Abstract: A method of converting olefins to chiral epoxides comprises combining an asymmetric aliphatic or aryl alkene substrate with a buffered chloroperoxidase solution to form a stabilized reaction mixture, and gradually adding hydrogen peroxide as a substrate oxidant, such that the chloroperoxidase catalyzes the conversion of the substrate to the corresponding epoxide in enantiomeric excess. The products of the invention are alkyl and aryl non-primary epoxides. The resulting preparations are enantiomerically pure, and may greatly enhance large-scale synthesis of stereoisomer products such as pharmaceuticals and pesticides.

Abstract: A rapid, automated method for the determination of organic halogens is provided by an analytical system comprising a burner and combustion chamber for burning the organic halogen-containing sample in an oxygen-hydrogen flame; an absorption zone wherein the combustion gases are absorbed in a moving reagent film; and a colorimeter and recorder. With proper choice of reagent film, either total halogen (Cl, Br, I) content or specifically bromine content can be assayed automatically. The system is particularly useful for analysis of biological samples.