Patents by Inventor Louis Graziano
Louis Graziano has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
-
Patent number: 9562260Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature.Type: GrantFiled: September 24, 2010Date of Patent: February 7, 2017Assignee: BIOMERIEUX, INC.Inventors: Bryan W. Kluttz, Geoff A. McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catazariti, Louis Graziano, Bruno Colin, Cecile Paris, Jacque Dachaud
-
Publication number: 20110020921Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature.Type: ApplicationFiled: September 24, 2010Publication date: January 27, 2011Applicant: bioMerieux, Inc.Inventors: Bryan W. Kluttz, Geoff A. McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catanzariti, Louis Graziano, Bruno Colin, Cecile Paris, Jacques Dachaud
-
Patent number: 7807449Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature.Type: GrantFiled: May 22, 2006Date of Patent: October 5, 2010Assignee: bioMerieux, Inc.Inventors: Bryan W. Kluttz, Geoff A. McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catazariti, Louis Graziano, Bruno Colin, Cecile Paris, Jacque Dachaud
-
Patent number: 7214529Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature.Type: GrantFiled: July 12, 2005Date of Patent: May 8, 2007Assignee: bioMérieux, Inc.Inventors: Bryan W. Kluttz, Geoff A. McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catanzariti, Louis Graziano, Bruno Colin, Cecile Jaravel, Jacques Dachaud
-
Publication number: 20060263871Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature.Type: ApplicationFiled: May 22, 2006Publication date: November 23, 2006Inventors: Bryan Kluttz, Geoff McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catanzariti, Louis Graziano, Bruno Colin, Cecile Paris, Jacques Dachuad
-
Publication number: 20050244887Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature.Type: ApplicationFiled: July 12, 2005Publication date: November 3, 2005Applicant: bioMerieux, Inc.Inventors: Bryan Kluttz, Geoff McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catanzariti, Louis Graziano, Bruno Colin, Cecile Jaravel, Jacques Dachaud
-
Patent number: 6949376Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature.Type: GrantFiled: May 7, 2002Date of Patent: September 27, 2005Assignee: bioMerieux, Inc.Inventors: Bryan W. Kluttz, Geoff A. McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catanzariti, Louis Graziano, Bruno Colin, Cecile Jaravel, Jacques Dachaud
-
Publication number: 20050197418Abstract: A polymer composition which is crosslinked and insoluble in radiation curable monomers, prepolymers, oligomers, and mixtures thereof, is suitable for formulation into radiation curable compositions, for example, as binders for formulation in radiation curable ink jet ink compositions. Such crosslinked, insoluble polymer compositions impart improved application and curing characteristics to ink jet ink compositions without loss of acceptable jettability characteristics. The polymer composition may be blended with radiation curable monomers, prepolymers, oligomers, or mixtures thereof, to form a radiation curable binder blend for formulation into a radiation curable ink jet ink composition. A radiation curable ink jet ink composition including at least one crosslinked, insoluble polymer binder composition, a liquid medium and a colorant is also provided.Type: ApplicationFiled: February 4, 2005Publication date: September 8, 2005Inventors: Louis Graziano, Richard Wu
-
Publication number: 20050197419Abstract: A polymer composition which is crosslinked and insoluble in radiation curable monomers, prepolymers, oligomers, and mixtures thereof, is suitable for formulation into radiation curable compositions, for example, as binders for formulation in radiation curable ink jet ink compositions. Such crosslinked, insoluble polymer compositions impart improved application and curing characteristics to ink jet ink compositions without loss of acceptable jettability characteristics. The polymer composition may be blended with radiation curable monomers, prepolymers, oligomers, or mixtures thereof, to form a radiation curable binder blend for formulation into a radiation curable ink jet ink composition. A radiation curable ink jet ink composition including at least one crosslinked, insoluble polymer binder composition, a liquid medium and a colorant is also provided.Type: ApplicationFiled: February 4, 2005Publication date: September 8, 2005Inventors: Louis Graziano, Richard Wu, George Lein
-
Publication number: 20020127708Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature.Type: ApplicationFiled: May 7, 2002Publication date: September 12, 2002Inventors: Bryan W. Kluttz, Geoff A. McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catanzariti, Louis Graziano, Bruno Colin, Cecile Paris, Jacques Dachaud
-
Patent number: 6429007Abstract: An instrument for conducting nucleic acid amplification reactions in a disposable test device. The test device includes a first reaction chamber containing a first nucleic acid amplification reagent (e.g., primers and nucleotides) and a second reaction chamber either containing, or in fluid communication, with a second nucleic acid amplification reagent (e.g., an amplification enzyme such as RT). The instrument includes a support structure receiving the test device. A temperature control system maintains the first reaction chamber at a first elevated temperature but simultaneously maintains the second nucleic acid amplification reagent at a second temperature lower than the first temperature so as to preserve the second nucleic acid amplification reagent. An actuator operates on a fluid conduit in the test device to place the first and second reaction chambers in fluid communication with each other after a reaction has occurred in the first reaction chamber at the first temperature.Type: GrantFiled: October 18, 1999Date of Patent: August 6, 2002Assignee: bioMérieux, Inc.Inventors: Bryan W. Kluttz, Geoff A. McKinley, Fabio Gennari, Michel Guy, Christopher Cotter, Luigi Catanzariti, Louis Graziano, Bruno Colin, Cecile Jaravel, Jacques Dachaud
-
Patent number: 6410275Abstract: A unit dose test device for a nucleic acid amplification reaction has the form of a elongate disposable test strip. The test strip includes a dual-chamber reaction vessel pre-loaded with nucleic acid amplification reaction reagents, and a plurality of wells for processing a reaction occurring in the reaction vessel.Type: GrantFiled: October 18, 1999Date of Patent: June 25, 2002Assignee: bioMerieux, Inc.Inventors: Bryan Kluttz, Lawrence Burg, Garry Tegeler, Louis Graziano, Christopher Cotter, Michel Guy, James Greer, Geoff McKinley, Luigi Catanzariti, Robert Glassfold, James Clement Bishop, Bruno Colin, Cécile Paris, Thomas Wang, Michael Morin
-
Patent number: 5786182Abstract: A reaction vessel for a nucleic acid amplification reaction has a first chamber containing an amplification reagent mix, a second chamber containing an amplification enzyme, and a fluid channel or chamber connecting the first and second chambers together. A fluid sample is introduced into the first chamber. After a denaturation and primer annealing process has occurred in the first chamber, the fluid channel is opened to allow the solution of the reagent and fluid sample to flow into the second chamber. The second chamber is maintained at an optimal temperature for the amplification reaction.A station is described for processing test strips incorporating the reaction vessels. The station includes temperature and vacuum control subsystems to maintain proper temperatures in the reaction vessel and effectuate the transfer of the fluid from one chamber to the other in an automated fashion without human intervention.Type: GrantFiled: May 2, 1997Date of Patent: July 28, 1998Assignee: bioMerieux Vitek, Inc.Inventors: Luigi Catanzariti, Bryan W. Kluttz, Geoff A. McKinley, Arthur L. Garland, Louis Graziano, James G. Moe, Marcela Vera-Garcia, James Clement Bishop, David Chastain, Fabio Gennari