Abstract: A biologic and medical multi-channel low voltage micro-electric-field generator including a power supply unit, at least one micro-electric-field generating unit set comprising a step-down unit, a linear regulator unit, and a pulse generating and outputting unit, and a programmable logic control unit. The micro-electric-field generating unit set is connected to an output end of the power supply unit. The step-down unit depresses the voltage of frequency power. The linear regulator unit regulates the output of the step-down unit. The pulse generating and outputting unit turns on/off the output of the linear regulator unit and connects to a network electrode group. The programmable logic control unit controls the characteristics of the output pulse from the pulse generating and outputting unit. The generator is applicable in gene, protein, drug and/or a variety of plasmids delivery to the organs, cells within the tissues of large animal or human.

Abstract: An intelligent remote-controlled portable intravenous injection and transfusion system includes a fluid storage unit (A), a transfusion needle (B), a transfusion tube (C) and a fluid supply driving unit (D). The fluid storage unit (A) is formed by a portable bag (A1) and a fluid storage bag (A2) therein. The fluid supply driving unit (D) includes a peristaltic pump (2), which is provided in a portable flip-shell formed by a lower case (5), an upper cover (6) and a rotational axis (7), and its drive device (1), a power (13), a controller having MSF module and the second wireless signal transmitter/receiver unit. The second wireless signal transmitter/receiver unit is connected to the I/O of the controller. The first wireless signal transmitter/receiver unit is provided in a healthcare monitor room, and connected to the I/O of a computer installed with a controlling software.

Abstract: A safety transfusion catheter includes a flexible retention tube fitted over a metal guiding needle. The flexible retention tube is provided with a needle handle connected with it. The angle between the end of the flexible retention tube and its longitudinal axis or longitudinal tube wall is less than 90.degree. The cross section of the needle handle has an outer contour line of a closed curve formed with a curve intersecting a beeline. A further safety transfusion catheter includes a flexible retention tube fitted over a metal guiding needle, a transfusion connecting tube in fluid communication with flexible retention tube and a rubber casing cap at the end of the flexible retention tube. The transfusion connecting tube and flexible retention tube form a T-shaped or an oblique Y-shaped structure.

Abstract: A drug delivery apparatus guided by micro electrical field network for target cells of liver comprises a low-voltage micro- electrical field generator and a drug delivery catheter which gets into a liver through an endoscope or indwelling catheter along blood vessels, wherein at least two drug infusion holes and a first array of electrodes are provided at the front end of the drug delivery catheter; a second array of electrodes is set on the exterior of the liver; the first array of electrodes and the second array of electrodes are connected with the pulse output terminal of the low-voltage micro-electrical field generator; the drug delivery catheter is connected through with a drug transportation device.

Abstract: A multipurpose micro electric field network cell processing device comprises: a hollow frame structure; transparent film layers which are respectively correspondingly arranged on the upper side surface and the lower side surface of the frame; electrode groups which are arranged on the transparent film layer on at least one side and used for generating a low strength electric field; wire connecting terminals of the electrode groups arranged on at least one end of the frame; and an inlet and an outlet of culture/processing solution arranged at two corresponding ends of the frame, wherein sandwich space surrounded by the two transparent film layers and the frame constitutes a cell culture/processing chamber. The device can effectively introduce or deliver a drug into target cells under a low strength electric field network environment, monitor and control the whole experiment/processing process more precisely and be conducive to separation and selection of the target cells.

Abstract: A biologic and medical multi-channel low voltage micro-electric-field generator including a power supply unit, at least one micro-electric-field generating unit set comprising a step-down unit, a linear regulator unit, and a pulse generating and outputting unit, and a programmable logic control unit. The micro-electric-field generating unit set is connected to an output end of the power supply unit. The step-down unit depresses the voltage of frequency power. The linear regulator unit regulates the output of the step-down unit. The pulse generating and outputting unit turns on/off the output of the linear regulator unit and connects to a network electrode group. The programmable logic control unit controls the characteristics of the output pulse from the pulse generating and outputting unit. The generator is applicable in gene, protein, drug and/or a variety of plasmids delivery to the organs, cells within the tissues of large animal or human.

Abstract: A methodology and apparatus for drug, gene, siRNA, shRNA shRNA peptide, protein, antibody or any other biomedical therapeutic reagents targeting in various of solid organs of large animals and humans ex vivo and in vivo is described. The delivery is assisted with the application of a low strength electric field network (LSEN). A low strength electric field network (LSEN) system is used for ex vivo or in vivo delivery of any therapeutic gene, siRNA, shRNA shRNA protein or drugs into a lung, pleura, breast, liver, spleen, pancreas, kidney, adrenal, prostate, testicle, ovary and tumors in chest, abdominal and pelvic cavity.

Abstract: Application to an allograft or xenograft of a low strength electric field network (LSEN) together with an immuno-suppressive drug, gene and siRNA or other gene-based therapy is used to mediate the immune responses within an donor organ, tissue or cells, to prevent the acute and chronic rejection and to induce true tolerance, The gene(s) is locally transferred ex vivo in the time interval between harvest and implantation of allografts or xenografts before the implantation to introduce the long-term over expression of immunosuppressive and/or modulative molecules, or for down regulating alloreactive molecules in the donor organ, tissue or cells only and not in the recipient's whole body system.

Abstract: A non-electrode-lead ultra-thin flexible micro multifunctional heart rate adjusting device comprises an integrative ultra-thin flexible micro non-electrode-lead pacemaker formed by assembling a micro battery, an ultra-low-power source circuit, a wireless receiving/transmitting circuit and an application circuit unit together, the needle electrodes are positioned on one side of the pacemaker and all of them form a small electrode body which can directly implanted into heart or external surface of heart; a multifunctional microcomputer heart rate adjusting remote controller connects with various non-electrode-lead pacemakers via wireless communication; the non-electrode-lead pacemakers and/or the heart rate adjusting remote controller connect with a control base station via wireless network, and the control base station connects with a computer.

Abstract: An electromagnetic field is applied to transplanted cells during their transplantation culturing to achieve confluence and alignment of transplanted cells into damaged tissue and organs, therefore improving the synchronization of electrical and mechanical tissue or organ functions. The electromagnetic field may be applied by catheter based devices to a selected portion of tissue or to the entire surface of a hollow body cavity or organ, such as a heart chamber. The electromagnetic field is provided by an expansible net or array from a catheter or on an inflatable balloon or stent. The electromagnetic field is also provided by an implantable net or array of electrodes which may either be hardwired to a pulse generator or coupled by means of wireless or inductive transmission.

Abstract: A safety transfusion catheter includes a flexible retention tube (2) fitted over a metal guiding needle (1). The flexible retention tube (2) is provided with a needle handle (22; 22?) connected with it. The angle between the end (3) of the flexible retention tube (2) and its longitudinal axis or longitudinal tube wall is less than 90°. The cross section of the needle handle (22; 22?) has an outer contour line of a closed curve formed with a curve (5, 25) intersecting a beeline (6; 26). A further safety transfusion catheter includes a flexible retention tube (39) fitted over a metal guiding needle (32), a transfusion connecting tube (38) in fluid communication with flexible retention tube (39) and a rubber casing cap (42) at the end of the flexible retention tube (39). The transfusion connecting tube (38) and flexible retention tube (39) form a T-shaped or an oblique Y-shaped structure.

Abstract: An intelligent remote-controlled portable intravenous injection and transfusion system includes a fluid storage unit (A), a transfusion needle (B), a transfusion tube (C) and a fluid supply driving unit (D). The fluid storage unit (A) is formed by a portable bag (A1) and a fluid storage bag (A2) therein. The fluid supply driving unit (D) includes a peristaltic pump (2), which is provided in a portable flip-shell formed by a lower case (5), an upper cover (6) and a rotational axis (7), and its drive device (1), a power (13), a controller having MSF module and the second wireless signal transmitter/receiver unit. The second wireless signal transmitter/receiver unit is connected to the I/O of the controller. The first wireless signal transmitter/receiver unit is provided in a healthcare monitor room, and connected to the I/O of a computer installed with a controlling software.

Abstract: An apparatus for in vivo intracellular transfection of gene, siRNA, shRNA vectors, and other biomedical diagnostic and therapeutic drugs and molecules for the treatment of arthritis and other orthopedic diseases in large animals and humans includes: a source of low voltage, short duration pulses in long duration bursts (LSEN); an electrode mesh system coupled to the source for generating distributed electric field network into a joint, including bones, cartilages, and related tissues; and means for transfecting the gene, siRNA, shRNA vectors, and other biomedical diagnostic and therapeutic drugs and molecules into a joint. The electrode mesh system includes alternatively arranged negative and positive electrodes in a first array which is capable of being inserted into a joint cavity, and either an alternatively arranged negative and positive or an all negative second electrode array which is positioned outside of the joint and in directly contact with overlying skin.

Abstract: The illustrated embodiments of the invention include four preferred embodiments: 1) a method and apparatus for the joint and its related soft tissue for bone gene, protein and drug delivery; 2) a method and apparatus for gene, protein and drug delivery to an extremity; 3) a method and apparatus for delivery of gene, protein and drug delivery to skin and soft tissue; and/or 4) a method and apparatus for delivery of a gene, protein and drug to soft tissue tumor.

Abstract: An composition in a method for noninvasively monitoring the expression of therapeutic transgene delivered ex vivo and in vivo for the treatment of diseases includes the step of quantitatively imaging a reporter gene expression which is coupled to a therapeutic gene on a plasmid vector to infer levels, location, or duration of the therapeutic gene expression in the targeted tissues or organs. The reporter gene is imaged using a radiopharmaceutical for scintigraphic imaging of the gene expression interactions with the reporter gene, namely positron emission tomography, gamma camera or single-photon emission computed tomography. The genes are delivered with a liposome encapsulated reporter-therapeutic linked transgene vector with balanced reporter/therapeutic transgene expression. A transgene composition includes the reporter gene linked to the therapeutic gene or genes incorporated in and delivered by a liposome encapsulated reporter-therapeutic linked transgene vector.

Abstract: Ex vivo gene, protein or drug delivery to macroscopic quantities of various types of cells, cell clusters, or tissues using ultra low strength LSEFN strategies is disclosed in which the bioengineered cells and tissues are then systemically transfused, delivered or implanted into the various organs or tissue for the treatment of diseases. An LSEFN chamber is used which is shaped and sized to intimately contain the cells, cell clusters, or tissues in a transfusion chamber between opposing membrane encapsulated electrode arrays across which LSEFN pulses are applied.

Abstract: An electromagnetic field is applied to transplanted cells during their transplantation culturing to a chi eve confluence and alignment of transplanted cells into damaged tissue and organs, therefore improving the synchronization of electrical and mechanical tissue or organ functions. The electromagnetic field may be applied by catheter based to a selected portion of tissue or to the entire surface of a hollow body cavity or organ, such as heart chamber. The electromagnetic field is provided by an expansible net or array from a catheter or on an inflatable balloon or stent. The electromagnetic field is also provided by an implantable net or array of electrodes which may either be hardwired to a pulse generator or coupled by means of wireless or inductive transmission.

Abstract: A catheter-delivered electrode array is used to three dimensionally map the endomyocardial monophasic action potential (MAP). The catheter is used in a method for endomyocardial MAP mapping and for establishing diagnostic criteria for the cardiac myocyte injury. During allograft rejection, conduction impairment occurs within the atrial myocardium. Abnormalities of the terminal force of the P wave in Lead V1 (ptfV1) and dispersion of corrected atrial repolarization (Ta-TcD) represent interatrial conduction defects that accompanies the rejection process. Three dimensional endomyocardium monophasic action potential mappings are used to directly monitor the pathophysiological changes in cardiac myocytes. Changes in amplitude, duration and morphology of the action potential were recorded and used to detect the early rejection of the transplant organ with high sensitivity and specificity.

Abstract: An array is placed in contact with or close proximity with an organ or tissue surface to cause electroporation for gene, protein, drug delivery in both ex vivo and in vivo applications. A low DC voltage with a short pulse duration, and long burst pulse is applied to the array. A long rest period is provided between pulse bursts to allow for cell recovery. To enable the application of a low voltage shock in a large organ with same transfection efficiency, four gene, protein and drug delivery systems are illustrated.

Abstract: A genetic mutation within the SR calcium release channel provides a test for susceptibility to idiopathic dilated cardiomyopathy. The test detects the presence of the mutation in a sample of nucleic acids obtained from the individual being tested. Restriction fragment length polymorphism is one technique which can be used in the test.