Patents by Inventor Louise Fraser
Louise Fraser has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240117409Abstract: Provided herein is a method of using transposition to improve methods of sequencing RNA molecules. Provided herein is a method of tagging nucleic acid duplexes, such as DNA:RNA duplexes or DNA:DNA duplexes. The method includes the steps of providing a transposase and a transposon composition, providing one or more nucleic acid duplexes immobilized on a support, and contacting the transposase and transposon composition with the one or more nucleic acid duplexes under conditions wherein the one or more nucleic acid duplexes and transposon composition undergo a transposition reaction to produce one or more tagged nucleic acid duplexes, wherein the transposon composition comprises a double stranded nucleic acid molecule comprising a transferred strand and a non-transferred strand.Type: ApplicationFiled: October 23, 2023Publication date: April 11, 2024Applicant: Illumina Cambridge LimitedInventors: Niall Anthony Gormley, Louise Fraser, Paula Kokko-Gonzales
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Patent number: 11834699Abstract: Provided herein is a method of using transposition to improve methods of sequencing RNA molecules. Provided herein is a method of tagging nucleic acid duplexes, such as DNA:RNA duplexes or DNA:DNA duplexes. The method includes the steps of providing a transposase and a transposon composition, providing one or more nucleic acid duplexes immobilized on a support, and contacting the transposase and transposon composition with the one or more nucleic acid duplexes under conditions wherein the one or more nucleic acid duplexes and transposon composition undergo a transposition reaction to produce one or more tagged nucleic acid duplexes, wherein the transposon composition comprises a double stranded nucleic acid molecule comprising a transferred strand and a non-transferred strand.Type: GrantFiled: April 1, 2019Date of Patent: December 5, 2023Assignee: Illumina Cambridge LimitedInventors: Niall Anthony Gormley, Louise Fraser, Paula Kokko-Gonzales
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Patent number: 11634768Abstract: The invention relates to methods for indexing samples during the sequencing of polynucleotide templates, resulting in the attachment of tags specific to the source of each nucleic acid sample such that after a sequencing run, both the source and sequence of each polynucleotide can be determined. Thus, the present invention pertains to analysis of complex genomes (e.g., human genomes), as well as multiplexing less complex genomes, such as those of bacteria, viruses, mitochondria, and the like.Type: GrantFiled: February 26, 2021Date of Patent: April 25, 2023Assignee: Illumina Cambridge LimitedInventors: Helen Bignell, Louise Fraser, Niall Anthony Gormley
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Publication number: 20220002783Abstract: Presented are methods and compositions for preparing samples for amplification and sequencing. Particular embodiments relate to methods of preparing nucleic acid-containing cellular samples for library amplification, wherein the methods include lysing cells of the sample to form a lysate, amplifying the nucleic acids from the lysed samples, exposing the amplified nucleic acids to a solid surface, and clonallyr amplifying the amplified nucleic acids to generate clusters.Type: ApplicationFiled: September 15, 2021Publication date: January 6, 2022Inventors: Louise Fraser, Paula Kokko-Gonzales, Andrew Slatter
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Patent number: 11142786Abstract: Presented are methods and compositions for preparing samples for amplification and sequencing. Particular embodiments relate to methods of preparing nucleic acid-continuing cellular samples for library amplification, wherein the methods include lysing cells of the sample to form a lysate, amplifying the nucleic acids from the lysed samples, exposing the amplified nucleic acids to a solid surface, and clonally amplifying the amplified nucleic acids to generate clusters.Type: GrantFiled: August 27, 2020Date of Patent: October 12, 2021Inventors: Louise Fraser, Paula Kokko-Gonzales, Andrew Slatter
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Publication number: 20210292810Abstract: The invention relates to methods for indexing samples during the sequencing of polynucleotide templates, resulting in the attachment of tags specific to the source of each nucleic acid sample such that after a sequencing run, both the source and sequence of each polynucleotide can be determined. Thus, the present invention pertains to analysis of complex genomes (e.g., human genomes), as well as multiplexing less complex genomes, such as those of bacteria, viruses, mitochondria, and the like.Type: ApplicationFiled: February 26, 2021Publication date: September 23, 2021Inventors: Helen Bignell, Louise Fraser, Niall Anthony Gormley
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Patent number: 10988806Abstract: The invention relates to methods for indexing samples during the sequencing of polynucleotide templates, resulting in the attachment of tags specific to the source of each nucleic acid sample such that after a sequencing run, both the source and sequence of each polynucleotide can be determined. Thus, the present invention pertains to analysis of complex genomes (e.g., human genomes), as well as multiplexing less complex genomes, such as those of bacteria, viruses, mitochondria, and the like.Type: GrantFiled: June 21, 2019Date of Patent: April 27, 2021Assignee: Illumina Cambridge LimitedInventors: Helen Bignell, Louise Fraser, Niall Anthony Gormley
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Publication number: 20200392563Abstract: Presented are methods and compositions for preparing samples for amplification and sequencing. Particular embodiments relate to methods of preparing nucleic acid-continuing cellular samples for library amplification, wherein the methods include lysing cells of the sample to form a lysate, amplifying the nucleic acids from the lysed samples, exposing the amplified nucleic acids to a solid surface, and clonally amplifying the amplified nucleic acids to generate clusters.Type: ApplicationFiled: August 27, 2020Publication date: December 17, 2020Inventors: Louise Fraser, Paula Kokko-Gonzales, Andrew Slatter
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Patent number: 10774367Abstract: Presented are methods and compositions for preparing samples for amplification and sequencing. Particular embodiments relate to methods of preparing nucleic acid-containing cellular samples for library amplification, wherein the methods include providing nucleic acid containing-cellular samples from blood or FFPE samples, lysing cells of the sample to liberate nucleic acids, and performing tagmentation without purifying the liberated nucleic acids.Type: GrantFiled: October 17, 2018Date of Patent: September 15, 2020Assignee: ILLUMINA CAMBRIDGE LIMITEDInventors: Louise Fraser, Paula Kokko-Gonzales, Andrew Slatter
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Publication number: 20200080145Abstract: The invention relates to methods for indexing samples during the sequencing of polynucleotide templates, resulting in the attachment of tags specific to the source of each nucleic acid sample such that after a sequencing run, both the source and sequence of each polynucleotide can be determined. Thus, the present invention pertains to analysis of complex genomes (e.g., human genomes), as well as multiplexing less complex genomes, such as those of bacteria, viruses, mitochondria, and the like.Type: ApplicationFiled: June 21, 2019Publication date: March 12, 2020Applicant: Illumina Cambridge LimitedInventors: Helen Bignell, Louise Fraser, Niall Anthony Gormley
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Patent number: 10457985Abstract: The invention relates to methods for indexing samples during the sequencing of polynucleotide templates, resulting in the attachment of tags specific to the source of each nucleic acid sample such that after a sequencing run, both the source and sequence of each polynucleotide can be determined. Thus, the present invention pertains to analysis of complex genomes (e.g., human genomes), as well as multiplexing less complex genomes, such as those of bacteria, viruses, mitochondria, and the like.Type: GrantFiled: November 30, 2016Date of Patent: October 29, 2019Assignee: Illumina Cambridge LimitedInventors: Helen Bignell, Louise Fraser, Niall Anthony Gormley
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Publication number: 20190324042Abstract: Provided herein is a method of using transposition to improve methods of sequencing RNA molecules. Provided herein is a method of tagging nucleic acid duplexes, such as DNA:RNA duplexes or DNA:DNA duplexes. The method includes the steps of providing a transposase and a transposon composition, providing one or more nucleic acid duplexes immobilized on a support, and contacting the transposase and transposon composition with the one or more nucleic acid duplexes under conditions wherein the one or more nucleic acid duplexes and transposon composition undergo a transposition reaction to produce one or more tagged nucleic acid duplexes, wherein the transposon composition comprises a double stranded nucleic acid molecule comprising a transferred strand and a non-transferred strand.Type: ApplicationFiled: April 1, 2019Publication date: October 24, 2019Inventors: Niall Anthony Gormley, Louise Fraser, Paula Kokko-Gonzales
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Patent number: 10267804Abstract: Provided herein is a method of using transposition to improve methods of sequencing RNA molecules. Provided herein is a method of tagging nucleic acid duplexes, such as DNA:RNA duplexes or DNA:DNA duplexes. The method includes the steps of providing a transposase and a transposon composition, providing one or more nucleic acid duplexes immobilized on a support, and contacting the transposase and transposon composition with the one or more nucleic acid duplexes under conditions wherein the one or more nucleic acid duplexes and transposon composition undergo a transposition reaction to produce one or more tagged nucleic acid duplexes, wherein the transposon composition comprises a double stranded nucleic acid molecule comprising a transferred strand and a non-transferred strand.Type: GrantFiled: February 1, 2017Date of Patent: April 23, 2019Assignee: ILLUMINA CAMBRIDGE LIMITEDInventors: Niall Anthony Gormley, Louise Fraser, Paula Kokko-Gonzales
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Publication number: 20190078139Abstract: Presented are methods and compositions for preparing samples for amplification and sequencing. Particular embodiments relate to methods of obtaining nucleic acids material directly from tissues such as whole blood or FFPE samples.Type: ApplicationFiled: October 17, 2018Publication date: March 14, 2019Inventors: Louise Fraser, Paula Kokko-Gonzales, Andrew Slatter
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Publication number: 20180201974Abstract: Presented herein are methods and compositions for targeted amplification of DNA and sample identification. The methods are particularly useful in validation and quality control of samples and to confirm that WGS sequence data is properly paired with a patient sample prior to delivering sequence data to a physician or to a patient.Type: ApplicationFiled: July 5, 2016Publication date: July 19, 2018Inventor: Louise Fraser
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Publication number: 20170212123Abstract: Provided herein is a method of using transposition to improve methods of sequencing RNA molecules. Provided herein is a method of tagging nucleic acid duplexes, such as DNA:RNA duplexes or DNA:DNA duplexes. The method includes the steps of providing a transposase and a transposon composition, providing one or more nucleic acid duplexes immobilized on a support, and contacting the transposase and transposon composition with the one or more nucleic acid duplexes under conditions wherein the one or more nucleic acid duplexes and transposon composition undergo a transposition reaction to produce one or more tagged nucleic acid duplexes, wherein the transposon composition comprises a double stranded nucleic acid molecule comprising a transferred strand and a non-transferred strand.Type: ApplicationFiled: February 1, 2017Publication date: July 27, 2017Inventors: Niall Anthony Gormley, Louise Fraser, Paula Kokko-Gonzales
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Publication number: 20170145499Abstract: The invention relates to methods for indexing samples during the sequencing of polynucleotide templates, resulting in the attachment of tags specific to the source of each nucleic acid sample such that after a sequencing run, both the source and sequence of each polynucleotide can be determined. Thus, the present invention pertains to analysis of complex genomes (e.g., human genomes), as well as multiplexing less complex genomes, such as those of bacteria, viruses, mitochondria, and the like.Type: ApplicationFiled: November 30, 2016Publication date: May 25, 2017Applicant: Illumina Cambridge LimitedInventors: Helen Bignell, Louise Fraser, Niall Anthony Gormley
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Patent number: 9574226Abstract: Provided herein is a method of using transposition to improve methods of sequencing RNA molecules. Provided herein is a method of tagging nucleic acid duplexes, such as DNA:RNA duplexes or DNA:DNA duplexes. The method includes the steps of providing a transposase and a transposon composition, providing one or more nucleic acid duplexes immobilized on a support, and contacting the transposase and transposon composition with the one or more nucleic acid duplexes under conditions wherein the one or more nucleic acid duplexes and transposon composition undergo a transposition reaction to produce one or more tagged nucleic acid duplexes, wherein the transposon composition comprises a double stranded nucleic acid molecule comprising a transferred strand and a non-transferred strand.Type: GrantFiled: March 6, 2013Date of Patent: February 21, 2017Assignee: Illumina, Inc.Inventors: Niall Anthony Gormley, Louise Fraser, Paula Kokko-Gonzales
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Patent number: 9512478Abstract: The invention relates to methods for indexing samples during the sequencing of polynucleotide templates, resulting in the attachment of tags specific to the source of each nucleic acid sample such that after a sequencing run, both the source and sequence of each polynucleotide can be determined. Thus, the present invention pertains to analysis of complex genomes (e.g., human genomes), as well as multiplexing less complex genomes, such as those of bacteria, viruses, mitochondria, and the like.Type: GrantFiled: June 17, 2014Date of Patent: December 6, 2016Assignee: ILLUMINA CAMBRIDGE LIMITEDInventors: Helen Bignell, Louise Fraser, Niall Anthony Gormley
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Publication number: 20150353989Abstract: Presented are methods and compositions for preparing samples for amplification and sequencing. Particular embodiments relate to methods of obtaining nucleic acids material directly from tissues such as whole blood or FFPE samples.Type: ApplicationFiled: June 8, 2015Publication date: December 10, 2015Applicant: Illumina Cambridge LimitedInventors: Louise Fraser, Paula Kokko-Gonzales, Andrew Slatter