Patents by Inventor Lu-Kwang Ju

Lu-Kwang Ju has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Patent number: 11421258
    Abstract: In various aspects, the present invention is directed to a scalable method of producing rhamnolipids by bacterial fermentation with higher product concentrations, yields and productivities and preventing excessive foaming during the cell growth phase when the cell respiration rate is higher. It has been found that by slowing the growth rate of the bacteria by altering the ratio of the nitrogen source to the non-nitrogen source in the initial fermentation medium and supplementing the nitrogen source, excessive foaming in the growth phase can be prevented. Further, by using the non-nitrogen source as the limiting nutrient that initiates the stationary phase and then supplementing fermentation broth with the nitrogen and carbon sources, the length of the standing phase, and with it the time during which rhamnolipid production occurs can be greatly extended.
    Type: Grant
    Filed: August 9, 2019
    Date of Patent: August 23, 2022
    Assignee: THE UNIVERSITY OF AKRON
    Inventors: Lu-Kwang Ju, Krutika Invally
  • Patent number: 11208340
    Abstract: A multi-step method for producing an algae product comprising, a microorganism consumption step, another step, and an algae product collection step. The microorganism consumption step comprises, combining a liquid growth medium comprising microorganisms with a phagotrophic algae capable of producing a desired algae product, consuming said microorganisms by said phagotrophic algae, and growing said phagotrophic algae. Another step comprises either a microorganism growth step or a photosynthetic algal growth step. A microorganism growth step comprises providing a liquid growth medium comprising nutrients and microorganisms capable of said consuming said nutrients, consuming said nutrients by said microorganisms, and growing said microorganisms. A photosynthetic algal growth step comprises providing a substantially organic nutrient depleted liquid medium, providing a microorganism population comprising said phagotrophic algae, photosynthetic growth of said phagotrophic algae.
    Type: Grant
    Filed: December 30, 2008
    Date of Patent: December 28, 2021
    Assignee: University of Akron
    Inventor: Lu-Kwang Ju
  • Patent number: 11174501
    Abstract: A method of enzyme-based processing of plant-based materials includes steps of providing a bulk amount of a plant-based material having a substantial amount of the cell walls in a physically-intact condition; providing an enzyme broth having an enzyme capable of breaking down the cell walls in the physically-intact condition; combining the bulk amount of the plant-based material with the enzyme broth; allowing the enzyme capable of breaking down the cell walls in the physically-intact condition to break down at least a portion of the cell walls in the physically-intact condition to thereby produce intact oil bodies, hydrolyzed carbohydrates, and intact protein bodies; collecting a first product including the intact oil bodies; collecting a second product including the hydrolyzed carbohydrates; and collecting a third product including the intact protein bodies.
    Type: Grant
    Filed: December 16, 2019
    Date of Patent: November 16, 2021
    Assignee: The University of Akron
    Inventor: Lu-Kwang Ju
  • Patent number: 11066316
    Abstract: A method for treating an oil-containing medium including oil or grease or both oil and grease includes the steps of combining algae with an oil-containing medium and allowing the algae to engulf or uptake at least a portion of the oil or grease in the oil-containing medium. A mixture for treating oil or grease or both oil and grease includes an oil-containing medium including oil or grease or both oil and grease and phagotrophic algae that engulf or uptake at least a portion of the oil or grease in the oil-containing medium.
    Type: Grant
    Filed: August 1, 2014
    Date of Patent: July 20, 2021
    Assignee: The University of Akron
    Inventors: Lu-Kwang Ju, Majid Hosseini
  • Patent number: 10954276
    Abstract: The present invention is directed to enzyme based methods for removing water insoluble non-starch polysaccharides (NSPs) and/or water soluble or insoluble oligosaccharides from soy products without significantly damaging the proteins contained therein This removal is facilitated by the enzymatic hydrolysis of poly- and oligomeric carbohydrates into monosaccharides and other water soluble sugars. The present invention provides for the production of three streams of useful materials. The first is an enriched protein material comparable to the known SPCs but without significant quantities of undigestible oligosaccharides and polysaccharides. The second is an SPI made from the soluble protein in the hydrolysate which is valuable for high-quality feed, food and industrial uses. The third is the soluble saccharides and hydrolyzed carbohydrates (releasing sugars) that can be converted by fermentation to various valuable bioproducts.
    Type: Grant
    Filed: October 18, 2017
    Date of Patent: March 23, 2021
    Assignee: THE UNIVERSITY OF AKRON
    Inventors: Lu-Kwang Ju, Abdullah Loman, Anthony Coffman, Qian Li, Srujana Koganti
  • Patent number: 10696993
    Abstract: Improved enzyme based methods of separating protein from protein-rich material are provided. A method can include utilizing a modeling equation to more effectively hydrolyze the various types of carbohydrates present in a protein-rich material. A method can include a fed-batch method of incrementally adding a protein-rich material, an enzyme broth, or both a protein-rich material and an enzyme broth. A method can also include partially or completely recycling the hydrolysate.
    Type: Grant
    Filed: April 20, 2016
    Date of Patent: June 30, 2020
    Assignee: The University of Akron
    Inventors: Lu-Kwang Ju, S M Mahfuzul Islam, Qian Li, Abdullah Al Loman
  • Publication number: 20200190552
    Abstract: A method of enzyme-based processing of plant-based materials includes steps of providing a bulk amount of a plant-based material having a substantial amount of the cell walls in a physically-intact condition; providing an enzyme broth having an enzyme capable of breaking down the cell walls in the physically-intact condition; combining the bulk amount of the plant-based material with the enzyme broth; allowing the enzyme capable of breaking down the cell walls in the physically-intact condition to break down at least a portion of the cell walls in the physically-intact condition to thereby produce intact oil bodies, hydrolyzed carbohydrates, and intact protein bodies; collecting a first product including the intact oil bodies; collecting a second product including the hydrolyzed carbohydrates; and collecting a third product including the intact protein bodies.
    Type: Application
    Filed: December 16, 2019
    Publication date: June 18, 2020
    Inventor: Lu-Kwang Ju
  • Patent number: 10584361
    Abstract: A method for increasing the lipid content of algae includes combining algae and a lipid-precipitating addition in a growth-inhibitive medium, the growth-inhibitive medium being deficient in at least one nutrient that is necessary for reproductive growth of algae, thus frustrating algae reproduction, the lipid-precipitating addition selected from hydroxyl-containing compounds and amine-containing compounds. An organic acid addition may also be combined with the lipid-precipitating addition and algae in the growth-inhibitive medium, the organic acid addition including compounds containing carboxylic acid functionality. Direct production of biodiesel is also achieved by the use of particular lipid-precipitating additions and organic acid additions.
    Type: Grant
    Filed: August 5, 2013
    Date of Patent: March 10, 2020
    Assignee: THE UNIVERSITY OF AKRON
    Inventors: Lu-Kwang Ju, Cong Li
  • Publication number: 20200048673
    Abstract: In various aspects, the present invention is directed to a scalable method of producing rhamnolipids by bacterial fermentation with higher product concentrations, yields and productivities and preventing excessive foaming during the cell growth phase when the cell respiration rate is higher. It has been found that by slowing the growth rate of the bacteria by altering the ratio of the nitrogen source to the non-nitrogen source in the initial fermentation medium and supplementing the nitrogen source, excessive foaming in the growth phase can be prevented. Further, by using the non-nitrogen source as the limiting nutrient that initiates the stationary phase and then supplementing fermentation broth with the nitrogen and carbon sources, the length of the standing phase, and with it the time during which rhamnolipid production occurs can be greatly extended.
    Type: Application
    Filed: August 9, 2019
    Publication date: February 13, 2020
    Inventors: Lu-Kwang Ju, Krutika Invally
  • Patent number: 10428355
    Abstract: A method for producing arabitol may include providing a fermentation culture having a microorganism and a carbon source; allowing the microorganism to ferment the carbon source; monitoring a process condition of said step of allowing the microorganism to ferment the carbon source; and collecting a product from the fermentation culture after said step of monitoring a process condition indicates that a predetermined change in the process condition has occurred. Other methods may include steps of providing soybean-based lignocellulosic hydrolysate as a carbon source for a fermentation culture, and modifying the pH of one or more of the growth phase and the stationary phase of a fermentation process.
    Type: Grant
    Filed: July 13, 2017
    Date of Patent: October 1, 2019
    Assignee: THE UNIVERSITY OF AKRON
    Inventors: Lu-Kwang Ju, Abdullah Al Loman
  • Patent number: 10344304
    Abstract: A method of preparing a product derived from a rhamnolipid includes the steps of: providing a rhamnolipid, combining the rhamnolipid with a reagent, allowing the rhamnolipid and reagent to react to form a product derived from the rhamnolipid, and collecting the product derived from the rhamnolipid. An exemplary product is dimeric ?-hydroxy fatty acid.
    Type: Grant
    Filed: March 19, 2015
    Date of Patent: July 9, 2019
    Assignee: THE UNIVERSITY OF AKRON
    Inventors: Lu-Kwang Ju, Shida Miao
  • Patent number: 10000402
    Abstract: A method for treating solid organic materials includes providing phagotrophic algae, providing solid organic material, combining the algae and the solid organic material, allowing the algae to grow by engulfing or uptaking the solid organic material, forming an algal product, and collecting the algal product. The method can also include a pretreatment step. The solid organic material can be waste activated sludge. A system for treating and disposing solid organic material is also provided.
    Type: Grant
    Filed: May 30, 2014
    Date of Patent: June 19, 2018
    Assignee: The University of Akron
    Inventors: Lu-Kwang Ju, Cong Li, Suo Xiao
  • Patent number: 9992998
    Abstract: A biopesticide composition includes a solvent and a plurality of rhamnolipid complexes, each rhamnolipid complex including a rhamnolipid complexed with a cation. The biopesticide composition may include a slower-releasing rhamnolipid-based material and a faster-releasing rhamnolipid-based material. The biopesticide composition may be in the form of a coating or a pellet.
    Type: Grant
    Filed: March 20, 2017
    Date of Patent: June 12, 2018
    Assignee: The University of Akron
    Inventors: Lu-Kwang Ju, Ashwin Sancheti
  • Publication number: 20180118794
    Abstract: The present invention is directed to enzyme based methods for removing water insoluble non-starch polysaccharides (NSPs) and/or water soluble or insoluble oligosaccharides from soy products without significantly damaging the proteins contained therein This removal is facilitated by the enzymatic hydrolysis of poly- and oligomeric carbohydrates into monosaccharides and other water soluble sugars. The present invention provides for the production of three streams of useful materials. The first is an enriched protein material comparable to the known SPCs but without significant quantities of undigestible oligosaccharides and polysaccharides. The second is an SPI made from the soluble protein in the hydrolysate which is valuable for high-quality feed, food and industrial uses. The third is the soluble saccharides and hydrolyzed carbohydrates (releasing sugars) that can be converted by fermentation to various valuable bioproducts.
    Type: Application
    Filed: October 18, 2017
    Publication date: May 3, 2018
    Applicant: THE UNIVERSITY OF AKRON
    Inventors: Lu-Kwang JU, Abdullah Loman, Anthony Coffman, Qian Li, Srujana Koganti
  • Publication number: 20180016603
    Abstract: A method for producing arabitol may include providing a fermentation culture having a microorganism and a carbon source; allowing the microorganism to ferment the carbon source; monitoring a process condition of said step of allowing the microorganism to ferment the carbon source; and collecting a product from the fermentation culture after said step of monitoring a process condition indicates that a predetermined change in the process condition has occurred. Other methods may include steps of providing soybean-based lignocellulosic hydrolysate as a carbon source for a fermentation culture, and modifying the pH of one or more of the growth phase and the stationary phase of a fermentation process.
    Type: Application
    Filed: July 13, 2017
    Publication date: January 18, 2018
    Inventors: Lu-Kwang Ju, Abdullah Al Loman
  • Patent number: 9809630
    Abstract: The present invention is directed to enzyme based methods for separating protein from protein-rich materials derived from plant seeds, fruit, or other biomass and products made therefrom. The protein content in the resulting products is improved by separating and removing the carbohydrates from around the proteins in, for example, soybean meal. This removal is facilitated by the enzymatic hydrolysis of poly- and oligomeric carbohydrates into monosaccharides and other water soluble sugars. The present invention provides for the production of three streams of useful materials. The first is an enriched protein material comparable to the known SPCs but without significant quantities of undigestible oligosaccharides and polysaccharides. The second is an SPI made from the soluble protein in the hydrolysate which is valuable for high-quality feed, food and industrial uses.
    Type: Grant
    Filed: May 9, 2013
    Date of Patent: November 7, 2017
    Assignee: THE UNIVERSITY OF AKRON
    Inventors: Lu-Kwang Ju, Abdullah Loman, Anthony Coffman, Qian Li, Srujana Koganti
  • Patent number: 9783442
    Abstract: Phagotrophic algae are used in connection with aerobic or anaerobic digestion of solids, especially waste activated sludge (WAS), to more efficiently digest solids and to meet, over a shorter period of time, volatile solids standards and specific oxygen uptake rate requirements as well as pathogen reduction requirements.
    Type: Grant
    Filed: September 25, 2015
    Date of Patent: October 10, 2017
    Assignees: Ovivo Inc., The University of Akron
    Inventors: Lu-Kwang Ju, Hiren K. Trivedi
  • Publication number: 20170265468
    Abstract: A biopesticide composition includes a solvent and a plurality of rhamnolipid complexes, each rhamnolipid complex including a rhamnolipid complexed with a cation. The biopesticide composition may include a slower-releasing rhamnolipid-based material and a faster-releasing rhamnolipid-based material. The biopesticide composition may be in the form of a coating or a pellet.
    Type: Application
    Filed: March 20, 2017
    Publication date: September 21, 2017
    Inventors: Lu-Kwang Ju, Ashwin Sancheti
  • Publication number: 20170175151
    Abstract: A method of preparing a product derived from a rhamnolipid includes the steps of: providing a rhamnolipid, combining the rhamnolipid with a reagent, allowing the rhamnolipid and reagent to react to form a product derived from the rhamnolipid, and collecting the product derived from the rhamnolipid. An exemplary product is dimeric ?-hydroxy fatty acid.
    Type: Application
    Filed: March 19, 2015
    Publication date: June 22, 2017
    Inventors: Lu-Kwang Ju, Shida Miao
  • Publication number: 20160304925
    Abstract: Improved enzyme based methods of separating protein from protein-rich material are provided. A method can include utilizing a modeling equation to more effectively hydrolyze the various types of carbohydrates present in a protein-rich material. A method can include a fed-batch method of incrementally adding a protein-rich material, an enzyme broth, or both a protein-rich material and an enzyme broth. A method can also include partially or completely recycling the hydrolysate.
    Type: Application
    Filed: April 20, 2016
    Publication date: October 20, 2016
    Inventor: Lu-Kwang Ju