Abstract: Described herein is a cell tissue gel cross-linked with a cross-linking agent, and a quenching agent bound to a reactive group of the cross-linking agent.

Abstract: Use of hyaluronan, either alone or in combination with collagen, for promoting angiogenesis.

Abstract: A method for sterilizing biological materials is disclosed. After dehydrating the biological material, the dehydrated biological material is put into a closed container, and 0.01 g/m3 to 1000 g/m3 or an enough dose of ozone gas is introduced into the container for a period of time until the biological material is completely sterilized. Afterward, the ozone gas is removed from the closed container to finish sterilization of the biological material.

Abstract: A method of preparing porous collagen matrices directly from connective tissues using an acidic solution substantially free of salt.

Abstract: A cell tissue gel containing collagen and hyaluronan at a weight ratio of 0.01-100:1 and uses thereof for stem cell delivery.

Abstract: A method of preparing collagen by first producing a collagen matrix and then extracting collagen from the matrix.

Abstract: A method of preparing collagen by first producing a collagen matrix and then extracting collagen from the matrix.

Abstract: A method of preparing porous collagen matrices directly from connective tissues using an acidic solution substantially free of salt.

Abstract: A method for regulating proliferation of cells, has steps of providing a first culture system with a surface that is coated with a biological material; inoculating and culturing cells on the surface of the first culture system in an appropriate medium, such that the proliferation of the cells is preserved; collecting the cells; providing a second culture system with a surface; and inoculating and culturing the cells on the surface of the second culture system in a culture medium containing the biological material, such that the proliferation of the cells is promoted. A method for regulating proliferation of cells is also provided, the method being the same as the previous method except that the step of inoculating and culturing in a first culture system is performed before the step of inoculating and culturing in a second culture system.

Abstract: A method for preserving proliferation and differentiation potential of undifferentiated cells, has steps of providing a culture carrier having a surface coated with a biological material selected from the group consisting of polysaccharide, sulfated polysaccharide and derivatives thereof; and inoculating and culturing the undifferentiated cells on the surface in the culture carrier with an appropriate medium, such that the proliferation and differentiation potential of undifferentiated cells are preserved. The method can be used for expanding stem cells in vitro without loss of their replicative ability and differentiation capacity. Therefore, the method according to the present invention is amenable to application in regenerative medicine, tissue engineering, and therapy using umbilical cord blood and other cell sources such as peripheral blood, stem cells, tissue progenitor cells, and tissue cells.

Abstract: The subject invention provides a process for preparing a porous collagen matrix from connective tissue, said process comprising: a porous structure forming step to treat said connective tissue with poring agent in situ; and a washing step to remove the impurity from said porous connective tissue thereby obtaining a porous collagen matrix.

Abstract: The subject invention discloses a process for the preparation of a porous collagen matrix. Said process comprises providing a neutral or nearly neutral collagen solution, incubating the collagen solution at a temperature of between about 30 and about 45° C. for a period of time sufficient to reconstitute collagen fibrils to obtain a collagen gel matrix, freezing said collagen gel matrix with an appropriate temperature reduction rate to an appropriate freezing temperature, lyophilizing said matrix to form a porous collagen matrix and treating the lyophilized collagen matrix with an organic solvent that can quickly penetrate into the collagen matrix to prevent the shrinkage thereof.

Abstract: The subject invention provides a process for preparing a porous collagen matrix from connective tissue, which comprises treating connective tissue with a solution of hydrogen peroxide and/or an acidic solution so as to obtain a porous collagen matrix.