Abstract: A hyperspectral imaging system that includes an image capture device, an illumination component, a tunable filter, and an infrared cut-off filter. The hyperspectral system can capture a spectral image of a target object such as a clinical test subject across a spectral range of at least 450 nm to 700 nm at a spectral resolution of at least 50 nm. The infrared cut-off filter is positioned between the target object and the tunable filter to reduce leak-through and improve the performance of the hyperspectral imaging system.