Patents by Inventor Luc Bousse
Luc Bousse has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Patent number: 12174144Abstract: Methods, devices, and systems for improving the quality of electrospray ionization mass spectrometer (ESI-MS) data are described, as are methods, devices, and systems for achieving improved correlation between chemical separation data and mass spectrometry data.Type: GrantFiled: October 11, 2022Date of Patent: December 24, 2024Assignee: Intabio, LLCInventors: Erik Gentalen, Steve Lacy, Scott Mack, Luc Bousse, Morten Jensen
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Publication number: 20240377359Abstract: Methods, devices, and systems for improving the quality of electrospray ionization mass spectrometer (ESI-MS) data are described, as are methods, devices, and systems for achieving improved correlation between chemical separation data and mass spectrometry data.Type: ApplicationFiled: July 21, 2024Publication date: November 14, 2024Inventors: Luc Bousse, Erik Gentalen, Morten Jensen, Steve Lacy, Alexander Petrov
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Publication number: 20240165612Abstract: This disclosure describes various microfluidic devices that may be used in thermal cyclic fluid samples. Some of these devices may include a plurality of microwells that may be coupled by interconnected fluidic channels. These microwells may not be physically separated and yet may include features allowing for effective isolation of target molecules within each microwell. Other devices may include a plurality of microwells that may not be interconnected. The devices may also include mechanisms for causing a fluid to flow across the device. The devices may also include light-absorbing films for converting light energy to heat so as to allow for thermal cycling of samples within the microwells.Type: ApplicationFiled: June 23, 2023Publication date: May 23, 2024Inventors: Jun Ho Son, Luc Bousse
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Patent number: 11224875Abstract: Methods, devices, and systems for performing isoelectric focusing reactions are described. The systems or devices disclosed herein may comprise fixtures that have a membrane. In some instances, the disclosed devices may be designed to perform isoelectric focusing or other separation reactions followed by further characterization of the separated analytes using mass spectrometry. The disclosed methods, devices, and systems provide for fast, accurate separation and characterization of protein analyte mixtures or other biological molecules by isoelectric point.Type: GrantFiled: December 16, 2020Date of Patent: January 18, 2022Assignee: INTABIO, LLCInventors: Eric Gwerder, Scott Mack, Erik Gentalen, Luc Bousse, Don Wesley Arnold
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Publication number: 20210008550Abstract: This disclosure describes various microfluidic devices that may be used in thermal cyclic fluid samples. Some of these devices may include a plurality of microwells that may be coupled by interconnected fluidic channels. These microwells may not be physically separated and yet may include features allowing for effective isolation of target molecules within each microwell. Other devices may include a plurality of microwells that may not be interconnected. The devices may also include mechanisms for causing a fluid to flow across the device. The devices may also include light-absorbing films for converting light energy to heat so as to allow for thermal cycling of samples within the microwells.Type: ApplicationFiled: July 8, 2020Publication date: January 14, 2021Inventors: Jun Ho Son, Luc Bousse
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Patent number: 10870113Abstract: Methods, devices, and systems for performing isoelectric focusing reactions are described. The systems or devices disclosed herein may comprise fixtures that have a membrane. In some instances, the disclosed devices may be designed to perform isoelectric focusing or other separation reactions followed by further characterization of the separated analytes using mass spectrometry. The disclosed methods, devices, and systems provide for fast, accurate separation and characterization of protein analyte mixtures or other biological molecules by isoelectric point.Type: GrantFiled: March 3, 2020Date of Patent: December 22, 2020Assignee: INTABIO, INC.Inventors: Erik Gentalen, Scott Mack, Eric Gwerder, Luc Bousse
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Patent number: 10682647Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In an exemplary method, a sample-containing fluid may be dispensed into a well through a sample port of a channel component. The channel component may include (a) a body having a bottom surface attached to the well, and a top surface with a microchannel formed therein, and (b) an input tube projecting into the well from the bottom surface of the body. The sample-containing fluid when dispensed may contact a bottom end of the input tube and may be retained, with assistance from gravity, out of contact with the microchannel. A pressure differential may be created that drives at least a portion of the sample-containing fluid from the well via the input tube and through the microchannel.Type: GrantFiled: June 26, 2017Date of Patent: June 16, 2020Assignee: Bio-Rad Laboratories, Inc.Inventors: Anthony J. Makarewicz, Jr., Luc Bousse, Stefano Schiaffino
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Patent number: 10514360Abstract: Methods, devices, and systems for improving the quality of electrospray ionization mass spectrometer (ESI-MS) data are described, as are methods, devices, and systems for achieving improved correlation between chemical separation data and mass spectrometry data.Type: GrantFiled: August 6, 2019Date of Patent: December 24, 2019Assignee: INTABIO, INC.Inventors: Erik Gentalen, Steve Lacy, Scott Mack, Luc Bousse, Morten Jensen
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Publication number: 20190369048Abstract: Methods, devices, and systems for improving the quality of electrospray ionization mass spectrometer (ESI-MS) data are described, as are methods, devices, and systems for achieving improved correlation between chemical separation data and mass spectrometry data.Type: ApplicationFiled: August 6, 2019Publication date: December 5, 2019Inventors: Erik Gentalen, Steve Lacy, Scott Mack, Luc Bousse, Morten Jensen
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Publication number: 20190369068Abstract: Methods, devices, and systems for improving the quality of electrospray ionization mass spectrometer (ESI-MS) data are described, as are methods, devices, and systems for achieving improved correlation between chemical separation data and mass spectrometry data.Type: ApplicationFiled: May 31, 2019Publication date: December 5, 2019Inventors: Erik Gentalen, Steve Lacy, Scott Mack, Luc Bousse, Morten Jensen
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Publication number: 20170291174Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In an exemplary method, a sample-containing fluid may be dispensed into a well through a sample port of a channel component. The channel component may include (a) a body having a bottom surface attached to the well, and a top surface with a microchannel formed therein, and (b) an input tube projecting into the well from the bottom surface of the body. The sample-containing fluid when dispensed may contact a bottom end of the input tube and may be retained, with assistance from gravity, out of contact with the microchannel. A pressure differential may be created that drives at least a portion of the sample-containing fluid from the well via the input tube and through the microchannel.Type: ApplicationFiled: June 26, 2017Publication date: October 12, 2017Inventors: Anthony J. Makarewicz, JR., Luc Bousse, Stefano Schiaffino
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Patent number: 9687848Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In an exemplary method, a sample-containing fluid may be dispensed into a well through a sample port of a channel component. The channel component may include (a) a body having a bottom surface attached to the well, and a top surface with a microchannel formed therein, and (b) an input tube projecting into the well from the bottom surface of the body. The sample-containing fluid when dispensed may contact a bottom end of the input tube and may be retained, with assistance from gravity, out of contact with the microchannel. A pressure differential may be created that drives at least a portion of the sample-containing fluid from the well via the input tube and through the microchannel.Type: GrantFiled: August 8, 2016Date of Patent: June 27, 2017Assignee: Bio-Rad Laboratories, Inc.Inventors: Anthony J. Makarewicz, Jr., Luc Bousse, Stefano Schiaffino
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Publication number: 20160339436Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In an exemplary method, a sample-containing fluid may be dispensed into a well through a sample port of a channel component. The channel component may include (a) a body having a bottom surface attached to the well, and a top surface with a microchannel formed therein, and (b) an input tube projecting into the well from the bottom surface of the body. The sample-containing fluid when dispensed may contact a bottom end of the input tube and may be retained, with assistance from gravity, out of contact with the microchannel. A pressure differential may be created that drives at least a portion of the sample-containing fluid from the well via the input tube and through the microchannel.Type: ApplicationFiled: August 8, 2016Publication date: November 24, 2016Inventors: Anthony J. Makarewicz, JR., Luc Bousse, Stefano Schiaffino
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Patent number: 9409174Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In some embodiments, the system may include a well and a channel component attached to the well. The channel component may include (a) a body, (b) an input tube (a “fluid pickup”) projecting from a bottom surface of the body and having an open bottom end disposed in the input well, (c) a microchannel, and (d) a passage extending through the input tube and the body and connecting the well to the microchannel. The system may be configured to receive a sample-containing fluid in the well and retain the sample-containing fluid below a top end of the passage, until a pressure differential is created that drives at least a portion of the sample-containing fluid from the well via the passage and through the microchannel.Type: GrantFiled: June 23, 2014Date of Patent: August 9, 2016Assignee: Bio-Rad Laboratories, Inc.Inventors: Anthony J. Makarewicz, Jr., Luc Bousse, Stefano Schiaffino
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Patent number: 9399215Abstract: Sample holding system, including methods and apparatus, including a holder defining a well having a wicking promoter that encourages flow of a sample to the bottom of the well. Methods of making and using the holder are also disclosed.Type: GrantFiled: April 15, 2013Date of Patent: July 26, 2016Assignee: Bio-Rad Laboratories, Inc.Inventors: Thomas H. Cauley, III, Luc Bousse, Jonathan Petersen, Kevin D. Ness, Steven Romine
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Publication number: 20140378348Abstract: Microfluidic system, including methods and apparatus, for processing fluid, such as by droplet generation. In some embodiments, the system may include a well and a channel component attached to the well. The channel component may include (a) a body, (b) an input tube (a “fluid pickup”) projecting from a bottom surface of the body and having an open bottom end disposed in the input well, (c) a microchannel, and (d) a passage extending through the input tube and the body and connecting the well to the microchannel. The system may be configured to receive a sample-containing fluid in the well and retain the sample-containing fluid below a top end of the passage, until a pressure differential is created that drives at least a portion of the sample-containing fluid from the well via the passage and through the microchannel.Type: ApplicationFiled: June 23, 2014Publication date: December 25, 2014Inventors: Anthony J. Makarewicz, JR., Luc Bousse, Stefano Schiaffino
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Publication number: 20140024023Abstract: System, including methods and apparatus, for forming droplets of an emulsion. The system may include a channel junction at which a stream of sample fluid is divided into droplets by a dividing flow of carrier fluid. The system also may include one or more features configured to position sample fluid for reduced contact between the sample fluid and one or more surface regions of the channel junction, which may improve the consistency of droplet formation. In exemplary embodiments, sample fluid may be positioned by a step member produced by an increase in channel depth, and/or by directing flow of carrier fluid to form a barrier layer between sample fluid and a wall region, such as a ceiling region or floor region, of a channel network.Type: ApplicationFiled: July 23, 2013Publication date: January 23, 2014Applicant: Bio- Rad Laboratories, IncInventors: Thomas H. Cauley, III, Timothy Brackbill, Luc Bousse, Jon Petersen
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Publication number: 20130269452Abstract: Sample holding system, including methods and apparatus, including a holder defining a well having a wicking promoter that encourages flow of a sample to the bottom of the well. Methods of making and using the holder are also disclosed.Type: ApplicationFiled: April 15, 2013Publication date: October 17, 2013Inventors: Thomas H. Cauley III, Luc Bousse, Jon Petersen, Kevin D. Ness, Steven Romine
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Patent number: 8394324Abstract: A microfluidic device is provided with appropriate integrated structures to conduct large volume PCR and end-point or real-time capillary electrophoresis detection. The microfluidic device includes a substrate having an amplification chamber of a volume of nucleic acid, wells disposed on the substrate, flow channels connecting the wells and the chamber in the substrate to allow for solution flow through the chamber, and one or more separation channels provided in the substrate and connected to the chamber for separating and detecting a fraction of the amplified nucleic acid. The chamber, the flow channels, and the one or more separation channels are configured such that the hydrodynamic flow resistance of the chambers and the flow channels combined is at least 10?3 times smaller than the hydrodynamic flow resistance in the one or more separation channels. The microfluidic device can achieve a very high sensitivity in detection while being highly cost effective.Type: GrantFiled: June 11, 2008Date of Patent: March 12, 2013Assignee: Wako Pure Chemical Industries, Ltd.Inventors: Luc Bousse, Jian-ping Zhang
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Publication number: 20100173310Abstract: A microfluidic device is provided with appropriate integrated structures to conduct large volume PCR and end-point or real-time capillary electrophoresis detection The microfluidic device includes a substrate having an amplification chamber of a volume of nucleic acid, wells disposed on the substrate, flow channels connecting the wells and the chamber in the substrate to allow for solution flow through the chamber, and one or more separation channels provided in the substrate and connected to the chamber for separating and detecting a fraction of the amplified nucleic acid The chamber, the flow channels, and the one or more separation channels are configured such that the hydrodynamic flow resistance of the chambers and the flow channels combined is at least 10?3 times smaller than the hydrodynamic flow resistance in the one or more separation channels The microfluidic device can achieve a very high sensitivity in detection while being highly cost effectiveType: ApplicationFiled: June 11, 2008Publication date: July 8, 2010Inventors: Luc Bousse, Jian-ping Zhang