Abstract: Provided are methods for determining that a candidate polypeptide in a library of polypeptides has editing activity comprising expressing in a plurality of cells: a library of first expression cassettes each comprising a nucleic acid encoding a polypeptide wherein each polypeptide in the library comprises, a first editing domain, a first linker, a nuclease-deficient RNA guided endonuclease domain, a second linker, and a second editing domain; and a second expression cassette comprising a nucleic acid encoding a guide RNA directed to a target of interest, wherein said first and second expression cassettes are expressed in the plurality of cells; separating the plurality of cells based on the expression level of the target of interest; extracting genomic DNA (gDNA) from the plurality of cells; and sequencing the gDNA of cells that have a high level expression or a low level of expression of the target of interest.

Abstract: Provided are compounds for inhibiting prostaglandin E synthase 3 (PTGES3) along with methods of treating prostate cancer by administering such compounds to a subject. The PTGES3 inhibitors has a moiety that can form a covalent bond with a thiol group, for example, of a cysteine residue of PTGES3. By forming such a bond, the inhibitor can block a site on PTGES3 that is involved with the interaction between PTGES3 and androgen receptor (AR). Since the progression of prostate cancer can depend on AR activity, blocking the interaction of PTGES3 and AR can be used to treat prostate cancer.

Abstract: Methods, compositions, and kits are provided for CRISPR/Cas mediated transcriptional modulation.

Abstract: Provided herein are methods directed to multiplexed detection of the modulation of transcriptional activity using perturbation elements.

Abstract: Provided herein are methods directed to multiplexed detection of the modulation of transcriptional activity using perturbation elements.