Abstract: Provided herein are a device for well plate temperature control, a method of independently controlling the temperature in individual wells of a microwell plate, and a temperature-responsive protein. The device includes a microwell plate with at least one well formed therein; a temperature control assembly including a printed circuit board with at least one pair of thermistors extending therefrom, each of the at least one pair of thermistors arranged and disposed to align with one of the at least one wells; and a microcontroller configured to individually control each of the at least one pair of thermistors. The method includes positioning the temperature control assembly adjacent to the well plate such that each pair of thermistors extends into one of the wells, and independently providing a current flow to each of the at least one pair of thermistors to separate heat each well.

Abstract: Provided herein is a stimulation and measurement device. The stimulation and measurement device includes a microwell plate having a top side, a bottom side, and at least one well extending from the top side towards the bottom side; a first assembly removably positioned on the top side of the microwell plate; a second assembly removably positioned on the bottom side of the microwell plate; and a microcontroller. The first assembly includes a first printed circuit board having at least one pair of stimulating light emitting diodes (LEDs) positioned thereon to align with one of the wells. The second assembly includes a second printed circuit board having at least one photodiode positioned thereon to align with one of the pairs of stimulating LEDs, and at least one excitation LED paired with each of the photodiodes. The microcontroller is configured to control the stimulating LED(s), the photodiode(s), and the excitation LED(s).

Abstract: The disclosed apparatus, systems and methods relate to an illumination opto-plate configured to specifically light the wells of a culture plate.