Abstract: The present invention relates to cell and tissue culture. In particular, the present invention provides a method for preparing an organotypic culture using dissociated cells or microexplants obtained from an animal organ. The method for preparing an organotypic culture comprises culturing cells from an organ on a surface characterized in that the cells are compacted. The invention further relates to a high-throughput method for the preparation of a collection of organotypic cultures. The invention further relates to a device for carrying out a method of organotypic culture according to the invention.

Abstract: The invention relates to the field of cell and tissue culture. In particular, the invention relates to devices for cell culture. A device for cell culture, according to the invention comprises (i) a medium conduit having one open end and one end closed by a porous membrane fused across it; and (ii) a frame holding the medium conduit in a substantially vertical orientation; wherein the medium conduit is adapted to permit retention by capillarity of a sufficient volume of liquid culture medium in the medium conduit to contact the surface of the porous membrane and thus supply nutrients to cells that may be grown on the porous membrane, characterised in that the surface of the membrane contralateral to the surface of said porous membrane sealed to said medium conduit comprises a hydrophobic barrier adapted to contain the culture.

Abstract: The invention relates to the field of cell and tissue culture. In particular, the invention provides methods for culturing cells to form aggregates, including stem cells and primary cells. A method for culturing cells according to the invention comprises the steps of: (i) incubating a cells in a hanging drop on the underside of a porous membrane to form aggregates of cells; (ii) inverting the membrane so that the aggregates of cells are located on the upperside of the membrane; and (iii) incubating the aggregates of cells on the upperside of the membrane.

Abstract: The present invention relates to the field of cell and tissue culture. In particular, the invention provides a novel device for organotypic culture. The device comprises a medium conduit having one open end and one end closed by a porous membrane fused across it; and a frame holding the medium conduit in a substantially vertical orientation; wherein the medium conduit is adapted to permit retention by capillarity of a sufficient volume of liquid culture medium in the medium conduit to contact the surface of the porous membrane and thus supply nutrients to cells that may be grown on the porous membrane. The device may further comprise a cell suspension conduit having one end open and one end closed by the surface of said porous membrane contralateral to the surface of said porous membrane sealed to said medium conduit; wherein said frame holds the medium conduit and the cell suspension conduit in a substantially vertical orientation.

Abstract: The present invention relates to the field of cell and tissue culture. In particular, the invention provides a novel device for organotypic culture. The device comprises a medium conduit having one open end and one end closed by a porous membrane fused across it; and a frame holding the medium conduit in a substantially vertical orientation; wherein the medium conduit is adapted to permit retention by capillarity of a sufficient volume of liquid culture medium in the medium conduit to contact the surface of the porous membrane and thus supply nutrients to cells that may be grown on the porous membrane. The device may further comprise a cell suspension conduit having one end open and one end closed by the surface of said porous membrane contralateral to the surface of said porous membrane sealed to said medium conduit; wherein said frame holds the medium conduit and the cell suspension conduit in a substantially vertical orientation.

Abstract: The present invention relates to cell and tissue culture. In particular, the present invention provides a method for preparing an organotypic culture using dissociated cells or microexplants obtained from an animal organ. The method for preparing an organotypic culture comprises culturing cells from an organ on a surface characterised in that the cells are compacted. The invention further relates to a high-throughput method for the preparation of a collection of organotypic cultures. The invention further relates to a device for carrying out a method of organotypic culture according to the invention.

Abstract: The invention concerns an apparatus (10) comprising essentially a support (11) wherein is placed a measuring card (12), a perfusion device (13), a thermostat (14), a measuring box (15) and a measurement management system (16). The measuring card (12) comprises an electrode array whereof one end emerges into the chamber and the other end is placed outside said chamber. The group of cells is placed in the chamber which is connected to the perfusion device (13). The support (11) comprises a connector (25) connecting the card (12) electrodes to the measurement management system (16) via the measuring box (15).

Abstract: The invention concerns a device comprising a support (11) in the thickness of which is produced a supply chamber (12) with an inlet duct (13) for nutrient liquid and a discharge duct (14) for said liquid. A capsule (22) capable of receiving organic cells is provided on the support (11). The supply chamber (12) and the support (11) are separated by a porous membrane (16) comprising an array of electrodes (17) arranged so as to be in contact with different zones of the group of cells, thereby enabling their electrophysiological activity to be analyzed. The device enables to increase the life span of cells and to carry out analyses simply and without affecting the cell organization.

Abstract: A device for culturing dissociated cultures or organotypic cultures on pieces of pre-cut membrane comprising discs of membrane which are circled by a plastic ring provided with a tab and the patch of membrane which is placed in a culture medium selected from the group consisting of in a culture insert, on a semi-solid medium and on the membrane of a box, consisting of a case made of transparent plastic material formed by two assembled parts which are hermetically sealed, the lower part being separated into two compartments by a permeable membrane defining a lower compartment which serves as a reservoir for the culture medium and an upper compartment intended to receive one or more circled membrane discs or eyelets, serving as the site for the cell culture and its use.

Abstract: Device for studying and recording the electrophysiological phenomena in cultures of excitable tissues, said device comprising a combination of a lower half card comprising a perfusion medium in a perfusion chamber sealed by a permeable and transparent membrane and an upper half card with a flexible printed circuit bearing a network of electrodes and fitted with a cap for insuring the closure of the device and a method of use thereof.