Abstract: Provided herein are methods for culturing cells, including stimulating or expanding (proliferating), a plurality of cells in a composition of cells such as a population of lymphocytes. In some aspects, provided methods and reagents for the culturing, such as stimulation or expansion (proliferation), of cell populations involve binding of agents to a molecule on the surface of the cells, thereby providing one or more signals to the cells. In some cases, the reagents are multimerization reagents and the one or more agents are multimerized by reversibly binding to the reagent. In some aspects, the multimerized agent can provide for expansion or proliferation or other stimulation of a population of cells, and then such stimulatory agents can be removed by disruption of the reversible bond. Also provided are compositions, apparatus and methods of use thereof.

Abstract: 1. A dosing device with a pump device. 2.1. A dosing device with a pump device and with an operating handle which is provided for the pump device is known. 2.2. According to the invention, the operating handle (20, 20a, 20b) is produced as a separate component and fixed to the pump device. 2.3. Applicable for pharmaceutical agents.

Abstract: The invention relates to a dosing device with a medium reservoir. Dosing devices that comprise a medium reservoir and a pump device for dosing and dispensing a medium stored in the medium reservoir and pressure compensation means associated with the medium reservoir are known. According to the invention, the medium reservoir is provided with pressure-sensitive, flexible walls. The invention further relates to the use of said dosing device for dispensing pharmaceutical active substances.

Abstract: 1. A dosing device with a pump device.

Abstract: A piston plunger (1) is injection molded from two longitudinal sections (2, 3) in parallel axial position. The shorter component (3) is then pivoted around a hinge joint (4) into position having the same axis as the other component (2) and tightly connected to the latter using locking means (20). The shorter component (3) then forms a nozzle core (67) and the longer component (2) forms a piercing tip (68) for opening a piston membrane (75).

Abstract: In a discharge device including two mutually shiftable units (2, 3) permanent magnets (14, 24, 33, 34) are provided for controlling a piston unit (20) and valves (13, 23, 45), the magnetic force of these magnets being provided more particularly to cause a reset movement into the starting position. These magnets may be immersed in the medium or surrounded on all sides by the medium flow, thus enabling mechanical springs to be eliminated and assuring highly reliable functioning.

Abstract: A discharging apparatus (1) is arranged in substantially completely inserted or plunged manner in a storage vessel (2) with a locking flange (30), which forms an opening (40) for the at least partial reception of the operating head (20) and engages round the vessel neck (7) in the manner of a hang-in profile. The locking flange (30) carries a securing body (50) for the snap retaining of closure cap lid. Thus, there is a very compact arrangement of the discharging apparatus (1) in such a way that the latter only insignificantly increases the total length of the storage vessel (2).

Abstract: A dispenser is disclosed which is made up of two telescoping sections. One section holds at least one volume of a substance to be discharged. Telescoping motion of the sections compresses the substance to be discharged to force it through a passage in the section not holding the substance volume to be discharged therefrom. Motion between the section is limited to ensure precise metering of a charge to be dispersed.

Abstract: A dispenser is disclosed which is made up of two telescoping sections. One section holds at least one volume of a substance to be discharged. Telescoping motion of the sections compresses the substance to be discharged to force it through a passage in the section not holding the substance volume to be discharged therefrom. Motion between the section is limited to ensure precise metering of a charge to be dispersed.

Abstract: A discharge head has an operating cap and a discharge connection for fitting on the operating shaft of a discharge apparatus. The discharge head has a shaft over most of its length positioned in contact-free manner within an outer sleeve of the discharge connection, and which is provided with a connecting member for fitting on the operating shaft. The other shaft end of the discharge head shaft is only fixed by press fit in a short inner sleeve and forms a nozzle core, and at the end a free-standing end wall, at which the medium is transversely deflected from a central portion of an outlet channel and is supplied via longitudinal channels to a discharge opening.

Abstract: A discharge apparatus (1) has for the discharge actuating device (5) a self-engaging catch system (29) against further actuation at the end of each actuating cycle. For unlocking purposes an actuator (11, 23) must be manually rotated and simultaneously adjusting cams (33) of a discharge counter (20) are so transferred into an operating position, that they perform an indexing step during the next stroke.

Abstract: For restoring purposes, a discharge apparatus (1) has a gas spring (53) and for the displacement of dead volumes a filling body (70) which, like two valve bodies (23, 54) movable against one another of two valves (21, 55) with different functions, can be formed by a single component. An exposed operating ram or plunger (20), which surrounds an outlet channel (19) and is contact-free over most of its length, is constructed in one piece with a pump piston (17) and a nozzle core (48) of an outlet opening (8), while being directly connected to the spring (53). This gives high operational reliability of the discharge apparatus (1) with a simple construction.

Abstract: A dispenser for flowable media is constructed as a disposable atomizer, whose entire media reservoir is formed by the pump chamber of a thrust piston pump. The freely exposed cylindrical container constructed as a push button-like handle is therefore valve-free and can only be opened towards the outlet duct.

Abstract: Within the inner sleeve (7) of a discharge connection (5), a discharge head (1) receives approximately over its entire length the shaft (13) of an inner body (12) in such a way that the latter in the vicinity of the end of the inner sleeve (7; ) forms a connecting member (16) separate therefrom and located freely within a main body (2) serving as a plugging member for connection to a discharge apparatus.

Abstract: In a manually operable discharging apparatus (1) in the extension of the medium pump (2) provided for conveying the medium and in directly equiaxial manner at the outer end thereof is provided a compressed air pump (50) located in a cap-like, common handle (22), with which by means of a compressed air duct (90) separate from the medium supply for furthering atomization compressed air can be delivered to the discharge nozzle (25) or in other areas of the discharging apparatus (1), e.g. to the medium discharge duct (24), so that it is also possible to clean said duct by blowing free. The compressed air pump (50) can be constructed in such a way that it starts with the compressed air delivery prior to the medium delivery of the medium pump (2) and ends after this. In addition, appropriately there is an at least two-stage atomizing device (100) for the additional atomization of a preatomized medium flow with a nozzle air flow in the vicinity of discharge nozzle (25).

Abstract: In a dispenser a manually operable thrust piston pump (2) has a presuction piston (11) located with a limited spacing within a pump piston (10) and which in the manner of a telescopic tube engages over a cylindrical, freely projecting chamber body (15) and consequently bounds a presuction chamber (17) constantly connected by a line to a container and whose length and volume are significantly greater than that of a pump chamber (20) surrounding presuction chamber (17). The two working pistons slideably guided on one another form valve closures of an outlet valve (23) controlled by pressure difference and which at the end of the pump travel and in addition to said control, can be mechanically opened by a dog (40) positionally variable by means of a compressible neck (29) for venting the pump chamber (14).

Abstract: A vacuum-tight and pressure-tight closed storage space in a container is constructionally combined with a thrust piston pump projecting thereinto and a drag piston slidably guided therein and in the initial position of the thrust piston pump is hermetically sealed by an exhaust valve mechanically closed in forcibly controlled manner. The drag piston is adapted to the thrust piston pump in such a way that the storage space can be emptied substantially free with the thrust piston pump.

Abstract: A manually operable fluid dispenser is described in which the pump cylinder (12) is a separate part plugged onto a dispenser body (2) and secured against retraction by stop and counter stop members (18, 10). The pump cylinder (12) is engaging a counter member (9) of the dispenser body (2) and this counter member (9) is surrounded at a distance by an additional casing jacket (5) entirely receiving the pump cylinder (12) in the initial position of the pump piston (20). Although a resiliently opening and closing discharge valve is conceivable a discharge closure device can be provided which, upon initially operating the discharge pump, irreversibly opens. An expel member (34) immersed into the hollow pump piston (20) is provided in the pump chamber (14) in such a way that it immerses at the beginning of the pump stroke.

Abstract: A dispenser for flowable media, particularly pasty pharmaceutical or cosmetic products, has a drag piston guided in a reservoir and is filled through a connecting piece through the drag piston. A riser is connected to a discharge pump and a reservoir closure. Thus, the interior of the reservoir to be filled can be well sealed from the outside during filling and ensures a clean, hygienic filling and simple installation of the dispenser. The drag piston is advanced to close the ventilation openings with a slide type control and the riser and discharge pump are filled with the medium following the filling process.

Abstract: A dispenser or delivery apparatus for flowable media, particularly very viscous or gel-like media, has a dosing chamber body (38) located within the stored medium and the body (38) moves backwards and forwards over part of the pump stroke of the pump piston (10) of a thrust piston pump (8). Body (38) defines the piston bath (53) for pump piston (10) surrounding pump chamber (59) and the valve body (48) of an intake valve (47) for pump chamber (59). Accompanied by reliable filling of pump chamber (59) and small piston paths, this leads to a very accurate dosing of the medium quantity discharged per piston stroke.