Abstract: Method for the preparation of an enantiomerically enriched acylated amine wherein a phenylacetic acid derivative is brought into contact with a mixture of enantiomers of an amine H2NCR1R2R3, in which R1, R2, R3 are not equal to each other and each independently stand for H, CN, a whether or not substituted (cyclo)alkyl, ary-, alkylaryl or arylalkyl group, whether or not cyclic heteroalkyl or heteroaryl group with one or more N, O or S atoms or in which R1 and R2 (and R3) together with the C-atom to which they have been bound form a (bi)cyclic group that optionally contains one or more N, O of S atoms, in the presence of a Pen-G acylase derived from Alcaligenes faecalis. And method for the preparation of enantiomerically enriched amine of formula H2NCR1R2R3, in which R1, R2, R3 are as defined above, wherein an acylated amine is brought into contact with a Pen-G acylase originating from Alcaligenes faecalis.