Abstract: The present invention relates to methods for obtaining a substantially pure population of pluripotent stem cell-derived airway basal-like cells. It also relates to a method of obtaining an in vitro pluripotent stem cell-derived airway epithelium model, utilising the pluripotent stem cell-derived airway basal-like cells. The invention further relates to an in vitro airway epithelial model, or lung model, which can be used for disease modelling and/or drug screening and in particular to an in vitro model for SARS-CoV-2 infection and for screening for agents effective against infection with SARS-CoV-2 i.e. COVID-19 and methods of using the same.

Abstract: The present invention relates to methods for making in vitro retinal cultures, tissue, or retinal organoids, from pluripotent cells as well as the improved synthetic retinal tissue and retinal organoids themselves. It also relates to retinal organoids that replicate in vitro many characteristics of the retina (e.g., human or mammalian), and methods of using this retinal organoid to study disease and to identify therapeutic agents for the treatment of retinal diseases and disorders.

Abstract: An alizarin-based chromogenic substrate, a composition containing the substrate, and its use to detect the presence of an enzymatic activity are disclosed. The substrate is particularly applicable in the field of biological diagnosis.

Abstract: An alizarin-based chromogenic substrate, a composition containing the substrate, and its use to detect the presence of an enzymatic activity. The substrate is particularly applicable in the field of biological diagnosis.

Abstract: An alizarin-based chromogenic substrate, a composition containing the substrate, and its use to detect the presence of an enzymatic activity are disclosed. The substrate is particularly applicable in the field of biological diagnosis.

Abstract: The invention concerns novel enzymatic substrates of general formula (I) wherein: X represents a nitrogen atom or a carbon atom substituted with a phenyl group, said phenyl group being optionally substituted in meta or para position: Y and Z represent a hydrogen atom when X represents a carbon atom or Y and Z represent together an ether, thioether or amine bond optionally substituted with an alkyl or aryl group; R1 and R2 independently represent each H, Cl, F, I, Br and can be identical or different or R1 and R2 together represent a substituted or non-substituted fused benzene ring, R3 and R4 independently represent each H, Cl, F, I, Br and can be identical or different; R represents a group such that the O—R bond is capable of being hydrolysed by an enzyme. The invention also concerns a method for synthesizing said substrates, a composition, a kit and a method for detecting at least one micro-organism using said enzymatic substrate.

Abstract: A method and compound for detecting and identifying and/or quantifying a deaminase enzymatic activity of a microorganism, according to which an inoculum suspected of containing a microorganism with a deaminase activity is brought into contact with a culture medium for microorganisms, wherein the culture medium comprises at least one detection agent for demonstrating, by forming a colored product with a revealing agent, a deaminase enzymatic activity; said detection agent being an L-amino acid of following general formula (I): in which R represents an organic radical containing a cyclic ring, said cyclic ring being substituted with 1 to 3 substituents that are identical or different and each of which limits the diffusion in the culture medium of the &agr;-keto acid produced by the deamination of the at least one detection agent, as compared to where each of said substituents is not present.

Abstract: The invention concerns a novel chromogenic substrate based on Alizarin. The invention also concerns the uses to which this substrate can be put, and a formulation containing such a substrate.

Abstract: The invention concerns a chromogenous substrate for detecting the presence of at least one enzyme enzymatic activity. The invention also concerns a method for using such a substrate. The invention further concerns a method for identification based on such substrates and a device for implementing said identification method. The invention is characterized in that the substrate consists of at lease two molecules, a first molecule consisting of a non-chromogenous marker part associated with at least a specific target part for the enzyme and a second molecule consisting of a non-chromogenous part, and the non-chromogenous marker part, once it is released, reacts with the second molecule to form a chromogenous molecule. The invention is particularly applicable in bacteriology.

Abstract: The invention concerns novel 6- or 7-substituted derivatives of esculetin (6,7-dihydroxy-2H-1-benzopyran-2-one), their synthesis, and their application as substrates for the detection of micro-organisms in samples where a derivative is enzymatically cleaved to release a colored or fluorogenic marker which has a low tendency to diffuse through agar or other aqueous environments.

Abstract: The invention concerns novel 6- or 7-substituted derivatives of esculetin (6,7-dihydroxy-2H-1-benzopyran-2-one), their synthesis, and their application as substrates for the detection of micro-organisms in samples where a derivative is enzymatically cleaved to release a colored or fluorogenic marker which has a low tendency to diffuse through agar or other aqueous environments.