Abstract: A 3D scaffold (3-dimensional scaffold) is comprised of a biocompatible polymer. The 3D scaffold includes a recess that is open towards the top side of the 3D scaffold as a colonization chamber for biological cells, a canal-type vessel, which at least partially surrounds the colonization chamber, a filling opening for the canal-type vessel, and an outlet opening for the canal-type vessel. A production method for the 3D scaffold is also provided and the 3D scaffold is used for colonizing the colonization chamber with biological cells.

Abstract: A 3D scaffold of a biocompatible polymer and colonized with biological cells is provided., The biological cells can be cultured to form a 3D cell culture construct that closely approximates a physiological architecture. A method for producing the 3D scaffold colonized with biological cells is also provided.

Abstract: A method for producing a cell culture insert with at least one membrane, in particular at least one biological membrane, may include the following steps: providing at least one insert blank with at least one opening; and forming the at least one membrane in the insert blank by means of a bio-printing method.

Abstract: A method for producing a three-dimensional, multi-cell object, in which polymerized structures are produced in layers by the irradiation of light radiation. To this end, use is made of photopolymerizable liquids which at least partly comprise biological cells. The invention also relates to a device for carrying out such a method and to the use of this device for producing an artificial organ.

Abstract: A method for producing a three-dimensional, multi-cell object, in which polymerized structures are produced in layers by the irradiation of light radiation. To this end, use is made of photopolymerizable liquids which at least partly comprise biological cells. The invention also relates to a device for carrying out such a method and to the use of this device for producing an artificial organ.

Abstract: A method for at least one of determining and monitoring at least one condition of/in a three dimensional cell culture system comprising at least one growth section, the at least one condition being selected from the group consisting of a physiological condition, a vitality, and a metabolism status, the method includes determining the physiological condition using erythrocytes as detectors, determining and/or monitoring the vitality by measuring living cell fluorescent dyes, and determining and/or monitoring the metabolism status by at least one of measuring an autofluorescence of NADH and/or FAD, by determining a NADH/NAD+ ratio, and by determining a NADH/FAD ratio.