Abstract: Described herein are a method for transferring an embossed structure, which includes at least the steps (1-i) and (2-i) or (1-ii) and (2-ii), where steps (1-i) and (2-i) or (1-ii) and (2-ii) are carried out using an embossing tool (P1) including at least one embossing die (p1), where the embossing die (p1) of the embossing tool (P1) is pretreated, before the implementation of step (2-i) or before the implementation of step (1-ii), with at least one organic solvent and/or at least one reactive diluent, and also a method of using a corresponding pretreated embossing tool (P1) including at least one embossing die (p1) for the purpose of transferring an embossed structure in such a way.

Abstract: A tissue ablating laser device (100) comprises a laser source (110) configured to generate a base laser beam (140) and a beam shaping optics (120) configured to receive the base laser beam (140) and to transform the base laser beam (140) to an emitting laser beam (143). The beam shaping optics (120) further is configured to focus the base laser beam (140) such that the emitting laser beam (143) has a focusing angle (142) of about 10° or less.

Abstract: The present disclosure provides a non-naturally occurring CRISPR-Cas system comprising: a Cas9 effector protein capable of generating cohesive ends (stiCas9), and a guide polynucleotide that forms a complex with the stiCas9 and comprising a guide sequence, wherein the guide sequence hybridizes with a target sequence in a eukaryotic cell but does not hybridize to a sequence in a bacterial cell, and wherein the complex does not occur in nature. The present disclosure also provides a method of introducing a sequence of interest into a chromosome of a cell. Finally, the present disclosure provides for a method of modifying one or more nucleotides using seamless mutagenesis.