Abstract: The invention relates to nucleotide sequences coding for the accDA gene and to a process for the preparation of L-amino acids, especially L-lysine, by fermentation using corynebacteria in which the accDA gene is amplified.

Abstract: The invention relates to a process for the preparation of L-glutamic acid by fermentation of coryneform bacteria, in which bacteria in which the nucleotide sequence which codes for D-alanine racemase (alr gene) is attenuated are employed, wherein the following steps are carried out:

Abstract: The invention relates to preferably recombinant DNA derived from Corynebacterium and replicable in coryneform microorganisms, which contains at least one nucleotide sequence that codes for the thrE gene, and a process for the production of L-threonine, which is characterised in that the following steps are carried out:

Abstract: This invention relates to a genetically modified coryneform bacterium, the cls gene of which is amplified, and to an isolated polynucleotide, which codes for cardiolipin synthase from coryneform bacteria and to a process for the fermentative production of L-amino acids with amplification of the cls gene in the bacteria and to the use of the polynucleotide as a primer or hybridization probe.

Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

Abstract: The invention relates to preferably recombinant DNA derived from Corynebacterium and replicable in coryneform microorganisms, which contains at least one nucleotide sequence that codes for the thrE gene, and a process for the production of L-threonine.

Abstract: A process for the preparation of L-amino acids, in which the following steps are carried out,

Abstract: The invention relates to an isolated polynucleotide comprising a polynucleotide sequence chosen from the group consisting of

Abstract: The invention relates to L-lysine-producing strains of corynebacteria with enhanced pyc gene (pyruvate carboxylase gene), in which strains additional genes, chosen from the group consisting of the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the lysE gene (lysine export carrier gene) and the dapB gene (dihydrodipicolinate reductase gene), but especially the dapA gene, are enhanced and, in particular, over-expressed, and to a process for the preparation of L-lysine.

Abstract: The invention provides a process for the fermentative preparation of L-amino acids using coryneform bacteria, in which the subunit carrying the biotin-carboxyl carrier protein domain and the biotin-carboxylase domain of the nucleotide sequence encoding the enzyme acetyl-CoA carboxylase (accBC gene) is amplified, in particular is overexpressed.

Abstract: The invention relates to a genetically modified coryneform bacterium, the fadD15 gene of which is amplified, and an isolated polynucleotide which codes for acyl-CoA synthase from coryneform bacteria, and also a method for the fermentative preparation of L-amino acids with amplification of the fadD15 gene in the bacteria and the use of the polynucleotide as a primer or hybridization probe.

Abstract: The invention relates to a genetically modified coryneform bacterium, the cma gene of which is amplified, and an isolated polynucleotide which codes for cyclopropane-mycolic acid synthase from coryneform bacteria, and also a method for the fermentative preparation of L-amino acids with amplification of the cma gene in the bacteria and the use of the polynucleotide as a primer or hydridization probe.

Abstract: The invention relates to nucleotide sequences coding for the accDA gene and to a process for the preparation of L-amino acids, especially L-lysine, by fermentation using corynebacteria in which the accDA gene is amplified.

Abstract: An isolated nucleic acid that encodes glycerol-3-phosphate dehydrogenase from coryneform bacteria, variants, homologs and fragments thereof. Hybridization probes and primers, vectors and host cells comprising such sequences. Coryneform bacterium with an enhanced ability to express glycerol-3-phosphate dehyrogenase. Methods of fermentative production of L-amino acids using coryneform bacteria having enhanced expression of glycerol-3-phosphate dehydrogenase.

Abstract: This invention relates to a process for the production of L-amino acids using coryneform bacteria, in which the glutamate dehydrogenase gene is amplified.

Abstract: The invention relates to L-lysine-producing strains of corynebacteria with amplified lysE gene (lysine export carrier gene), in which strains additional genes chosen from the group comprising the dapA gene (dihydrodipicolinate synthase gene), the lysC gene (aspartate kinase gene), the dapB gene (dihydrodipicolinate reductase gene) and the pyc gene, but especially the dapA gene and the lysC gene (aspartate kinase gene), are amplified and, in particular, overexpressed, and to a process for the preparation of L-lysine.

Abstract: The invention discloses three polynucleotide sequences for the fermentative production of D-pantothenic acid. These polynucleotide sequences are genes named panB, encoding a ketopantoate hydroxymethyltransferase, panC, encoding pantothenate synthase, and ilvD, encoding dihydroxy-acid dehydratase. The genes panB and panC are found on the same operon, panBC, while the gene ilvD is found in a separate operon. These genes can be used separately or together to enhance the production of D-pantothenic acid in microorganisms, especially in Corynebacterium.

Abstract: The invention relates to processes for the microbial production of L-isoleucine. To this end, in a gene in vitro of a threonine dehydratse, one or more bases in the gene region coding the enzyme's allosteric domains are exchanged in such a way that at least one amino acid in the amino acid sequence of the allosteric domains of the enzyme is replaced by another so that the enzyme is no longer inhibited by L-isoleucine feedback. Furthermore, concrete amino acid exchanges in the amino acid sequence of the enzyme are effected in a gene in vitro of a threonin dehydratase of Corynebacterium glutamicum by base exchange both outside and inside and outside the gene region coding the allosteric domains of the enzyme si that, after the transformation of such mutated threonine dehydratase genes into a threonine or L-isoleucine-producing host cell, the latter repeatedly forms L-isoleucine.

Abstract: A method for the fermentative production of L-isoleucine from D,L-.alpha.-hydroxybutyrate by means of mutants of the genus Corynbecaterium which utilize D-lactate.

Abstract: A method of producing alcohol oxidase wherein Hansenula polymorpha yeast is cultivated in a nutrient broth containing inorganic nutrient salts of which either the phosphate, nitrate or potassium is present in a cell-growth-limiting concentration at a temperature of 25.degree. to 45.degree. C. and a pH of 4.0 to 6.0 with oxygen being supplied as air for a mixture of air and oxygen. According to the invention, an organic substrate consisting of glycerin, sorbite or xylose, noncatabolite-repressive to alcohol-oxidase is added to the broth in a concentration of 0.1 to 1.0% in a nutrient solution at a rate of 0.03 to 0.15 .sup.-1.