Abstract: Compositions and regimens useful in treating type I citrullenemia are provided. The compositions include recombinant adeno-associated virus (rAAV) with a transthyretin enhancer and promoter driving expression of a human Argininosuccinate Synthase 1 (ASS1).

Abstract: A system and method for monitoring at least one storage provision in an aircraft has at least one sensor for sensing at least one object positioned in an interior space of the at least one storage provision, at least one indicator associated with the at least one storage provision, and a controller communicatively coupled to the at least one sensor, the at least one indicator, and at least one phase of flight controller. The controller has processing circuitry configured to activate the at least one sensor, receive object information from the at least one sensor, receive phase of flight information from the phase of flight controller, and when the object information pertains to at least one sensed object in the at least one storage provision, and the phase of flight information pertains to landing or deboarding of the aircraft, activate the at least one indicator.

Abstract: Provided is a recombinant adeno-associated virus (rAAV) having an AAVhu68 capsid and a vector genome which comprises a nucleic acid sequence encoding a functional human arylsulfatase A (ARSA). Also provided are a production system useful for producing the rAAV, a pharmaceutical composition comprising the rAAV, and a method of treating a subject having metachromatic leukodystrophy, or ameliorating symptoms of metachromatic leukodystrophy, or delaying progression of metachromatic leukodystrophy via administering an effective amount of rAAV to a subject in need thereof.

Abstract: An insulation system for beverage containers comprising: an inner container surrounding a plurality of cylindrical beverage containers contained in an inner container; a handle included in an inner container top panel; an outer container having a cavity defined in the outer container for receiving the inner container; a pair of lateral spacers disposed lengthwise along corresponding inner upper corners of the out container; an airspace defined between an inner container side panel and an external container side panel having a polygon cross-section; and, a handle access opening centrally defined in an outer container top panel allowing access to the handle of the inner container so that the beverage containers, inner container and out container can be transported using the handle wherein the width of the handle access opening is about one third the length of the outer panel top opening.

Abstract: The present disclosure relates to the seminal discovery of a generation and use of genetically engineered Vibrio sp. Provided is the use of the genetically engineered bacteria for the construction, maintenance, manipulation, and/or propagation of DNA constructs; protein expression; protein secretion; vectors and other metabolic tools; metabolic engineering; expression of cellular extracts for cell-free biology; shuttle vectors; cloning vectors; and for synthetic biology applications. The disclosure also relates to the use of the replication machinery of Vibrio sp. as a cloning or expression vector for replication of recombinant DNA constructs. The disclosure also relates to methods of use of the above.

Abstract: Compositions and methods for treating short bowel syndrome in a subject are provided. A viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding a GLP-2 receptor agonist fusion protein and regulatory sequences which direct expression thereof.

Abstract: Compositions and methods for treating hypoparathyroidism in a subject are provided. A viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding a PTH fusion protein and regulatory sequences which direct expression thereof.

Abstract: Compositions and methods for killing or inhibiting the growth of pathogenic fungi. A composition includes water, a solubilizer, and an essential oil extracted from one or more of lemongrass, cassia, eucalyptus, ajowan, clove leaf, or oregano.

Abstract: Provided is a recombinant adeno-associated virus (rAAV) having an AAVhu68 capsid and a vector genome which comprises a nucleic acid sequence encoding a functional human arylsulfatase A (ARSA). Also provided are a production system useful for producing the rAAV, a pharmaceutical composition comprising the rAAV, and a method of treating a subject having metachromatic leukodystrophy, or ameliorating symptoms of metachromatic leukodystrophy, or delaying progression of metachromatic leukodystrophy via administrating an effective amount of the rAAV to a subject in need thereof.

Abstract: A method of correcting singletons in a selected AAV sequence in order to increasing the packaging yield, transduction efficiency, and/or gene transfer efficiency of the selected AAV is provided. This method involves altering one or more singletons in the parental AAV capsid to conform the singleton to the amino acid in the corresponding position(s) of the aligned functional AAV capsid sequences.

Abstract: Compositions and methods for treating metabolic diseases in a subject are provided. A viral vector is provided which includes a nucleic acid molecule comprising a sequence encoding a GLP-1 receptor agonist fusion protein and regulatory sequences which direct expression thereof.

Abstract: A method of correcting singletons in a selected AAV sequence in order to increasing the packaging yield, transduction efficiency, and/or gene transfer efficiency of the selected AAV is provided. This method involves altering one or more singletons in the parental AAV capsid to conform the singleton to the amino acid in the corresponding position(s) of the aligned functional AAV capsid sequences.

Abstract: A dual vector system for treating ornithine transcarbamylase deficiency is provided. The system includes ((a) a gene editing AAV comprising a first AAV rh79 capsid and a first vector genome comprising a 5? ITR, a sequence encoding a meganuclease that targets PCSK9 under control of regulatory sequences that direct expression of the meganuclease in a target cell comprising a PCSK9 gene, and a 3? ITR; and (b) a donor AAV vector comprising a second AAV capsid and a second AAV rh79 vector genome comprising: a 5?ITR, a 5? homology directed recombination (HDR) arm, a transgene encoding ornithine transcarbamylase (OTC) and regulatory sequences that direct expression of the transgene in the target cell, a 3? HDR arm, and a 3? ITR.

Abstract: Catheter having an elongate tubular shaft including an inflation lumen and a guidewire lumen defined therein, the guidewire lumen extending along at least a distal length of an inner tubular member of the elongate tubular shaft. The catheter includes a distal tip member having a proximal end and a distal end, wherein the distal tip member is monolithic and the proximal end of the distal tip member is secured to a distal end of the inner tubular member. The catheter includes a balloon having a proximal portion and a distal portion, the proximal portion of the balloon sealingly coupled to the distal portion of the elongate tubular shaft. The distal portion of the balloon is sealingly coupled to the distal tip member and the balloon defines an inner chamber in communication with the inflation lumen, wherein the proximal end of the distal tip member is disposed within the inner chamber.

Abstract: An apparatus including a body, a shaft assembly extending distally from the body, and an end effector configured to grasp and transmit RF energy to the tissue. The end effector includes a first jaw having a first tissue grasping feature and a second jaw. The second jaw is pivotably coupled to the first jaw between an open position, a partially closed position, and a closed position. The second jaw includes a proximal taper having a proximal electrode surface, a distal taper including a distal electrode surface, and a juncture between the proximal and distal electrode surface. The juncture is spaced further from the first tissue grasping feature compared to the proximal and distal end while the second jaw is in the partially closed position. The proximal and distal electrode surface deform to define a gap with the first tissue grasping feature while in the closed position.

Abstract: Systems and methods receive an input that triggers a request for a random numeric output associated with an outcome that indicates whether resource(s) are distributed to a player account of a player of a gaming application, and transmit the request for generation of the random numeric output to a third party server, the request indicating parameter(s) configured to influence the random numeric output and including data indicating weighted variables used to influence the outcome. A digitally signed electronic output file including the random numeric output is received, the digitally signed electronic output file being encrypted and including a stamp of authentication thereby cryptographically binding an electronic identity to the random numeric output. Key(s) stored to a key index are accessed and the digitally signed electronic output file is verified using the key(s). The random numeric output is authenticated and applied to a promotional draw to determine the outcome.

Abstract: Systems receive a request from a player device for a random numeric output associated with an outcome that indicates whether resource(s) are to be distributed to a player account of a player of a computer game. The request is authenticated, which includes determining a geographic location of the player from an identifier of the player device, and a current amount of player points already attributed to the player account are determined from player data associated with the player account. An API request associated with the random numeric output to be generated by the outside server is generated and transmitted to an outside server that includes the current amount of player points. A randomly generated numeric input is received from the outside server and based on the current amount of player points, and the outcome is determined based on the randomly generated numeric input and the resource(s) are distributed.

Abstract: Systems and methods train, using training data, a prediction model by iteratively predicting a target variable value of a churn-based event associated with an online video game application by identifying an error between a prediction and the target variable value and modifying weights of the prediction model for multiple iterations, the training data includes information obtained from the online video game application and a partner computer application. The partner computer application stores resource data of real-world resources that are stored to a real-world location, and the information includes specific data from a duration of gameplay via the online video game application, a quantity of instances of gameplay, and a quantity of resource transactions of the virtual resource. The prediction model is deployed and applied to user data of users to predict the churn-based event, where user data is restricted by history date for a predefined number of days.

Abstract: Systems and methods extract raw user data of users of an online video game application (OVGA) and append raw user data to a dataset. Data object(s) of the dataset are segmented into, and attribute(s) are derived from field(s) of the dataset, and the attribute(s) include a time between initiation of user engagement with the OVGA and user disengagement with the OVGA by the users. Missing data is imputed with substitute values, and entity extraction is performed using natural language processing to extract specific data that is then classified and stored to a relational database. The relational database is versioned to generate versioned data, and a target variable value is inserted into an iterative training and testing loop to train a prediction model using the versioned data, where the training is based on adjusting weights and iterative calculations to predict the a date of a churn-based event.