Patents by Inventor Maciej Szczepanik
Maciej Szczepanik has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20230175036Abstract: Provided is a compound comprising the structure: (SIG)-(SI-MOD)m. In this compound, SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. With this compound, when MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided is a method of determining whether a sample comprises an activator, using the above-described compound. Additionally provided is a method of determining whether a cell comprises a nitroreductase using the above-described compound where nitroreductase is the activator. Further provided is a method of determining whether a mammalian cell is hypoxic using the above-described compound where nitroreductase is the activator.Type: ApplicationFiled: January 30, 2023Publication date: June 8, 2023Applicant: Enzo Life Sciences, Inc.Inventors: Irina LEBEDEVA, Wayne Forrest PATTON, Maciej SZCZEPANIK, Yuejun XIANG, Praveen PANDE
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Patent number: 11661621Abstract: Provided is a compound having the structure: (SIG)-(SI-MOD)m where SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample, such as a cell, comprises an activator, such as a nitroreducase, using the compound. Further provided are methods of determining whether a mammalian cell is hypoxic using the compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase using the compound where nitroreductase is the activator is also provided.Type: GrantFiled: May 28, 2020Date of Patent: May 30, 2023Assignee: ENZO LIFE SCIENCES, INC.Inventors: Irina Lebedeva, Wayne Forrest Patton, Maciej Szczepanik, Yuejun Xiang, Praveen Pande
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Patent number: 11162939Abstract: Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. In this assay, the amount of the signal or the first member of the second binding pair bound to the binding agent is inversely proportional to the analyte in the sample.Type: GrantFiled: March 9, 2020Date of Patent: November 2, 2021Assignee: Enzo Life Sciences, Inc.Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
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Publication number: 20210325375Abstract: Provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.Type: ApplicationFiled: June 17, 2021Publication date: October 21, 2021Applicant: Enzo Life Science, Inc.Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
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Patent number: 11073512Abstract: Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. Additionally provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.Type: GrantFiled: November 28, 2018Date of Patent: July 27, 2021Assignee: Enzo Life Sciences, Inc.Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
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Publication number: 20210164046Abstract: A composition comprising micron or submicron particles covered by a monolayer of nucleic acid wherein the nucleic acid may be recovered from the submicron particles is claimed. Methods of attaching a nucleic acid to an object for authentication and methods of authenticating an object are also claimed.Type: ApplicationFiled: February 15, 2021Publication date: June 3, 2021Inventors: Michael E. HOGAN, Lawrence JUNG, Yuhua SUN, Maciej Szczepanik
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Patent number: 10920274Abstract: A composition comprising submicron particles covered by a monolayer of nucleic acid wherein the nucleic acid may be recovered from the submicron particles is claimed. Methods of attaching a nucleic acid to an object for authentication and methods of authenticating an object are also claimed.Type: GrantFiled: February 7, 2018Date of Patent: February 16, 2021Assignee: APDN (B.V.I.) Inc.Inventors: Michael E. Hogan, Lawrence Jung, Nicole Richter, Yuhua Sun, Maciej Szczepanik
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Publication number: 20200291448Abstract: Provided is a compound having the structure: (SIG)-(SI-MOD)m where SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample, such as a cell, comprises an activator, such as a nitroreducase, using the compound. Further provided are methods of determining whether a mammalian cell is hypoxic using the compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase using the compound where nitroreductase is the activator is also provided.Type: ApplicationFiled: May 28, 2020Publication date: September 17, 2020Applicant: ENZO LIFE SCIENCES, INC.Inventors: Irina LEBEDEVA, Wayne Forest PATTON, Maciej SZCZEPANIK, Yuejun XIANG, Praveen PANDE
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Publication number: 20200284786Abstract: Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. In this assay, the amount of the signal or the first member of the second binding pair bound to the binding agent is inversely proportional to the analyte in the sample.Type: ApplicationFiled: March 9, 2020Publication date: September 10, 2020Applicant: Enzo Life Science, Inc.Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
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Patent number: 10718005Abstract: Provided is a compound having the structure: (SIG)-(SI-MOD)m where SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample, such as a cell, comprises an activator, such as a nitroreducase, using the compound. Further provided are methods of determining whether a mammalian cell is hypoxic using the compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase using the compound where nitroreductase is the activator is also provided.Type: GrantFiled: November 1, 2018Date of Patent: July 21, 2020Assignee: Enzo Life Sciences, IncInventors: Irina Lebedeva, Wayne Forrest Patton, Maciej Szczepanik, Yuejun Xiang, Praveen Pande
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Patent number: 10627397Abstract: Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. In this assay, the amount of the signal or the first member of the second binding pair bound to the binding agent is inversely proportional to the analyte in the sample.Type: GrantFiled: April 9, 2019Date of Patent: April 21, 2020Assignee: Enzo Life Sciences, Inc.Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
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Publication number: 20190285621Abstract: Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. In this assay, the amount of the signal or the first member of the second binding pair bound to the binding agent is inversely proportional to the analyte in the sample.Type: ApplicationFiled: April 9, 2019Publication date: September 19, 2019Applicant: Enzo Life Science, Inc.Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
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Patent number: 10302633Abstract: Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. In this assay, the amount of the signal or the first member of the second binding pair bound to the binding agent is inversely proportional to the analyte in the sample.Type: GrantFiled: July 23, 2018Date of Patent: May 28, 2019Assignee: Enzo Life Sciences, Inc.Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
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Publication number: 20190145964Abstract: Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. Additionally provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.Type: ApplicationFiled: November 28, 2018Publication date: May 16, 2019Applicant: Enzo Life Science, Inc. c/o Enzo Biochem, Inc.Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
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Publication number: 20190119719Abstract: Provided is a compound having the structure: (SIG)-(SI-MOD)m where SIG is a signaling molecule, SI is a self-immolative structure bound to SIG such that SIG has a reduced signal relative to the signal of SIG without SI, MOD is a moiety bound to SI that is subject to modification by an activator, and m is an integer from 1 to about 10. When MOD is modified by an activator, SI is destabilized and self-cleaved from SIG such that SIG generates an increased signal. Also provided are methods of determining whether a sample, such as a cell, comprises an activator, such as a nitroreducase, using the compound. Further provided are methods of determining whether a mammalian cell is hypoxic using the compound where nitroreductase is the activator. A method of detecting a microorganism that comprises a nitroreductase using the compound where nitroreductase is the activator is also provided.Type: ApplicationFiled: November 1, 2018Publication date: April 25, 2019Applicant: ENZO LIFE SCIENCES, INC.Inventors: Irina Lebedeva, Wayne Forrest Patton, Maciej Szczepanik, Yuejun Xiang, Praveen Pande
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Patent number: 10184934Abstract: Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. Additionally provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.Type: GrantFiled: May 31, 2017Date of Patent: January 22, 2019Assignee: Enzo Life Sciences, Inc.Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
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Publication number: 20180335426Abstract: Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. In this assay, the amount of the signal or the first member of the second binding pair bound to the binding agent is inversely proportional to the analyte in the sample.Type: ApplicationFiled: July 23, 2018Publication date: November 22, 2018Applicant: Enzo Life Science, Inc.Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
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Patent number: 10060910Abstract: Provided is a composition comprising an analyte bound to a polymer where the analyte is less than about 2000 MW and the polymer further comprises more than one signal or first member of a second binding pair. Also provided is an assay for an analyte comprising combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. Additionally provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.Type: GrantFiled: July 24, 2012Date of Patent: August 28, 2018Assignee: Enzo Life Sciences, Inc.Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin
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Publication number: 20180237854Abstract: A composition comprising submicron particles covered by a monolayer of nucleic acid wherein the nucleic acid may be recovered from the submicron particles is claimed. Methods of attaching a nucleic acid to an object for authentication and methods of authenticating an object are also claimed.Type: ApplicationFiled: February 7, 2018Publication date: August 23, 2018Inventors: Michael E. Hogan, Lawrence Jung, Nicole Richter, Yuhua Sun, Maciej Szczepanik
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Publication number: 20170315113Abstract: Provided is a composition comprising an analyte bound covalently or through a first binding pair to a polymer. In this composition, the analyte is less than about 2000 MW; the polymer further comprises more than one signal or first member of a second binding pair; and the analyte is not a member of the first binding pair or the second binding pair. Also provided is an assay for an analyte. The assay comprises: combining a sample suspected of containing the analyte with the above-described composition and a binding agent that binds to the analyte; and detecting the signal or the first member of the second binding pair that is bound to the binding agent. Additionally provided is a multisignal labeling reagent comprising a first polymer covalently bound to (a) a reactive group or a first member of a first binding pair, and (b) more than one digoxigenin molecule.Type: ApplicationFiled: May 31, 2017Publication date: November 2, 2017Applicant: Enzo Life Science, Inc., c/o Enzo Biochem, Inc.Inventors: Jack Coleman, Maciej Szczepanik, Richard Jin