Abstract: The disclosed method may include configuring one or more brakes based on a braking-related attribute expected at a geographic location being traversed by a personal mobility vehicle. By configuring the application of brakes of a personal mobility vehicle based on terrain, environmental conditions, and other geographic features, the system may reduce the risk of the vehicle skidding or tipping due to over-braking. In some embodiments, a rider may use a single brake lever to indicate a desire to brake and the system may make determinations about how to apply a combination of mechanical and electrical brakes to front and back wheels based at least in part on the location of the personal mobility vehicle. By configuring brake engagement based on a combination of controls and map data, the system may improve user experience and user safety, especially for inexperienced riders.

Abstract: A flow cell includes a support and a heteropolymer attached to the support. The heteropolymer includes an acrylamide monomer including an attachment group to react with a functional group attached to a primer, and a monomer including a stimuli-responsive functional group. The monomer including the stimuli-responsive functional group may be pH-responsive, temperature-responsive, saccharide-responsive, nucleophile-responsive, and/or salt-responsive.

Abstract: A system for multiple brakes intelligently controlled by a single brake input on a personal mobility vehicle. By determining a front and rear brake differential based on the position and weight of the rider as well as the environmental and vehicle conditions, the system may reduce the risk of the vehicle skidding or tipping due to over-braking. In some embodiments, a rider may use a single brake lever to indicate a desire to brake and the system may make determinations about how to apply a combination of mechanical and electrical brakes to front and back wheels. By applying different braking systems based on a combination of controls and sensors, the system may improve user experience and user safety, especially for inexperienced riders.

Abstract: An example primer set includes first and second nuclease resistant primers. The first nuclease resistant primer includes a first sequence; a first cleavage site attached at a 3? end of the first sequence; and a first nuclease resistant modification incorporated between the first sequence and the first cleavage site. The second nuclease resistant primer includes a second sequence that is different from the first sequence; a second nuclease resistant modification incorporated at a 3? end of the second sequence; and a second cleavage site attached between the second sequence and the second nuclease resistant modification. The second cleavage site is different from the first cleavage site.

Abstract: A micromobility vehicle for use in a vehicle share system includes a frame, a footboard fixed to the frame, a seat fixed to the frame, handlebars, and a computing device. The frame includes a horizontal portion and an angled portion. The seat is separated from the footboard in a vertical direction by a fixed distance. The handlebars are separated from the footboard in the vertical direction by a fixed distance. A cargo storage location is at least partially disposed between the seat and the footboard. The computing device is fixed to the frame and capable of executing a transportation matching system application.

Abstract: Multiscale lens systems and methods for imaging well plates and including event-based detection is disclosed. In some embodiments, the multiscale lens systems and methods provide a multiple read-head optical detection system, a step-and-shoot optical detection system, a multiscale microlens optical detection system, and/or a multiscale GRIN lens optical detection system. Further, a fluidics system may include a liquid handling system and a multiscale optical detection system in relation to a well plate. Further, a method of using a multiple read-head optical detection system is provided. Further, a method of using a step-and-shoot optical detection system is provided. Further, a method of using a lens array optical detection system is provided.

Abstract: A system for multiple brakes intelligently controlled by a single brake input on a personal mobility vehicle. By determining a front and rear brake differential based on the position and weight of the rider as well as the environmental and vehicle conditions, the system may reduce the risk of the vehicle skidding or tipping due to over-braking. In some embodiments, a rider may use a single brake lever to indicate a desire to brake and the system may make determinations about how to apply a combination of mechanical and electrical brakes to front and back wheels. By applying different braking systems based on a combination of controls and sensors, the system may improve user experience and user safety, especially for inexperienced riders.

Abstract: Methods are provided for conducting an assay for a set of targets. The methods include (a) performing a methylation sensitive transformation process on a sample comprising a set of target nucleic acid sequences; (b) performing a hybridization and ligation reaction with an encoded detection probe from a set of encoded detection probes unique for each of the targets, the probes comprising a nucleotide sequence code from a set of nucleotide sequence codes to generate an amplifiable probe, wherein the probes unique for unmethylated targets are not amplifiable; and (c) performing an amplification and detection process on the sample, wherein the methylated targets are detected by decoding the amplified codes associated with the methylated targets.

Abstract: Methods for assaying polypeptide targets are provided that include: (a) binding each target in a sample potentially comprising a set of polypeptide targets to a capture agent to yield a set of capture agent-target complexes; (b) performing a recognition event on the capture agent-target complexes to yield capture agent-target complexes comprising an encoded probe, the encoded probe comprising a code from a set of codes, each code comprising at least one segment encoding one or more symbols that correspond to a sequence of one or more nucleotides; (c) performing a molecular transformation event to produce modified encoded probes in the presence of the target and unmodified encoded probes in the absence of the target, in which the modified probes can be amplified and the unmodified probes cannot be amplified in an amplification event; and (d) performing the amplification event and detecting the targets by decoding the codes that are amplified.

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

Abstract: A method of conducting an assay for a set of targets, comprising: subjecting each target of a set of targets to a recognition event, in which each target is uniquely recognized by and bound to a recognition element associated with a code from a set of codes, thereby yielding a set of coded targets comprising the target and the recognition element; subjecting each recognition element of the set of coded targets to a transformation event, in which a molecular transformation of each recognition element produces a modified recognition element, thereby yielding a set of modified recognition elements comprising the code; subjecting each code of the set of modified recognition elements to an amplifying event, in which each code is amplified, thereby yielding a set of amplified codes; subjecting each amplified code of the set of amplified codes to a detection event, thereby decoding the code.

Abstract: A micromobility electric vehicle includes a front storage volume and a rear storage volume. The front storage volume may be defined on two sides by an angled footboard portion and a column. The rear storage volume may be defined on two sides by a curved footboard portion and a seat post. Retainers including an elastic member may be employed in the front and/or rear storage volume to releasably secure objects of different sizes and shapes in the front or rear storage volumes. Hooks may be employed in the front and/or rear storage volumes to allow an object with a strap to be supported in the front or rear storage volume.

Abstract: The disclosed method may include configuring one or more brakes based on a braking-related attribute expected at a geographic location being traversed by a personal mobility vehicle. By configuring the application of brakes of a personal mobility vehicle based on terrain, environmental conditions, and other geographic features, the system may reduce the risk of the vehicle skidding or tipping due to over-braking. In some embodiments, a rider may use a single brake lever to indicate a desire to brake and the system may make determinations about how to apply a combination of mechanical and electrical brakes to front and back wheels based at least in part on the location of the personal mobility vehicle. By configuring brake engagement based on a combination of controls and map data, the system may improve user experience and user safety, especially for inexperienced riders.

Abstract: An example of a flow cell includes a substrate; a first primer set attached to a first region on the substrate, the first primer set including an un-cleavable first primer and a cleavable second primer; and a second primer set attached to a second region on the substrate, the second primer set including a cleavable first primer and an un-cleavable second primer.

Abstract: A micromobility electric vehicle includes a front storage volume and a rear storage volume. The front storage volume may be defined on two sides by an angled footboard portion and a column. The rear storage volume may be defined on two sides by a curved footboard portion and a seat post. Retainers including an elastic member may be employed in the front and/or rear storage volume to releasably secure objects of different sizes and shapes in the front or rear storage volumes. Hooks may be employed in the front and/or rear storage volumes to allow an object with a strap to be supported in the front or rear storage volume.

Abstract: Provided herein include various examples of a flow cell and methods for forming aspects of flow cell. The method may include applying a first adhesive to a substrate. The method may include orienting a die on the first adhesive. The method may also include orienting a package on the first adhesive. The package includes a die and a top surface of the die comprises an active surface and electrical contact points. Surfaces adjacent to the active surface on at least two opposing sides of the active surface form fanout regions for utilization in a fluidic path of the flow cell. The method further may include applying a second adhesive to a part of the package and attaching a lid to the second adhesive to define a fluidic flow-cell cavity below the lid and above a surface comprising the active surface and the fanout regions.

Abstract: A flow cell package includes first and second surface-modified patterned wafers and a spacer layer. The first surface-modified patterned wafer includes first depressions separated by first interstitial regions, a first functionalized molecule bound to a first silane or silane derivative in at least some of the first depressions, and a first primer grafted to the first functionalized molecule in the at least some of the first depressions. The second surface-modified patterned wafer includes second depressions separated by second interstitial regions, a second functionalized molecule bound to a second silane or silane derivative in at least some of the second depressions, and a second primer grafted to the second functionalized molecule in the at least some of the second depressions. The spacer layer bonds at least some first interstitial regions to at least some second interstitial regions, and at least partially defines respective fluidic chambers of the flow cell package.

Abstract: A flow cell includes a support and a heteropolymer attached to the support. The heteropolymer includes an acrylamide monomer including an attachment group to react with a functional group attached to a primer, and a monomer including a stimuli-responsive functional group. The monomer including the stimuli-responsive functional group may be pH-responsive, temperature-responsive, saccharide-responsive, nucleophile-responsive, and/or salt-responsive.