Abstract: A method of determination of a trajectory of a living tissue, in which subsequent frames representing images of living tissue are acquired with at least a first imaging device and at least a first segment of a trajectory of the living tissue is determined with a use of relative displacements of at least first subset of subsequent frames and recalculation thereof to coordinates corresponding to time of frame acquisition to form a vector Tm elements tm wherein element tm is at least one coordinate of living tissue, element tm of vector Tm is determined with a use of relative displacements pm,k of at least two preceding frames.