Abstract: A microfluidic chip device based on a magnetic field-controlled fluorescently labeled cell sorting method and a use method are disclosed. The microfluidic chip comprises a sample channel, two sheath fluid channels, a first fluorescence detection area, a second fluorescence detection area, a magnetic field control system, a magnetic field-controlled cell sorting area, a target cell channel, and a waste fluid channel. Based on fluorescence signals labeled on the cells, the FACS system is combined with a magnetic field-controlled sorting system to achieve automated cell sorting, which not only miniaturizes and automates the sorting detection integration, but also has the advantages of easy and economical operation. The device sorts both a large number of cell samples and a small number of cell samples, thus overcoming the defects of a flow cytometer.