Patents by Inventor Luis Branco

Luis Branco has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Compositions and methods for metabolic selection of transfected cells
    Patent number: 8076102
    Abstract: The present invention relates to novel selection marker vectors, and methods for using these vectors to generate stable gene expression systems in eukaryotic cells utilizing any enzyme useful in the eukaryotic sterol/cholesterol biosynthetic pathway, such as a 3-ketosteroid reductase, as a metabolic selection marker to select transfected cells. In one embodiment, the method comprises transfecting cells that are auxotrophic for cholesterol with a vector encoding 3-ketosteroid reductase and at least one heterologous protein, and selecting cells that have the ability to survive in medium lacking cholesterol and/or producing the heterologous protein in these cells in chemically defined and/or serum-free media.
    Type: Grant
    Filed: May 18, 2006
    Date of Patent: December 13, 2011
    Assignee: Biofactura, Inc.
    Inventors: Luis Branco, Darryl Sampey
  • Compositions and Methods for Metabolic Selection of Transfected Cells
    Publication number: 20100028940
    Abstract: The present invention relates to novel selection marker vectors, and methods for using these vectors to generate stable gene expression systems in eukaryotic cells utilizing any enzyme useful in the eukaryotic sterol/cholesterol biosynthetic pathway, such as a 3-ketosteroid reductase, as a metabolic selection marker to select transfected cells. In one embodiment, the method comprises transfecting cells that are auxotrophic for cholesterol with a vector encoding 3-ketosteroid reductase and at least one heterologous protein, and selecting cells that have the ability to survive in medium lacking cholesterol and/or producing the heterologous protein in these cells in chemically defined and/or serum-free media.
    Type: Application
    Filed: May 18, 2006
    Publication date: February 4, 2010
    Applicant: BioFactura, Inc.
    Inventors: Luis Branco, Darryl Sampey