Patents by Inventor M. A. Stephens

M. A. Stephens has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).

  • Expression of heterologous DNA using the bacillus coagulans amylase gene
    Patent number: 5171673
    Abstract: A vector for high-level expression of a heterologous gene in Bacillus, including a DNA sequence which encodes all or part of the structural gene and contains the promoter and ribosome binding site of a Bacillus coagulans amylase gene; within or immediately downstream of the structural gene is a site for insertion of heterologous DNA; when inserted, the heterologous DNA is in the same translational reading frame as the amylase gene; the heterologous DNA thus is under control of the B. coagulans amylase gene regulatory elements and expressed at high levels. A signal-encoding sequence may be inserted independent of, or along with, the heterologous DNA leading to authentic secretion of the heterologous protein.
    Type: Grant
    Filed: July 18, 1988
    Date of Patent: December 15, 1992
    Assignee: Biotechnica International, Inc.
    Inventors: Alan Sloma, Nancy M. Hannett, M. A. Stephens, Cathy F. Rudolph, Gerald A. Rufo, Jr., Janice Pero