Abstract: Disclosed herein are compositions, devices, and methods that provide cellular materials in ready to use formats for experimental, therapeutic, and tissue engineering protocols. For example, compositions containing frozen or non-frozen cell present as aggregates are disclosed. Also disclosed a compositions containing cellular materials that after storage are suitable for dispersion, e.g. by 3D printing. Also disclosed is a kit for producing bioink compositions. Also disclosed is a closed system device comprising a cell material composition described herein.

Abstract: Disclosed herein are compositions, kits, and methods involving hypertonic solutions. Specifically, disclosed herein are methods and compositions used for increasing the yield of extracellular material from cells. This occurs when said cells are exposed to hypertonic solutions.

Abstract: Apparatus and corresponding method for concentration and washing of live mammalian cells, for preparation of human cell therapy products. Optimized parameters for a temperature regulated, completely closed, fully disposable and scalable counterflow centrifugation separation system having integrated disposables designed for both the input cells and output cells are provided.

Abstract: A multiport container system for use in cell culture processes in the field of regenerative medicine is disclosed. Specifically, the container system can have a port that connects to a fill tubing for media and reagent addition, and additional ports for tubing and connectors which are compatible to various types of cell therapy bioreactors, single use bags, as well as different sampling and cell culture systems. The multiport container system can be used for cell production processes where a single bag could be used to fill, transport, and store media or reagents, as well as to seed and feed cells into different types of culture platform. The disclosed multiport container system can be used for the streamlining of process work flow which enable rapid process development and process transfer into cell product manufacturing.

Abstract: Disclosed herein are compositions and methods involving stem cells biopreserved on microcarriers, which can be thawed and expanded, all while maintaining their key attributes, such as their proliferative capacity, identity, functionality, and potency. Disclosed is a method for generating microcarriers-seeded-stem cells, as well as a post biopreservation procedure for thawing and inoculating bioreactors to achieve a rapid and scalable stem cells expansion.

Abstract: Disclosed herein are compositions, devices, and methods that provide cellular materials in ready to use formats for experimental, therapeutic, and tissue engineering protocols. For example, compositions containing frozen or non-frozen cell present as aggregates are disclosed. Also disclosed a compositions containing cellular materials that after storage are suitable for dispersion, e.g. by 3D printing. Also disclosed is a kit for producing bioink compositions. Also disclosed is a closed system device comprising a cell material composition described herein.

Abstract: Apparatus and corresponding method for concentration and washing of live mammalian cells, for preparation of human cell therapy products. Optimized parameters for a temperature regulated, completely closed, fully disposable and scalable counterflow centrifugation separation system having integrated disposables designed for both the input cells and output cells are provided.