Abstract: Described herein are methods for the treatment of hypoxia-associated tissue damage in subjects. More particularly, the methods relate to treating hypoxia-associated tissue damage in a subject during a surgical procedure with a therapeutically effective amount of a glutamine source comprising glutamine, a dipeptide comprising glutamine, or a glutamine amino ester conjugate according to particular dosing regimens. Specifically, the methods concern a dosing scheme comprising dosing a subject during a surgical procedure with a glutamine source wherein the first dose is administered to the subject within about 30 minutes following an initial surgical incision made during the surgical procedure, one or more additional doses of the glutamine source is administered at fixed intervals of time after the first dose, and one or more doses is administered just prior to closure of all surgical incisions.