Abstract: The present invention relates to a method for producing a cutinase-like enzyme (CLE1) in a S. cerevisiae cell, comprising heterologously expressing a codon-optimised nucleic acid encoding the cutinase-like enzyme and operably linked to an engineered promoter in the cell. The invention further relates to recombinant S. cerevisiae cells capable of heterologously expressing the cutinase-like enzyme and to cutinase-like enzyme obtained from the cells or prepared by the methods. Also provided are methods of preparing a cell-free supernatant comprising a cutinase-like enzyme from the recombinant S. cerevisiae cells and the use thereof or of the recombinant S. cerevisiae cells in hydrolysing bioplastic polymers.

Abstract: The invention relates to the combination of the TLG1 glucoamylase from Thermomyces lanuginoses with: (i) the SFA1 alpha-amylase from Saccharomycopsis fibuligera; and/or (ii) the LKA alpha-amylase. The enzyme combinations may be expressed in a host cell (e.g. in Saccharomyces cerevisiae) or provided as an enzyme composition. Methods for making the enzyme combinations of the invention are provided. The invention also relates to a yeast strain which exhibits amylolytic activity and promising fermentative abilities. Processes for producing a fermentation product (in particular alcohol) from starch-containing material are described.

Abstract: The invention relates to the combination of the TLG1 glucoamylase from Thermomyces lanuginoses with: (i) the SFA1 alpha-amylase from Saccharomycopsis fibuligera; and/or (ii) the LKA alpha-amylase. The enzyme combinations may be expressed in a host cell (e.g. in Saccharomyces cerevisiae) or provided as an enzyme composition. Methods for making the enzyme combinations of the invention are provided. The invention also relates to a yeast strain which exhibits amylolytic activity and promising fermentative abilities. Processes for producing a fermentation product (in particular alcohol) from starch-containing material are described.