Patents by Inventor Lydia Contreras-Martinez

Lydia Contreras-Martinez has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Protein discovery using intracellular ribosome display
    Patent number: 9879252
    Abstract: The present invention relates to a method of identifying a protein that binds to a target molecule and has intracellular functionality. This method includes providing a construct comprising a deoxyribonucleic acid molecule encoding the protein which binds to the target molecule, with the deoxyribonucleic acid molecule being coupled to a stall sequence. A host cell is transformed with the construct and then cultured under conditions effective to form, within the host cell, a complex of the protein whose translation has been stalled, the mRNA encoding the protein, and ribosomes. The protein in the complex is in a properly folded, active form and the complex is recovered from the cell.
    Type: Grant
    Filed: July 31, 2008
    Date of Patent: January 30, 2018
    Assignee: Cornell Research Foundation, Inc.
    Inventors: Matthew P. Delisa, Lydia Contreras-Martinez
  • PROTEIN DISCOVERY USING INTRACELLULAR RIBOSOME DISPLAY
    Publication number: 20110008774
    Abstract: The present invention relates to a method of identifying a protein that binds to a target molecule and has intracellular functionality. This method includes providing a construct comprising a deoxyribonucleic acid molecule encoding the protein which binds to the target molecule, with the deoxyribonucleic acid molecule being coupled to a stall sequence. A host cell is transformed with the construct and then cultured under conditions effective to form, within the host cell, a complex of the protein whose translation has been stalled, the mRNA encoding the protein, and ribosomes. The protein in the complex is in a properly folded, active form and the complex is recovered from the cell. This method can be carried out with a cell-free extract preparation containing ribosomes instead of a host cell.
    Type: Application
    Filed: July 31, 2008
    Publication date: January 13, 2011
    Applicant: CORNELL RESEARCH FOUNDATION INC
    Inventors: Matthew P. Delisa, Lydia Contreras-Martinez