Abstract: Lipid bilayer particles, such as extracellular vesicles (EVs), are labeled with lipid-tagged single-stranded DNA (ssDNA), which is amplified by polymerase chain reaction (PCR) to quantify particles captured via surface marker interactions or complementary oligonucleotide hybridization.

Abstract: Mechano-node-pore sensing (mechano-NPS), is a rapid, multi-parametric cell screening method that simultaneously quantifies cell diameter, transit time through a contraction channel, transverse deformation under constant strain, and recovery time after deformation.

Abstract: Mechano-node-pore sensing (mechano-NPS), is a rapid, multi-parametric cell screening method that simultaneously quantifies cell diameter, transit time through a contraction channel, transverse deformation under constant strain, and recovery time after deformation

Abstract: Devices for sorting cells in a fluid sample are provided. The device includes a microfluidic conduit configured to carry a flow of a fluid sample. The conduit includes a longitudinal divider extending from an interior surface of the conduit towards an opposing interior surface of the conduit, where the longitudinal divider defines a first longitudinal fluid flow path in fluid communication with a second longitudinal fluid flow path in the conduit. Also provided are methods of using the devices as well as systems and kits that include the devices. The devices, systems and methods find use in a variety of different applications, including diagnostic assays.

Abstract: A quantitative microchip Coulter counter is provided for use in detecting and measuring particles or macromolecules. The device comprises a conduit, a fluid handling system, and a measurement system. Preferably, the conduit is at least in part formed from an elastomeric material.

Abstract: A coplanar waveguide for use in dielectric spectroscopy of biological solution is described. The waveguide's inner conductor can have a small gap and a sample containing space is laid over the gap. The sample containing space holds a small volume, ranging from a few picoliters to a few microliters of a biological solution. The waveguide is then driven with electrical signals across an extremely wide frequency range from 40 Hz to 40 GHz. The waveguide is coupled to a network or impedance analyzer by means of appropriate connectors and the response of the biological solution to the input signals is recorded. One-port and two-port measurements can be made without any modifications. The simple geometry of the waveguide makes it easy to integrate with microfluidic systems.

Abstract: A coplanar waveguide for use in dielectric spectroscopy of biological solution is described. The waveguide's inner conductor can have a small gap and a sample containing space is laid over the gap. The sample containing space holds a small volume, ranging from a few picoliters to a few microliters of a biological solution. The waveguide is then driven with electrical signals across an extremely wide frequency range from 40 Hz to 40 GHz. The waveguide is coupled to a network or impedance analyzer by means of appropriate connectors and the response of the biological solution to the input signals is recorded. One-port and two-port measurements can be made without any modifications. The simple geometry of the waveguide makes it easy to integrate with microfluidic systems.

Abstract: A quantitative microchip Coulter counter is provided for use in detecting and measuring particles or macromolecules. The device comprises a conduit, a fluid handling system, and a measurement system. Preferably, the conduit is at least in part formed from an elastomeric material.