Patents by Inventor Luis Blanco

Luis Blanco has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Methods for amplification and sequencing using thermostable TthPrimPol
    Patent number: 11299718
    Abstract: The present invention is directed to methods for replicating, amplifying, and sequencing of nucleic acids using the thermostable, bifunctional replicase “TthPrimPol” from Thermus thermophilus HB27. The TthPrimPol enzyme is extremely tolerant to alterations of the nucleotides of the template nucleic acid. Therefore, in one aspect the invention discloses methods for replicating, amplifying and sequencing of damaged polynucleotide templates.
    Type: Grant
    Filed: March 14, 2014
    Date of Patent: April 12, 2022
    Assignee: 4BASEBIO SL
    Inventors: Angel J. Picher, Luis Blanco
  • METHODS FOR AMPLIFICATION AND SEQUENCING USING THERMOSTABLE TTHPRIMPOL
    Publication number: 20160040143
    Abstract: The present invention is directed to methods for replicating, amplifying, and sequencing of nucleic acids using the thermostable, bifunctional replicase “TthPrimPol” from Thermus thermophilus HB27. The TthPrimPol enzyme is extremely tolerant to alterations of the nucleotides of the template nucleic acid. Therefore, in one aspect the invention discloses methods for replicating, amplifying and sequencing of damaged polynucleotide templates.
    Type: Application
    Filed: March 14, 2014
    Publication date: February 11, 2016
    Applicant: SYGNIS BIOTECH S.L.U.
    Inventors: Angel J. PICHER, Luis BLANCO
  • .phi.29 DNA polymerase
    Patent number: 5576204
    Abstract: An improved method for determining the nucleotide base sequence of a DNA molecule employs a .phi.-29 type DNA polymerase modified to have reduced or no exonuclease activity. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleotide base, wherein each the agent terminates DNA synthesis at a different nucleotide base; and separating the DNA products of the incubating reaction according to size, whereby at least a part of the nucleotide base sequence of the DNA can be determined. The improvement is provision of a DNA polymerase which is a .phi.29-type DNA polymerase.
    Type: Grant
    Filed: February 11, 1993
    Date of Patent: November 19, 1996
    Assignee: Consejo Superior Investigaciones Cientificas
    Inventors: Luis Blanco, Antonio Bernad, Margarita Salas
  • PHI29 DNA polymerase
    Patent number: 5198543
    Abstract: An improved method for determining the nucleotide base sequence of a DNA molecule. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleotide base, wherein each the agent terminates DNA synthesis at a different nucleotide base; and separating the DNA products of the incubating reaction according to size, whereby at least a part of the nucleotide base sequence of the DNA can be determined. The improvement is provision of a DNA polymerase which is a .phi.29-type DNA polymerase.
    Type: Grant
    Filed: March 13, 1991
    Date of Patent: March 30, 1993
    Assignee: Consejo Superior Investigaciones Cientificas
    Inventors: Luis Blanco, Antonio Bernad, Margarita Salas
  • PH.phi.29 DNA polymerase
    Patent number: 5001050
    Abstract: An improved method for determining the nucleotide base sequence of a DNA molecule. The method includes annealing the DNA molecule with a primer molecule able to hybridize to the DNA molecule; incubating the annealed mixture in a vessel containing four different deoxynucleoside triphosphates, a DNA polymerase, and one or more DNA synthesis terminating agents which terminate DNA synthesis at a specific nucleotide base, wherein each the agent terminates DNA synthesis at a different nucleotide base; and separating the DNA products of the incubating reaction according to size, whereby at least a part of the nucleotide base sequence of the DNA can be determined. The improvement is provision of a DNA-polymerase which is a .phi.29-type DNA polymerase.
    Type: Grant
    Filed: March 24, 1989
    Date of Patent: March 19, 1991
    Assignee: Consejo Superior Investigaciones Cientificas
    Inventors: Luis Blanco, Antonio Bernad, Margarita Salas