Patents by Inventor Lonnie O. Ingram
Lonnie O. Ingram has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 9322043Abstract: The invention provides methods for treatment of feedstock to reduce the relative viscosity and promote release of fermentable sugars.Type: GrantFiled: March 10, 2011Date of Patent: April 26, 2016Assignee: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Lonnie O. Ingram, Claudia C. Geddes, James J. Peterson, Michael T. Mullinnix, Keelnatham Shanmugam
-
Patent number: 8900835Abstract: Genetically modified microorganisms having the ability to produce D(?)-lactic acid at temperatures between 30° C. and 55° C. are provided. In various embodiments, the microorganisms may have the chromosomal lactate dehydrogenase (ldh) gene and/or the chromosomal acetolactate synthase (alsS) gene inactivated. Exemplary microorganisms for use in the disclosed methods are Bacillus spp., such as Bacillus coagulans.Type: GrantFiled: November 22, 2011Date of Patent: December 2, 2014Assignee: University of Florida Research Foundation, Inc.Inventors: Qingzhao Wang, Keelnatham T. Shanmugam, Lonnie O. Ingram
-
Patent number: 8716002Abstract: The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed.Type: GrantFiled: August 8, 2007Date of Patent: May 6, 2014Assignee: University of Florida Research Foundation, Inc.Inventors: Lorraine P. Yomano, Sean W. York, Shengde Zhou, Keelnatham Shanmugam, Lonnie O. Ingram
-
Patent number: 8652817Abstract: Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium.Type: GrantFiled: June 30, 2006Date of Patent: February 18, 2014Assignee: Univeristy of Florida Research Foundation, Inc.Inventors: Brent E. Wood, Lonnie O. Ingram, Lorraine P. Yomano, Sean W. York
-
Patent number: 8465953Abstract: Non-recombinant bacteria that produce ethanol as the primary fermentation product, associated nucleic acids and polypeptides, methods for producing ethanol using the bacteria, and kits are disclosed.Type: GrantFiled: April 26, 2007Date of Patent: June 18, 2013Assignee: University of Florida Research Foundation, Inc.Inventors: Youngnyun Kim, Keelnatham Shanmugam, Lonnie O. Ingram
-
Publication number: 20130098356Abstract: The invention provides methods for treatment of feedstock to reduce the relative viscosity and promote release of fermentable sugars.Type: ApplicationFiled: March 10, 2011Publication date: April 25, 2013Applicant: University of Florida Research Foundation, Inc.Inventors: Lonnie O. Ingram, Claudia C. Geddes, James J. Peterson, Michael T. Mullinnix, Keelnatham Shanmugam
-
Publication number: 20120129231Abstract: Genetically modified microorganisms having the ability to produce D(?)-lactic acid at temperatures between 30° C. and 55° C. are provided. In various embodiments, the microorganisms may have the chromosomal lactate dehydrogenase (ldh) gene and/or the chromosomal acetolactate synthase (alsS) gene inactivated. Exemplary microorganisms for use in the disclosed methods are Bacillus spp., such as Bacillus coagulans.Type: ApplicationFiled: November 22, 2011Publication date: May 24, 2012Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: QINGZHAO WANG, Keelnatham T. Shanmugam, Lonnie O. Ingram
-
Publication number: 20120108855Abstract: The invention provides isolated or recombinant ethanologenic bacteria that have increased expression of transhydrogenase genes and methods of preparation. The invention also provides methods of producing ethanol using the bacterium and corresponding kits.Type: ApplicationFiled: March 15, 2010Publication date: May 3, 2012Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Lonnie O. Ingram, Elliot N. Miller, Laura R. Jarboe
-
Publication number: 20100196978Abstract: Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium.Type: ApplicationFiled: June 30, 2006Publication date: August 5, 2010Applicant: The University of Florida Research Foundation, IncInventors: Brent E. Wood, Lonnie O. Ingram, Lorraine P. Yomano, Sean W. York
-
Publication number: 20100112656Abstract: The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed.Type: ApplicationFiled: August 8, 2007Publication date: May 6, 2010Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Lorraine P. Yomano, Sean W. York, Shengde Zhou, Keelnatham Shanmugam, Lonnie O. Ingram
-
Publication number: 20090286293Abstract: Non-recombinant bacteria that produce ethanol as the primary fermentation product, associated nucleic acids and polypeptides, methods for producing ethanol using the bacteria, and kits are disclosed.Type: ApplicationFiled: April 26, 2007Publication date: November 19, 2009Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Youngnyun Kim, Keelnatham Shanmugam, Lonnie O. Ingram
-
Patent number: 7326551Abstract: The invention provides isolated nucleic acids molecules which encode pyruvate decarboxylase enzymes having improved decarboxylase activity, substrate affinity, thermostability, and activity at different pH. The nucleic acids of the invention also have a codon usage which allows for high expression in a variety of host cells. Accordingly, the invention provides recombinant expression vectors containing such nucleic acid molecules, recombinant host cells comprising the expression vectors, host cells further comprising other ethanologenic enzymes, and methods for producing useful substances, e.g., acetaldehyde and ethanol, using such host cells.Type: GrantFiled: April 30, 2002Date of Patent: February 5, 2008Assignee: University of Florida Research Foundation, Inc.Inventors: Julie A. Maupin-Furlow, Lee Ann Talarico, Krishnan Chandra Raj, Lonnie O. Ingram
-
Patent number: 7192772Abstract: Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.Type: GrantFiled: July 21, 2000Date of Patent: March 20, 2007Assignee: The University of Florida Research Foundations, Inc.Inventors: Lonnie O. Ingram, Kazuyoshi Ohta, Brent E. Wood
-
Publication number: 20040067555Abstract: The invention provides recombinant host cells containing at least one heterologous polynucleotide encoding a polysaccharase under the transcriptional control of a surrogate promoter capable of increasing the expression of the polysaccharase. In addition, the invention further provides such hosts with genes encoding secretory protein/s to facilitate the secretion of the expressed polysaccharase. Preferred hosts of the invention are ethanologenic and capable of carrying out simultaneous saccharification fermentation resulting in the production of ethanol from complex cellulose substrates.Type: ApplicationFiled: February 27, 2003Publication date: April 8, 2004Applicant: University of Florida Research Foundation, IncorporatedInventors: Lonnie O. Ingram, Shengde Zhou
-
Publication number: 20030087368Abstract: The invention provides isolated nucleic acids molecules which encode pyruvate decarboxylase enzymes having improved decarboxylase activity, substrate affinity, thermostability, and activity at different pH. The nucleic acids of the invention also have a codon usage which allows for high expression in a variety of host cells. Accordingly, the invention provides recombinant expression vectors containing such nucleic acid molecules, recombinant host cells comprising the expression vectors, host cells further comprising other ethanologenic enzymes, and methods for producing useful substances, e.g., acetaldehyde and ethanol, using such host cells.Type: ApplicationFiled: April 30, 2002Publication date: May 8, 2003Applicant: University of FloridaInventors: Julie A. Maupin-Furlow, Lee Ann Talarico, Krishnan Chandra Raj, Lonnie O. Ingram
-
Publication number: 20030054500Abstract: This invention presents a method of improving enzymatic degradation of lignocellulose, as in the production of ethanol from lignocellulosic material, through the use of ultrasonic treatment. The invention shows that ultrasonic treatment reduces cellulase requirements by ⅓ to ½. With the cost of enzymes being a major problem in the cost-effective production of ethanol from lignocellulosic material, this invention presents a significant improvement over presently available methods.Type: ApplicationFiled: December 21, 2001Publication date: March 20, 2003Applicant: The University of Florida Research FoundationInventors: Lonnie O. Ingram, Brent E. Wood
-
Publication number: 20020160469Abstract: This invention presents a method for the production of ethanol that utilizes a soy hydrolysate-based nutrient medium or a yeast autolysate-based medium nutrient medium in conjunction with ethanologenic bacteria and a fermentable sugar for the cost-effective production of ethanol from lignocellulosic biomass. The invention offers several advantages over presently available media for use in ethanol production, including consistent quality, lack of toxins and wide availability.Type: ApplicationFiled: December 17, 2001Publication date: October 31, 2002Applicant: University of Florida Research Foundation, Inc.Inventors: Lonnie O. Ingram, Sean W. York
-
Patent number: 6333181Abstract: This invention presents a method of improving enzymatic degradation of lignocellulose, as in the production of ethanol from lignocellulosic material, through the use of ultrasonic treatment. The invention shows that ultrasonic treatment reduces cellulase requirements by ⅓ to ½. With the cost of enzymes being a major problem in the cost-effective production of ethanol from lignocellulosic material, this invention presents a significant improvement over presently available methods.Type: GrantFiled: April 7, 1997Date of Patent: December 25, 2001Assignee: University of Florida Research Foundation, Inc.Inventors: Lonnie O. Ingram, Brent E. Wood
-
Patent number: 6130076Abstract: This invention presents a method for the production of ethanol that utilizes a soy hydrolysate-based nutrient medium or a yeast autolysate-based medium nutrient medium in conjunction with ethanologenic bacteria and a fermentable sugar for the cost-effective production of ethanol from lignocellulosic biomass. The invention offers several advantages over presently available media for use in ethanol production, including consistent quality, lack of toxins and wide availability.Type: GrantFiled: June 19, 1997Date of Patent: October 10, 2000Assignee: University of Florida Research Foundation, Inc.Inventor: Lonnie O. Ingram
-
Patent number: 6107093Abstract: Recombinant host cells are obtained that comprise (A) a heterologous, polypeptide-encoding polynucleotide segment, stably integrated into a chromosome, which is under transcriptional control of an endogenous promoter and (B) a mutation that effects increased expression of the heterologous segment, resulting in enhanced production by the host cells of each polypeptide encoded by that segment, relative to production of each polypeptide by the host cells in the absence of the mutation. The increased expression thus achieved is retained in the absence of conditions that select for cells displaying such increased expression. When the integrated segment comprises, for example, ethanol-production genes from an efficient ethanol producer like Zymomonas mobilis, recombinant Escherichia coli and other enteric bacterial cells within the present invention are capable of converting a wide range of biomass-derived sugars efficiently to ethanol.Type: GrantFiled: August 14, 1998Date of Patent: August 22, 2000Assignee: University of Florida Research Foundation, Inc.Inventors: Lonnie O. Ingram, Kazuyoshi Ohta, Brent E. Wood