Abstract: A bioprocessing system comprising a series of processing stations for performing operations for bioprocessing is disclosed. The bioprocessing system includes an automated system comprising means for manipulating a fluid connection between a first container and a separable second container whereby to create an aseptic connection that enables a controlled transfer of fluid or cell material between the first container and the second container, wherein the means for manipulating a fluid connection is configured to create an aseptic connection that can be disconnected after the transfer of fluid or cell material is complete to enable a further such fluid connection to be manipulated between the first container and a separable third container, and means for controlling an automated sequence of operation of the processing stations.