Abstract: The present invention relates to a method for detecting infections caused by or associated with siderophore-secreting microorganisms and kits and components used therein for carrying out the method. In one aspect the invention relates to a method for detecting siderophores and/or detecting infections caused by or associated with siderophore-secreting microorganisms in a biological sample of a subject, the method comprising providing a solid support having either bound siderophore or a conjugate thereof, or bound anti-siderophore antibody; reacting the bound siderophore or a conjugate thereof, with an anti-siderophore antibody, and a biological sample of a subject; or reacting the bound anti-siderophore antibody with a siderophore or conjugate thereof, and a biological sample of a subject; and detecting and/or quantifying the presence of the siderophore in the biological sample.