Abstract: The inventors have discovered sets of proteinaceous biomarkers which can be measured in biological fluid samples to diagnosis or aid in the diagnosis of Alzheimer's disease and distinguish AD samples from non-demented samples.

Abstract: The embodiments described herein relate to systems and methods for producing an evaporation barrier in a PCR vial. In some embodiments, beads with a particular distribution in diameters can be used to produce a barrier for reducing the evaporation of liquid PCR samples within the PCR vial. In some embodiments, the beads can be pre-filled in the PCR vial. In use, liquid samples and/or liquid reagents can be introduced in the PCR vial pre-filled with the beads, such that the beads can be driven to the surface of the liquid PCR sample through the buoyancy of the beads.

Abstract: The present invention provides methods and kits for conducting multiplex nucleic acid amplification reactions by controlling target sequence replication times. In one aspect, such control is exerted by selecting different lengths of target polynucleotides for amplification. In another aspect, control is exerted by providing sequence-specific polymerase inhibitors, such as specific blocking oligonucleotides. In accordance with the invention, multiple target polynucleotides can be sequentially amplified in an amplification reaction conducted in different stages, wherein amplification of sequences with longer replication times is permitted in one stage but precluded in other stages by modifying polymerase extension times in the course of the reaction.

Abstract: The inventors have discovered sets of proteinaceous biomarkers which can be measured in biological fluid samples to diagnosis or aid in the diagnosis of Alzheimer's disease and distinguish AD samples from non-demented samples.

Abstract: The present invention provides methods and kits for conducting multiplex nucleic acid amplification reactions by controlling target sequence replication times. In one aspect, such control is exerted by selecting different lengths of target polynucleotides for amplification. In another aspect, control is exerted by providing sequence-specific polymerase inhibitors, such as specific blocking oligonucleotides. In accordance with the invention, multiple target polynucleotides can be sequentially amplified in an amplification reaction conducted in different stages, wherein amplification of sequences with longer replication times is permitted in one stage but precluded in other stages by modifying polymerase extension times in the course of the reaction.