Abstract: The invention relates to a device for the automatic determination of an analyte in the liquid phase by conducting a specific chemical reaction and subsequent optical measurement of the concentration of its products, using spectroscopic techniques, operating in stationary or flow mode. This device is equipped with a reaction-detection system with a replaceable cartridge. The device enables conducting chemical reactions in the liquid phase with the mixing of many streams of reagents, and also ensures the quantitative nature of the determination. The invention also relates to a method for the automatic determination of an analyte in the liquid phase using the device according to the invention, in particular for monitoring the progress of the dialysis process.

Abstract: The present invention is directed to a method of manufacturing a polyepitopic protein comprising the steps of cloning a blunt-ended DNA sequence by encoding the epitope that is to be cloned into a DNA vector recognized by the endonuclease Small or the endonuclease Sapl and isolating the polyepitopic protein by transforming a bacterial host cell with such vector, as well as a DNA vector for embodying this method.

Abstract: We disclose a method of obtaining a polyepitopic protein, a DNA vector for the embodiment of the method as well as polyepitopic proteins fused with macromolecular carriers. The proteins obtained according to the present invention are in a number of uses, in particular when used in the production of improved vaccines.