Abstract: To sequence long strands of DNA, cloned strands having lengths longer than 100 bases are, in one embodiment, marked on one end with biotih. These strands are divided into 4 aliquots and each aliquot: (1) is uniquely chemically treated to randomly terminate the strands at the non-biotinylated end at a selected type of base; and (2) is moved continuously by electrophoresis through a different one of four identical channels. In the one embodiment, the strands are randomly terminated at a selected base type and they are moved into avidin, which due to high affinity, combines with the biotin marked ends of shorter strands before the longer strands are fully resolved in the gel. The avidin is marked with fluorescein, the strands are scanned and the signals are decoded. In another embodiment, the strands are synthesized, with termination at a selected base type and marked either by the above method or by ethidium bromide.

Abstract: To sequence DNA automatically, DNA marked with near infrared fluorescent dyes are electrophoresed in a plurality of channels through a gel electrophoresis slab wherein the DNA samples are resolved in accordance with the size of DNA fragments in the gel electrophoresis slab into fluorescently marked DNA bands. The separated samples are scanned photoelectrically with a laser diode and a sensor, wherein the laser scans with scanning light at a scanning light frequency within the absorbance spectrum of said fluorescently marked DNA samples and light is sensed at the emission frequency of the marked DNA. The light is modulated from said laser at a predetermined modulation frequency and fluorescent light emitted by said DNA bands at said modulation frequency is detected, whereby background noise from the medium through which the light is transmitted is discriminated against.

Abstract: To sequence DNA automatically, flourescently marked DNA are electrophoresed in a plurality of channels through a gel electrophoresis slab; wherein the DNA samples are resolved in accordance with the size of DNA fragments in the gel electrophoresis slab into fluorescently marked DNA bands. The separated samples are scanned photoelectrically with a laser and a sensor, wherein the laser scans with scanning light at a scanning light frequency within the absorbance spectrum of said fluorescently marked DNA samples and light is sensed at the emission frequency of the marked DNA. The light is modulated from said laser at a predetermined modulation frequency and fluorescent light emitted by said DNA bands at said modulation frequency is detected, whereby background noise from the medium through which the light is transmitted is discriminated against.

Abstract: To provide sufficient sensitivity, spectral resolution and speed of measurement for field environmental measurements in a portable spectroradiometer, a silicon photodiode receives light: (1) having a bandwidth in the range of between 2 and 15 nm (nanometers) from a pivotable concave holographic diffraction grating within the wavelength range of between 250 and 1150 nm at a scanning rate in the range of 20 to 100 nm per second; (2) having stray light of high intensity and undesired frequencies and the shorter wavelength harmonics of the selected frequency range blocked by filters; and (3) having flux of at least 10 microwatts per square meter of diffuser plate for each nanometer of bandwidth. Automatic electrical zeroing is obtained by blocking all light once at the beginning of each scan, obtaining an electrical drift-related signal and using the drift signal to adjust the measured signal during the scan.

Abstract: To sequence long strands of DNA, cloned strands having lengths longer than 100 bases are, in one embodiment, marked on one end with biotin. These strands are divided into 4 aliquots and each aliquot: (1) is uniquely chemically treated to randomly terminate the strands at the non-biotinylated end at a selected type of base; and (2) is moved continuously by electrophoresis through a different one of four identical channels. In the one embodiment, the strands are randomly terminated at a selected base type and they are moved into avidin, which due to high affinity, combines with the biotin marked ends of shorter strands before the longer strands are fully resolved in the gel. The avidin is marked with fluorescein, the strands are scanned and the signals are decoded. In another embodiment, the strands are synthesized, with termination at a selected base type and marked either by the above method or by ethidium bromide.

Abstract: The resolution of the measuring system of a conveyor type leaf-area meter is optimized when changes are made in the size of the area that is scanned by adjusting: (1) the lens of the scanner; (2) the rate of readout of the pulses from the scanner; and (3) the decimal place of the display unit.

Abstract: To synchronize the belt of a conveyor, a scanner, a fluorescent lamp, and an LED display unit of an area meter, the main AC power supply of the area meter drives the conveyor belt through a synchronous motor and supplies the same frequency of power to a phase locked loop. The phase locked loop provides synchronous pulses of higher frequencies to the scanning system, fluorescent lamp and LED display unit to maintain synchronism. The resolution of the measuring system is optimized when changes are made in the size of the area that is scanned by adjusting: (1) the lens of the scanner; (2) the rate of readout of the pulses from the scanner; and (3) the decimal place of the display unit. Travel from side to side of the belt is avoided by individually adjusting the ends of the pulleys until the belt runs true and an idler which lifts both bottom and top run of the belt when an object moves under it prevents slippage of the belt with respect to the rollers and the bite of the conveyor is adjustable.

Abstract: To permit a portable light meter to operate without zero adjustment and without excessive battery drain, a chopper-stabilized amplifier amplifies the DC signal from the light sensor of the light meter. The chopper in the chopper-stabilized amplifier is an FET circuit having a high impedance compensating circuit to avoid battery drain while compensating for interelectrode capacitance. The power supply obtains the ground level potential from the output of an operational amplifier with negative and positive potentials being generated from that reference potential so as to not require a center tap on the battery pack.

Abstract: To provide a printout corresponding to the integral of the current or voltage from any of several different instruments, a printing integrator includes a voltage-to-frequency converter which receives current or voltage through a replaceable buffer amplifier and provides pulses to a counter at a rate proportional to the amplitude of the input current or voltage. The output of the counter is printed in rows along with the time of printing and an indication of the state of the battery.To generate pulses at a rate proportional to an input signal, a charge is periodically supplied to a feedback capacitor of an operational amplifier from a storage capacitor in the voltage-to-frequency converter, and an input current derived from the signal decreases the charge in the feedback capacitor until the output potential of the operational amplifier drops below a level set on a comparator, thus generating a pulse and initiating another discharge from the storage capacitor into the feedback capacitor.