Abstract: Method for recovering a target protein from a feedstock comprising said target protein and at least one impurity compound selected from host cell proteins (HCP), DNA, RNA or other nucleic acid, the target protein being characterized by a hydrodynamic radius Rh1 and the impurity compound being characterized by a hydrodynamic radius Rh2, wherein Rh1>Rh2, comprising the following steps (i) to (iv) and optionally step (v): (i) providing a polymeric mesh comprising at least one crosslinked polymer containing positively charged amino groups, wherein the polymer has a pore size exclusion limit Rhi which can be set variably; (ii) adapting the variable pore size exclusion limit Rhi of the polymeric mesh such that Rh2<Rhi and Rh1>Rhi; (iii) contacting the polymeric mesh with the feedstock; (iv) separating the polymeric mesh containing the retained impurity compound from the feedstock containing the excluded target protein.

Abstract: Composite material comprising a support material and at least one polymeric layer, wherein the at least one polymeric layer is present in form of a polymeric mesh and is comprising at least one non-adsorbing/non-adsorptive polymer with respect to a target compound, and wherein said composite material further comprises sites which are adsorbing/adsorptive for an impurity compound; and a combination of at least one first adsorbent and at least one second adsorbent, wherein the at least one first adsorbent comprises at least one composite material comprising at least one adsorbing/adsorptive polymer, or at least one non-adsorbing/non-adsorptive polymer, or at least one adsorbing/adsorptive polymer together with at least one non-adsorbing/non-adsorptive polymer.

Abstract: Method for recovering a target protein from a feedstock comprising said target protein and at least one impurity compound selected from host cell proteins (HCP), DNA, RNA or other nucleic acid, the target protein being characterized by a hydrodynamic radius Rh1 and the impurity compound being characterized by a hydrodynamic radius Rh2, wherein Rh1>Rh2, comprising the following steps (i) to (iv) and optionally step (v): (i) providing a polymeric mesh comprising at least one crosslinked polymer containing positively charged amino groups, wherein the polymer has a pore size exclusion limit Rhi which can be set variably; (ii) adapting the variable pore size exclusion limit Rhi of the polymeric mesh such that Rh2<Rhi and Rh1>Rhi; (iii) contacting the polymeric mesh with the feedstock; (iv) separating the polymeric mesh containing the retained impurity compound from the feedstock containing the excluded target protein.

Abstract: Disclosed is a chromatographic column for use with chromatographic materials packed in a defined manner. Said chromatographic column is characterized in that the chromatographic material in a defined compressed state is enclosed in a column body (1), which is preferably cylindrical and is provided with a discharge opening (9), between two filter plates which are fixed at a specified distance from one another by contact with support surfaces situated inside the column body.

Abstract: The invention relates to a modified separating material having improved properties, to the preparation thereof, and to the use thereof for the separation of charged biopolymers from liquids.

Abstract: The invention relates to a modified separating material having improved properties, to the preparation thereof, and to the use thereof for the separation of charged biopolymers from liquids.

Abstract: Disclosed is a chromatographic column for use with chromatographic materials packed in a defined manner. Said chromatographic column is characterized in that the chromatographic material in a defined compressed state is enclosed in a column body (1), which is preferably cylindrical and is provided with a discharge opening (9), between two filter plates which are fixed at a specified distance from one another by contact with support surfaces situated inside the column body.

Abstract: The invention concerns alkali-stable hydrophilic sorbents for ion-exchange chromatography. The invention is characterized in that it consists in using as base material a crosslinked vinyl polymerconsisting of vinyl monomers containing diol groups and crosslinking monomers whereof the polymerizable groups are bound by carbonamide-containing chains. Monomers containing ionic groups are grafted on said base material using cerium (IV) salts.

Abstract: The present invention relates to affinity supports with immobilised, covalently bonded protein A, which have a particularly high functional efficiency, wherein protein A is bonded to the support via an aminocarboxylic acid as spacer, to the preparation thereof, and to the use thereof in the purification and analysis of antibodies by affinity chromatography.

Abstract: The invention concerns alkali-stable hydrophilic sorbents for ion-exchange chromatography. The invention is characterized in that it consists in using as base material a crosslinked vinyl polymerconsisting of vinyl monomers containing diol groups e and crosslinking monomers whereof the polymerizable groups are bound by carbonamide-containing chains. Monomers containing ionic groups are grafted on said base material using cerium (IV) salts.

Abstract: Continuous separation methods, in partricular using SMB methods, are disclosed, in which the analytes are separated by size exclusion chromatography (gel permeation chromatography).

Abstract: The present invention relates to a process for removing an initiator of cerium (IV) salt from a graft product. The process may include washing the graft product with a mineral acid solution comprising ascorbic acid.

Abstract: The invention relates to separating materials based on supports containing hydroxyl groups, whose surface [sic] are coated with covalently bound polymers, and to processes for their preparation. The separating materials are characterized in that the polymers consist of identical recurring units of the formula I.brket open-st.CH.sub.2 --CHX.brket close-st..sub.n (I)in whichX is CO--NH--CH.sub.2 --CH.sub.2 --SO.sub.3 H andn is 2-100.