Abstract: The invention is directed to a method for recovering a lipid or hydrocarbon from a fermentation mixture, comprising the steps of—providing a fermentation mixture wherein the lipid or hydrocarbon is produced by microbial fermentation in a fermentation vessel, which mixture comprises an aqueous phase and a liquid product phase, wherein the liquid product phase comprises the lipid or hydrocarbon; and—feeding at least part of the aqueous phase and part of the liquid product phase to a second vessel, thereby forming a second mixture; and—promoting phase-separation of the aqueous and product phase by injecting a gas into the second mixture, thereby separating the product phase from the aqueous phase; and—collecting the product phase comprising the lipid or hydrocarbon.

Abstract: The invention is directed to a method for recovering a lipid or hydrocarbon from a fermentation mixture, comprising the steps of—providing a fermentation mixture wherein the lipid or hydrocarbon is produced by microbial fermentation in a fermentation vessel, which mixture comprises an aqueous phase and a liquid product phase, wherein the liquid product phase comprises the lipid or hydrocarbon; and—feeding at least part of the aqueous phase and part of the liquid product phase to a second vessel, thereby forming a second mixture; and—promoting phase-separation of the aqueous and product phase by injecting a gas into the second mixture, thereby separating the product phase from the aqueous phase; and—collecting the product phase comprising the lipid or hydrocarbon.

Abstract: The invention relates to a method of preparing a protein aggregate, wherein an aqueous protein solution is acidified with a pH that lies above the isoelectric point of the protein. In accordance with the invention, a first protein, which through acidification is able to form a protein aggregate, is acidified in the presence of a second protein in the aqueous solution in order to form a coaggregate comprising the first and second protein, wherein the second protein under identical temperature conditions and pH does not form a protein aggregate in the absence of the first protein. Acidification preferably occurs with the aid of CO2.

Abstract: The invention relates to a method of preparing a protein aggregate, wherein an aqueous protein solution is acidified with a pH that lies above the isoelectric point of the protein. In accordance with the invention, a first protein, which through acidification is able to form a protein aggregate, is acidified in the presence of a second protein in the aqueous solution in order to form a coaggregate comprising the first and second protein, wherein the second protein under identical temperature conditions and pH does not form a protein aggregate in the absence of the first protein. Acidification preferably occurs with the aid of CO2.

Abstract: The invention relates to a process for the preparation of an aqueous solution or suspension of a &bgr;-lactam nucleus and application thereof. The aqueous solution or suspension of the &bgr;-lactam nucleus is prepared by process wherein an enzymatic deacylation of a &bgr;-lactam compound, which compounds comprises a &bgr;-lactam nucleus with a side chain coupled to it via an amide bond and which deacylation reaction is carried out in a mixture of water and an organic solvent and which deacylation leads to a &bgr;-lactam nucleus and a carboxylic acid, is carried out at a pH value of between 2 and 6 so that the carboxylic acid is extracted in situ to the organic solvent.

Abstract: A method for isolating casein and calcium phosphate as separate products from a milk source, comprising the steps of: a) contacting the milk source with carbon dioxide under pressure in order to precipitate the casein; b) separating the casein from the milk source while maintaining the pressure to obtain a casein fraction and a whey fraction; and c) releasing the pressure from the whey fraction to allow calcium phosphate to precipitate therefrom.

Abstract: The present invention relates to a method of separating a first solute A and a second solute B using (simulated) moving bed chromatography. According to the present invention at least one of a) a feed stream; and b) a desorbent stream comprises an organic solvent. The use of different solvent liquids for the feedstream and the desorbent stream results in an increased difference in partition coefficients for solute A and solute B. According to the present invention it is possible to obtain solute A in an extract stream in a form which is more concentrated in comparison to the concentration of solute A in the feedstream.