Patents by Inventor Lorraine P. Yomano
Lorraine P. Yomano has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 9347077Abstract: The subject invention pertains to overexpression of a putative oxidoreductase (ucpA) for increasing furfural tolerance in genetically modified microorganisms. Genetically modified microorganisms capable of overexpressing UcpA are also provided. Increased expression of ucpA was shown to increase furfural tolerance by 50%, and to permit the fermentation of sugars to products in the presence of 15 mM furfural.Type: GrantFiled: July 9, 2012Date of Patent: May 24, 2016Assignee: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATEDInventors: Xuan Wang, Elliot N. Miller, Lorraine P. Yomano, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
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Publication number: 20150299670Abstract: Four genetic traits have been identified that increase furfural tolerance in microorganisms, such as ethanol-producing Escherichia coli LY180 (strain W derivative). Increased expression of fucO, ucpA or pntAB, and deletion of yqhD were associated with the increase in furfural tolerance. Microorganisms engineered for resistance to furfural were also more resistant to the mixture of inhibitors in hemicellulose hydrolysates, confirming the importance of furfural as an inhibitory component. The combinations of genetic traits disclosed in this application can be applied, generally, to other microorganisms, such as Gram negative and Gram positive bacterial cells, yeast and fungi to increase furfural tolerance in microorganisms used to make industrially useful products.Type: ApplicationFiled: November 14, 2013Publication date: October 22, 2015Inventors: XUAN WANG, LORRAINE P. YOMANO, JAMES Y. LEE, SEAN W. YORK, HUABAO ZHENG, MICHAEL TODD MULLINNIX, KEELNATHAM T. SHANMUGAM, LONNIE O'NEAL INGRAM
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Patent number: 9157102Abstract: The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.Type: GrantFiled: March 29, 2012Date of Patent: October 13, 2015Assignee: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INCORPORATEDInventors: Elliot N. Miller, Xueli Zhang, Lorraine P. Yomano, Xuan Wang, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
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Patent number: 9150887Abstract: The invention relates to bacterium that have increased resistance to furfural and methods of preparation. The invention also relates to methods of producing ethanol using the bacterium and corresponding kits.Type: GrantFiled: January 4, 2010Date of Patent: October 6, 2015Assignee: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Elliot Norman Miller, Laura R. Jarboe, Lorraine P. Yomano, Sean W. York, Keelnatham Shanmugam, Lonnie O'Neal Ingram
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Publication number: 20140212933Abstract: The subject invention pertains to overexpression of a putative oxidoreductase (ucpA) for increasing furfural tolerance in genetically modified microorganisms. Genetically modified microorganisms capable of overexpressing UcpA are also provided. Increased expression of ucpA was shown to increase furfural tolerance by 50%, and to permit the fermentation of sugars to products in the presence of 15 mM furfural.Type: ApplicationFiled: July 9, 2012Publication date: July 31, 2014Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Xuan Wang, Elliot N. Miller, Lorraine P. Yomano, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
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Publication number: 20140141493Abstract: Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium.Type: ApplicationFiled: January 6, 2014Publication date: May 22, 2014Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Lonnie O' Neal Ingram, Lorraine P. Yomano, Sean W. York, Brent E. Wood
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Patent number: 8716002Abstract: The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed.Type: GrantFiled: August 8, 2007Date of Patent: May 6, 2014Assignee: University of Florida Research Foundation, Inc.Inventors: Lorraine P. Yomano, Sean W. York, Shengde Zhou, Keelnatham Shanmugam, Lonnie O. Ingram
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Patent number: 8652817Abstract: Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium.Type: GrantFiled: June 30, 2006Date of Patent: February 18, 2014Assignee: Univeristy of Florida Research Foundation, Inc.Inventors: Brent E. Wood, Lonnie O. Ingram, Lorraine P. Yomano, Sean W. York
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Publication number: 20140024086Abstract: The subject invention pertains to the discovery that the NADH-dependent propanediol oxidoreductase (FucO) can reduce furfural. This allows for a new approach to improve furfural tolerance in bacterial and/or yeast cells used to produce desired products. Thus, novel biocatalysts (bacterial, fungal or yeast cells) exhibiting increased tolerance to furfural and 5-hydroxymethylfurfural (5-HMF) are provided as are methods of making and using such biocatalysts for the production of a desired product.Type: ApplicationFiled: March 29, 2012Publication date: January 23, 2014Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Elliot N. Miller, Xueli Zhang, Lorraine P. Yomano, Xuan Wang, Keelnatham T. Shanmugam, Lonnie O'Neal Ingram
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Publication number: 20120077241Abstract: The invention relates to bacterium that have increased resistance to furfural and methods of preparation. The invention also relates to methods of producing ethanol using the bacterium and corresponding kits.Type: ApplicationFiled: January 4, 2010Publication date: March 29, 2012Applicant: University of Florida Research Foundation, Inc.Inventors: Elliot Norman Miller, Laura R. Jarboe, Lorraine P. Yomano, Sean W. York, Keelnatham Shanmugam, Lonnie O'Neil Ingram
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Publication number: 20100196978Abstract: Disclosed are recombinant host cells suitable for degrading an oligosaccharide that have been optimized for growth and production of high yields of ethanol, and methods of making and using these cells. The invention further provides minimal media comprising urea-like compounds for economical production of ethanol by recombinant microorganisms. Recombinant host cells in accordance with the invention are modified by gene mutation to eliminate genes responsible for the production of unwanted products other than ethanol, thereby increasing the yield of ethanol produced from the oligosaccharides, relative to unmutated parent strains. The new and improved strains of recombinant bacteria are capable of superior ethanol productivity and yield when grown under conditions suitable for fermentation in minimal growth media containing inexpensive reagents. Systems optimized for ethanol production combine a selected optimized minimal medium with a recombinant host cell optimized for use in the selected medium.Type: ApplicationFiled: June 30, 2006Publication date: August 5, 2010Applicant: The University of Florida Research Foundation, IncInventors: Brent E. Wood, Lonnie O. Ingram, Lorraine P. Yomano, Sean W. York
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Publication number: 20100112656Abstract: The invention provides recombinant bacteria, which comprise a full complement of heterologous ethanol production genes. Expression of the full complement of heterologous ethanol production genes causes the recombinant bacteria to produce ethanol as the primary fermentation product when grown in mineral salts medium, without the addition of complex nutrients. Methods for producing the recombinant bacteria and methods for producing ethanol using the recombinant bacteria are also disclosed.Type: ApplicationFiled: August 8, 2007Publication date: May 6, 2010Applicant: UNIVERSITY OF FLORIDA RESEARCH FOUNDATION, INC.Inventors: Lorraine P. Yomano, Sean W. York, Shengde Zhou, Keelnatham Shanmugam, Lonnie O. Ingram