Patents by Inventor Lothar Breitkopf

Lothar Breitkopf has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Publication number: 20220340954
    Abstract: The present invention provides a poly(alkylene oxide) polymer based size selective nucleic acid enrichment method for enriching target nucleic acid molecules from a nucleic acid containing sample which comprises target nucleic acid molecules and non-target nucleic acid molecules, wherein the target nucleic acid molecules are longer than the non-target nucleic acid molecules, the method comprising (a) preparing a binding mixture comprising—the nucleic acid containing sample, —a poly(alkylene oxide) polymer and—a salt and binding nucleic acid molecules to a solid phase which comprises a functional group, preferably carboxylated magnetic particles, wherein the bound nucleic acid molecules comprise target nucleic acid molecules; (b) preferably separating the solid phase with the bound nucleic acid molecules from the remaining sample; (c) contacting the solid phase with the bound nucleic acid molecules at least once with a reagent composition comprising a poly(alkylene oxide) polymer and a salt to selectively elut
    Type: Application
    Filed: June 26, 2020
    Publication date: October 27, 2022
    Inventors: Lothar BREITKOPF, Jasper STROEDER
  • Publication number: 20220259637
    Abstract: The present invention provides a poly(alkylene oxide) polymer based size selective method for enriching nucleic acid molecules having a length below a cut-off value from a nucleic acid containing sample, the method comprising: (a) preparing a binding mixture comprising —the nucleic acid containing sample, —a poly(alkylene oxide) polymer and —a salt and binding nucleic acid molecules of different sizes to a solid phase which comprises a functional group, preferably carboxylated magnetic particles; (b) separating the solid phase with the bound nucleic acid molecules from the remaining sample; and (c) contacting the solid phase with the bound nucleic acid molecules at least once with an elution composition comprising a poly(alkylene oxide) polymer and a salt to selectively elute nucleic acid molecules having a length below the cut-off value from the solid phase while larger nucleic acid molecules having a length above the cut-off value remain bound to the solid phase, wherein the concentration (w/v) of the poly(
    Type: Application
    Filed: June 26, 2020
    Publication date: August 18, 2022
    Inventors: Lothar BREITKOPF, Jasper STROEDER, Markus SPRENGER-HAUSSELS
  • Publication number: 20140140804
    Abstract: Magnetic rack system comprising a holder having trough-holes for receiving tubes, a base having a plurality of receptacles with at least one magnet, wherein trough-holes in the holder are arranged relative to the base such that each tube can be positioned in a respective receptacle in a pre-determined position relative to the magnet, and an adapter, wherein the adapter is designed to allow transmission of motion to the tubes (2) positioned in the holder (1).
    Type: Application
    Filed: October 18, 2013
    Publication date: May 22, 2014
    Applicant: QIAGEN GMBH
    Inventors: Maximilian FOCKE, Lothar BREITKOPF, Karen KOWALEWSKI, Thorsten SINGER
  • Publication number: 20070275920
    Abstract: The invention relates to a method for chromatographic separation of a nucleic acid mixture, particularly to the separation and purification of plasmid DNA from other components of the nucleic acid mixture, particularly other nucleic acids. The inventive method is particularly characterized in that plasmid DNA can be separated without adding ribonucleases of contaminating RNA and is also characterized by the use of low-cost environmentally-friendly components. Said parameters enable the inventive method to also be used for the large-scale production of plasmid DNA. The invention also relates to the use of plasmid DNA obtained by means of the inventive method in the production of an agent containing plasmid DNA and which can be used in gene therapy and genetic vaccination.
    Type: Application
    Filed: January 25, 2005
    Publication date: November 29, 2007
    Inventors: Markus Muller, Jorg Hucklenbroich, Lothar Breitkopf