Abstract: A method (300) at an imaging acquisition system (100), comprising: obtaining a sequence of reflectance images (401-406); acquiring a sequence of fluorescence channel images (601-603) including light images (602), acquired while using a light source to cause a target body-part to emit fluorescent light, alternating with dark images (601, 603), acquired without using the light source; determining (304, 310) a first reflectance image (401) associated with a first fluorescence channel image (601) having a first reference time, and a second reflectance image (403) associated with a consecutive second fluorescence channel image (602) having a second reference time; determining (311) a transformation matrix based on a relative alignment of the first and second reflectance images; applying (315) the transformation matrix to one of the first and second fluorescence channel images to align these images; performing a subtraction (316) between the fluorescence channel image to which the transformation matrix has been app

Abstract: The present invention provides a method for generating somatic stem cells out of differentiated cells, somatic stem cells obtained by this method and a vector or composition for use in this method.