Abstract: Disclosed herein is a multi-channel line microscope for imaging a sample by moving a microscope stage across two or more reflected excitation lines positioned relative to each other so that each excitation line excites a spatially distinct horizontal line in the image plane of the sample. Also disclosed herein are methods to image a sample by moving a microscope stage across two or more reflected excitation lines positioned relative to each other so that each excitation line excites a spatially distinct horizontal line in the image plane of the sample. Also disclosed herein is an apparatus and methods of use of the apparatus for a broad range of applications.

Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci.

Abstract: Disclosed herein are methods and systems for detecting and/or quantifying cellular targets such as nucleic acids in cells, tissues, organs or organisms. Through sequential barcoding, it is possible to perform high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci. In some embodiments, error correction is implemented through use of barcodes that can tolerate mistakes and missing data during sequential hybridization of probes to selected targets.

Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. In some embodiments, through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci.

Abstract: Methods and systems are provided for creating molecular barcodes or indicia for cellular constituents within single cells and for resolving such barcodes or indicia with super-resolution technologies such as super-resolution microscopy. By this approach, numerous molecular species that can be measured simultaneously in single cells. It has been demonstrated that multiple mRNA transcripts can be labeled with a spatially ordered sequence of fluorophores, and that barcodes can be resolved. In addition, alternative splicing events can be characterized by identifying and quantifying mRNA isoforms in an individual cell.

Abstract: Methods and systems are provided for creating molecular barcodes or indicia for cellular constituents within single cells and for resolving such barcodes or indicia with super resolution technologies such as super resolution microscopy. By this approach, numerous molecular species that can be measured simultaneously in single cells. It has been demonstrated that multiple mRNA transcripts can be labeled with a spatially ordered sequence of fluorophores, and that barcode can be resolved. The methods and systems can be used for genome-wide transcriptional profiling in individual cells by super-resolution barcoding and suggest a general strategy to bring large-scale-omics approach into single cells.

Abstract: A zoom lens including a first lens group, a second lens group, and a third lens group is provided. The first lens group is disposed between an object side and an image side and has at least one aspheric surface. The second lens group has a positive refractive power and is disposed between the first lens group and the image side. The third lens group has a positive refractive power and is disposed between the second lens group and the image side. The third lens group has at least one aspheric surface. The third lens group is suitable to move relative to the second lens group for focusing.

Abstract: Methods and systems for recording and mapping lineage information and molecular events in individual cells are provided. Molecular changes, which may result from random or specific molecular events, are introduced to defined regions in cells over multiple cell cycle generations. Techniques such as fluorescent imaging are applied to track and identify the molecular changes before such information is used for lineage analysis or for identifying key processes and key players in cellular pathways.

Abstract: The present disclosure relates to methods involving HCR reactions that involve using trigger oligos to activate probes that initiate HCR.

Abstract: An exemplary atomizing core includes a cylindrical main body and an atomizing rod. The cylindrical main body has a central axis. The cylindrical main body extends from the main body along the central axis. The main body and the atomizing rod are both made of porous bodies. The main body defines a through hole along the central axis.

Abstract: A liquid stopper for an electronic cigarette, includes a main body configured for stopping a tobacco liquid of the electronic cigarette. The main body includes a bottom surface, at least one wire hole in communication with the bottom surface, and at least one wire groove formed in the bottom surface and in communication with the at least one wire hole. The at least one wire hole is configured to receive at least one conductive wire of the electronic cigarette, and the at least one wire groove is configured to guide the at least one conductive wire to a peripheral wall of the main body. An electronic cigarette using the liquid stopper is also provided.

Abstract: An exemplary atomizer includes a liquid supply component, an atomizing assembly, and a pricking means. The liquid supply component is configured for storing tobacco liquid. The liquid supply component has a sealing means for sealing the tobacco liquid therein. The pricking means for pricking the sealing structure so that the tobacco liquid flows to the atomizing assembly. The atomizing assembly is configured for heating the tobacco liquid to form aerosol.

Abstract: A wide angle lens includes, in order from an object side to an image side along an optical axis, a negative first lens, a negative second lens, a positive third lens, a positive fourth lens, a negative fifth lens, and a positive sixth lens, wherein the sixth lens is adhered to the fifth lens to form a doublet lens. In addition, the wide angle lens further satisfies the condition of SD2/R2>0.6, where SD2 is half of an effective aperture of a surface of the first lens which faces the image side, and R2 is a radius of curvature of the surface of the first lens which faces the image side. Whereby, the FOV of the wide angle lens can be greater than 140 degrees, and the wide angle lens is compatible with a large-sized, e.g., 1/2.3?, photosensitive component.

Abstract: Disclosed herein are methods and systems for detecting and/or quantifying cellular targets such as nucleic acids in cells, tissues, organs or organisms. Through sequential barcoding, it is possible to perform high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci. In some embodiments, error correction is implemented through use of barcodes that can tolerate mistakes and missing data during sequential hybridization of probes to selected targets.

Abstract: A wide angle lens includes, in order from an object side to an image side along an optical axis, a negative first lens, a negative second lens, a positive third lens, a positive fourth lens, a negative fifth lens, and a positive sixth lens, wherein the sixth lens is adhered to the fifth lens to form a doublet lens. In addition, the wide angle lens further satisfies the condition of SD2/R2>0.6, where SD2 is half of an effective aperture of a surface of the first lens which faces the image side, and R2 is a radius of curvature of the surface of the first lens which faces the image side. Whereby, the FOV of the wide angle lens can be greater than 140 degrees, and the wide angle lens is compatible with a large-sized, e.g., 1/2.3?, photosensitive component.

Abstract: The present invention, among other things, provides technologies for detecting and/or quantifying nucleic acids in cells, tissues, organs or organisms. Through sequential barcoding, the present invention provides methods for high-throughput profiling of a large number of targets, such as transcripts and/or DNA loci. In some embodiments, nucleic acid probes include a signal moiety connected with a binding sequence via a cleavable linker.

Abstract: An exemplary atomizing core includes a cylindrical main body and an atomizing rod. The cylindrical main body has a central axis. The cylindrical main body extends from the main body along the central axis. The main body and the atomizing rod are both made of porous bodies. The main body defines a through hole along the central axis.

Abstract: Disclosed herein are methods and systems for analyzing visual data from multiple rounds of hybridization interactions where the same molecular target is detected by probes with different detectable labels. In particular, disclosed herein are methods and systems for analyzing sequential hybridization images for molecular profiling, where the images are obtained using multiplex fluorescence in situ hybridization (FISH).

Abstract: A zoom lens including a first lens group, a second lens group, and a third lens group is provided. The first lens group is disposed between an object side and an image side and has at least one aspheric surface. The second lens group has a positive refractive power and is disposed between the first lens group and the image side. The third lens group has a positive refractive power and is disposed between the second lens group and the image side. The third lens group has at least one aspheric surface. The third lens group is suitable to move relative to the second lens group for focusing.

Abstract: An exemplary atomizer includes a liquid supply component, an atomizing assembly, and a pricking means. The liquid supply component is configured for storing tobacco liquid. The liquid supply component has a sealing means for sealing the tobacco liquid therein. The pricking means for pricking the sealing structure so that the tobacco liquid flows to the atomizing assembly. The atomizing assembly is configured for heating the tobacco liquid to form aerosol.