled: October 11, 2010Date of Patent: February 10, 2015Assignee: Fermentas UABInventors: Arunas Lagunavicius, Lolita Zaliauskiene
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Publication number: 20140120618Abstract: A composition and method for delivering a peptide or protein into a cell.Type: ApplicationFiled: January 3, 2014Publication date: May 1, 2014Applicant: Thermo Fisher Scientific Baltics UABInventors: Arunas Lagunavicius, Lolita Zaliauskiene
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Publication number: 20110086794Abstract: A composition and method for delivering a peptide or protein into a cell.Type: ApplicationFiled: October 11, 2010Publication date: April 14, 2011Applicant: FERMENTAS UABInventors: Arunas Lagunavicius, Lolita Zaliauskiene
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Publication number: 20100041739Abstract: The present invention relates to a polyalkyleneimine polymer having a repeat unit comprising a hydrophilic group, agents containing the same and methods for their use.Type: ApplicationFiled: December 12, 2008Publication date: February 18, 2010Applicant: FERMENTAS UABInventors: Arunas Lagunavicius, Sarunas Zigmantas, Laurynas Riauba, Lolita Zaliauskiene, Richard Makuska, Ausvydas Vareikis, Ula Bernadisiute