Patents by Inventor M. Rohan Fernando
M. Rohan Fernando has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240060113Abstract: A method for preserving and processing fetal nucleic acids located within maternal plasma is disclosed, wherein a sample of maternal blood containing fetal nucleic acids is treated to reduce both cell lysis of the maternal blood cells and deoxyribonuclease (DNase) and ribonuclease (RNase) activity within the fetal nucleic acids. The treatment of the sample aids in increasing the amount of fetal nucleic acids that can be identified and tested while maintaining the structure and integrity of the fetal nucleic acids.Type: ApplicationFiled: April 25, 2023Publication date: February 22, 2024Inventors: M Rohan Fernando, Kate Chao-Wei Chen
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Publication number: 20230374568Abstract: A method for preserving and processing cell-free nucleic acids located within a blood sample is disclosed, wherein a blood sample containing cell-free nucleic acids is treated to reduce both blood cell lysis and nuclease activity within the blood sample. The treatment of the sample aids in increasing the amount of cell-free nucleic acids that can be identified and tested while maintaining the structure and integrity of the nucleic acids.Type: ApplicationFiled: July 31, 2023Publication date: November 23, 2023Inventor: M. Rohan Fernando
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Patent number: 11761025Abstract: A method for preserving and processing cell-free nucleic acids located within a blood sample is disclosed, wherein a blood sample containing cell-free nucleic acids is treated to reduce both blood cell lysis and nuclease activity within the blood sample. The treatment of the sample aids in increasing the amount of cell-free nucleic acids that can be identified and tested while maintaining the structure and integrity of the nucleic acids.Type: GrantFiled: June 22, 2020Date of Patent: September 19, 2023Assignee: STRECK LLCInventor: M. Rohan Fernando
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Patent number: 11753682Abstract: A method of making a noninvasive molecular control and analysis is described. The method of making the noninvasive molecular control includes, predetermining a positive control for a condition of interest, selecting a cell line with a control marker for the condition of interest, amplifying release of cfDNA from the selected cell line, isolating the released cfDNA from the selected cell line, quantifying the control marker from the isolated cfDNA, determining a volume of the control marker for addition to an isolated control plasma, isolating the control plasma from a control sample, treating the isolated control plasma with a control stabilizer, determining a volume of the control plasma to add to the volume of control marker, combining the volume of the control marker with the isolated control plasma, and analyzing a sample against the noninvasive molecular control to determine the presence or absence of a condition of interest.Type: GrantFiled: March 6, 2017Date of Patent: September 12, 2023Assignee: Father Flanagan's Boys'HomeInventor: M. Rohan Fernando
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Publication number: 20230279493Abstract: The present disclosure provides kits, methods, and assays for detecting one or more mutations associated with hereditary or syndromic hearing loss. The method can comprise performing a multigene panel sequencing assay on a biological sample extracted from a subject to identify one or more mutations associated with hearing loss. The disclosed kits, methods, and assays may be particularly useful for determining whether an infant patient is a carrier for or is at risk for developing a hereditary hearing loss-related disorder such as Usher syndrome, Pendred syndrome, Jervell syndrome, and Lange-Nielsen syndrome.Type: ApplicationFiled: February 28, 2023Publication date: September 7, 2023Inventors: M. Rohan Fernando, Jianbing Qin
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Publication number: 20230180742Abstract: Compositions and Methods for Stabilizing Circulating Tumor Cells Methods and compositions for stabilizing a biological sample for analysis, comprising the steps of obtaining in a sample collection device a biological sample from a subject, especially blood, the biological sample including at least one circulating tumor cell from the subject. The methods may include a step of contacting the biological sample with a protective agent composition that includes a preservative agent, an optional anticoagulant, and a quenching agent to form a mixture that includes the protective agent composition and the sample.Type: ApplicationFiled: January 9, 2023Publication date: June 15, 2023Inventors: M. Rohan Fernando, Wayne L. Ryan, Brad Hunsley
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Patent number: 11634747Abstract: A method for preserving and processing fetal nucleic acids located within maternal plasma is disclosed, wherein a sample of maternal blood containing fetal nucleic acids is treated to reduce both cell lysis of the maternal blood cells and deoxyribonuclease (DNase) and ribonuclease (RNase) activity within the fetal nucleic acids. The treatment of the sample aids in increasing the amount of fetal nucleic acids that can be identified and tested while maintaining the structure and integrity of the fetal nucleic acids.Type: GrantFiled: January 19, 2010Date of Patent: April 25, 2023Assignee: STRECK LLCInventors: M. Rohan Fernando, Kate Chao-Wei Chen
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Patent number: 11547111Abstract: Compositions and Methods for Stabilizing Circulating Tumor Cells Methods and compositions for stabilizing a biological sample for analysis, comprising the steps of obtaining in a sample collection device a biological sample from a subject, especially blood, the biological sample including at least one circulating tumor cell from the subject. The methods may include a step of contacting the biological sample with a protective agent composition that includes a preservative agent, an optional anticoagulant, and a quenching agent to form a mixture that includes the protective agent composition and the sample.Type: GrantFiled: June 8, 2020Date of Patent: January 10, 2023Assignee: Streck, Inc.Inventors: M. Rohan Fernando, Wayne L. Ryan, Brad Hunsley
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Publication number: 20200318161Abstract: A method for preserving and processing cell-free nucleic acids located within a blood sample is disclosed, wherein a blood sample containing cell-free nucleic acids is treated to reduce both blood cell lysis and nuclease activity within the blood sample. The treatment of the sample aids in increasing the amount of cell-free nucleic acids that can be identified and tested while maintaining the structure and integrity of the nucleic acids.Type: ApplicationFiled: June 22, 2020Publication date: October 8, 2020Inventor: M. Rohan Fernando
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Publication number: 20200315163Abstract: Compositions and Methods for Stabilizing Circulating Tumor Cells Methods and compositions for stabilizing a biological sample for analysis, comprising the steps of obtaining in a sample collection device a biological sample from a subject, especially blood, the biological sample including at least one circulating tumor cell from the subject. The methods may include a step of contacting the biological sample with a protective agent composition that includes a preservative agent, an optional anticoagulant, and a quenching agent to form a mixture that includes the protective agent composition and the sample.Type: ApplicationFiled: June 8, 2020Publication date: October 8, 2020Inventors: M. Rohan Fernando, Wayne L. Ryan, Brad Hunsley
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Publication number: 20200283846Abstract: A method of making a noninvasive molecular control and analysis is described. The method of making the noninvasive molecular control includes, predetermining a positive control for a condition of interest, selecting a cell line with a control marker for the condition of interest, amplifying release of cfDNA from the selected cell line, isolating the released cfDNA from the selected cell line, quantifying the control marker from the isolated cfDNA, determining a volume of the control marker for addition to an isolated control plasma, isolating the control plasma from a control sample, treating the isolated control plasma with a control stabilizer, determining a volume of the control plasma to add to the volume of control marker, combining the volume of the control marker with the isolated control plasma, and analyzing a sample against the noninvasive molecular control to determine the presence or absence of a condition of interest.Type: ApplicationFiled: March 6, 2017Publication date: September 10, 2020Applicant: CFGEnome, LLCInventor: M. Rohan Fernando
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Publication number: 20200255819Abstract: Methods and devices for stabilizing a biological sample for analysis, comprising the steps of obtaining in a sample collection device a biological sample from a subject, the biological sample including at least one circulating cell-free first nucleic acid from the subject. The methods may include a step of contacting the biological sample while within the sample collection device with a protective agent composition that includes a preservative agent, an optional anticoagulant, and a quenching agent to form a mixture that includes the protective agent composition and the sample.Type: ApplicationFiled: April 28, 2020Publication date: August 13, 2020Inventors: Wayne L. Ryan, M. Rohan Fernando, Kausik Das
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Patent number: 10689686Abstract: A method for preserving and processing cell-free nucleic acids located within a blood sample is disclosed, wherein a blood sample containing cell-free nucleic acids is treated to reduce both blood cell lysis and nuclease activity within the blood sample. The treatment of the sample aids in increasing the amount of cell-free nucleic acids that can be identified and tested while maintaining the structure and integrity of the nucleic acids.Type: GrantFiled: April 8, 2019Date of Patent: June 23, 2020Assignee: Streck, Inc.Inventor: M. Rohan Fernando
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Publication number: 20190254273Abstract: Stabilizing compositions for stabilizing a post-phlebotomy, but pre-analysis, blood samples include a metabolic inhibitor, a protease inhibitor, a buffer system, an anticoagulant, and a solvent. The stabilizing compositions stabilize a post-phlebotomy blood sample to preserve the physiological state of the blood sample for later analysis. The analysis performed on the stabilized blood sample may determine the state of an analyte in a blood sample for diagnosis. The stabilizing compositions may stabilize the post-phlebotomy blood sample for at least 6 hours, and up to 672 hours. More preferably, the stabilizing composition has an undetectable level of formaldehyde before contact with the post-phlebotomy blood sample, so that cross linking of proteins and cross linking of proteins to nucleic acids in the post-phlebotomy blood sample is minimized. The stabilizing composition has a test sensitivity rate of 1 for up to 672 hours.Type: ApplicationFiled: June 7, 2017Publication date: August 22, 2019Applicant: CFGEnome, LLCInventor: M. Rohan Fernando
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Publication number: 20190233879Abstract: A method for preserving and processing cell-free nucleic acids located within a blood sample is disclosed, wherein a blood sample containing cell-free nucleic acids is treated to reduce both blood cell lysis and nuclease activity within the blood sample. The treatment of the sample aids in increasing the amount of cell-free nucleic acids that can be identified and tested while maintaining the structure and integrity of the nucleic acids.Type: ApplicationFiled: April 8, 2019Publication date: August 1, 2019Inventor: M. Rohan Fernando
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Patent number: 10294513Abstract: A method for preserving and processing cell-free nucleic acids located within a blood sample is disclosed, wherein a blood sample containing cell-free nucleic acids is treated to reduce both blood cell lysis and nuclease activity within the blood sample. The treatment of the sample aids in increasing the amount of cell-free nucleic acids that can be identified and tested while maintaining the structure and integrity of the nucleic acids.Type: GrantFiled: March 27, 2018Date of Patent: May 21, 2019Assignee: Streck, Inc.Inventor: M. Rohan Fernando
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Patent number: 10253351Abstract: A method for preserving and processing cell-free nucleic acids located within a blood sample is disclosed, wherein a blood sample containing cell-free nucleic acids is treated to reduce both blood cell lysis and nuclease activity within the blood sample. The treatment of the sample aids in increasing the amount of cell-free nucleic acids that can be identified and tested while maintaining the structure and integrity of the nucleic acids.Type: GrantFiled: March 27, 2018Date of Patent: April 9, 2019Assignee: Streck, Inc.Inventor: M. Rohan Fernando
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Patent number: 10144955Abstract: Methods for preserving and processing cell-free nucleic acids are disclosed. The method includes a blood collection tube including a composition formulated for stabilizing cell-free nucleic acids within a blood sample. The composition includes least one formaldehyde releaser preservative agent; ethylenediaminetetraacetic acid (EDTA); and one or more solvents; and at least some formaldehyde and up to about 1% formaldehyde as a result of the at least one formaldehyde releaser preservative agent. The collected blood sample is storable for at least, or about 7 days without cell lysis and without cell-free nucleic acid degradation of the blood sample due to DNase and RNase activity after blood draw. The method may include isolating the cell free nucleic acids from the stabilized blood sample and testing the isolated cell free nucleic acids from the stabilized blood sample to identify the presence, absence or severity of a disease state.Type: GrantFiled: February 12, 2018Date of Patent: December 4, 2018Assignee: Streck, Inc.Inventor: M. Rohan Fernando
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Publication number: 20180216165Abstract: A method for preserving and processing cell-free nucleic acids located within a blood sample is disclosed, wherein a blood sample containing cell-free nucleic acids is treated to reduce both blood cell lysis and nuclease activity within the blood sample. The treatment of the sample aids in increasing the amount of cell-free nucleic acids that can be identified and tested while maintaining the structure and integrity of the nucleic acids.Type: ApplicationFiled: March 27, 2018Publication date: August 2, 2018Inventor: M. Rohan Fernando
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Publication number: 20180163256Abstract: A method for preserving and processing cell-free nucleic acids located within a blood sample is disclosed, wherein a blood sample containing cell-free nucleic acids is treated to reduce both blood cell lysis and nuclease activity within the blood sample. The treatment of the sample aids in increasing the amount of cell-free nucleic acids that can be identified and tested while maintaining the structure and integrity of the nucleic acids.Type: ApplicationFiled: February 12, 2018Publication date: June 14, 2018Inventor: M. Rohan Fernando