Abstract: A preparation method of a molecularly imprinted polymer (MIP) for separation and concentration of 4-methylsterane compounds is provided. A template molecule, a functional monomer, a porogen, an initiator and a cross-linking agent are mixed and subjected to polymerization to prepare a polymer with the template molecule, which is then treated to remove the template molecule, so as to give the desired MIP. The template molecule is ?-sitosterol or deoxycholic acid. The obtained molecularly imprinted polymer has multiple stable hole structures and binding sites inside, and has memory and recognition functions for the 4-methylsterane compounds, exhibiting excellent specific adsorption performance. The MIP can contribute to the accurate identification of correlation between depositional environment and maturity of crude oils or source rocks. A molecularly imprinted polymer prepared by such method, a chromatographic column and an application thereof are also provided.

Abstract: A preparation method of a molecularly imprinted polymer (MIP) for separation and concentration of 4-methylsterane compounds is provided. A template molecule, a functional monomer, a porogen, an initiator and a cross-linking agent are mixed and subjected to polymerization to prepare a polymer with the template molecule, which is then treated to remove the template molecule, so as to give the desired MIP. The template molecule is ?-sitosterol or deoxycholic acid. The obtained molecularly imprinted polymer has multiple stable hole structures and binding sites inside, and has memory and recognition functions for the 4-methylsterane compounds, exhibiting excellent specific adsorption performance. The MIP can contribute to the accurate identification of correlation between depositional environment and maturity of crude oils or source rocks. A molecularly imprinted polymer prepared by such method, a chromatographic column and an application thereof are also provided.

Abstract: A Pseudomonas monteilii strain with salinity-alkalinity tolerance is provided. The strain is Pseudomonas monteilii 9-2, which has been deposited in China General Microbiological Culture Collection Center (CGMCC) on Oct. 25, 2021, with an accession number of CGMCC No. 23666; and an internal transcribed spacer (ITS) sequence is shown in SEQ ID NO: 1. This application further provides an application of the Pseudomonas monteilii strain in the degradation of petroleum hydrocarbons in a saline-alkali environment.

Abstract: A Pseudomonas monteilii strain with salinity-alkalinity tolerance is provided. The strain is Pseudomonas monteilii 9-2, which has been deposited in China General Microbiological Culture Collection Center (CGMCC) on Oct. 25, 2021, with an accession number of CGMCC No. 23666; and an internal transcribed spacer (ITS) sequence is shown in SEQ ID NO: 1. This application further provides an application of the Pseudomonas monteilii strain in the degradation of petroleum hydrocarbons in a saline-alkali environment.

Abstract: A method for separating diastereomers of pristane. A pristane sample is prepared, and then injected into a chromatographic instrument equipped with a chiral chromatographic column, where a stationary phase of the chiral chromatographic column has a preset pore size. The pristane diastereomers in the pristane sample are separated by the chiral chromatographic column, and the components produced by the separation of the pristane diastereomers sequentially enter a mass spectrometer for detection and analysis.

Abstract: A method for separating diastereomers of pristane. A pristane sample is prepared, and then injected into a chromatographic instrument equipped with a chiral chromatographic column, where a stationary phase of the chiral chromatographic column has a preset pore size. The pristane diastereomers in the pristane sample are separated by the chiral chromatographic column, and the components produced by the separation of the pristane diastereomers sequentially enter a mass spectrometer for detection and analysis.