Abstract: The present disclosure relates to methods, compositions, and kits for isolating target nucleic acids below a target size from a sample comprising nucleic acid components. In some embodiments, the methods involve one or more aqueous two-phase system (ATPS) compositions, at least one solid phase medium, and at least one buffer. Some embodiments provide a kit comprising one or more ATPS compositions, at least one solid phase medium, and at least one buffer. Other embodiments provide methods for diagnosing a disease or condition using the methods described herein.

Abstract: The present disclosure relates to methods, compositions, and kits for concentrating and purifying at least one target analyte from a clinical biological sample. In some embodiments, the methods involve one or more aqueous two-phase system (ATPS) compositions and at least one solid phase medium. Some embodiments provide a kit comprising one or more ATPS compositions, a binding buffer; and a solid phase medium. Other embodiments provide methods of treating cancers or infectious diseases in a patient in need thereof.

Abstract: Provided is a method for isolating and concentrating nucleic acids of selected target sizes (e.g., in increments less than 1000 base pairs) from a biological fluid mixture comprising combining the biological fluid mixture and a first aqueous two-phase system (ATPS) formed from a first phase forming polymer or surfactant component dissolved in a first phase solution, and a second phase solution, such that target nucleic acid fragments below a desired target size partition to said second phase solution and contaminants partition to the first phase solution, extracting and mixing the second phase solution with a second ATPS formed from a second phase forming polymer or surfactant component dissolved in a third phase solution and a fourth phase solution, such that the target nucleic acid fragments partition to and concentrate in the third phase solution, and recovering the concentrated target nucleic acid fragments from the third phase solution.

Abstract: A method for measuring fatigue on a metal or metal alloy structural part, which includes mounting a fatigue gage on a surface of the part, the fatigue gage being the same material as the part, applying electrical power on the gage and measuring the resistance of the fatigue gage, inspecting the fatigue gage at various time intervals by applying electric power on the gage and measuring the resistance of the fatigue gage; and analyzing the change of resistance of the fatigue gage at the various time intervals to determine fatigue on the part.

Abstract: A method for measuring fatigue on a metal or metal alloy structural part, which includes mounting a fatigue gage on a surface of the part, the fatigue gage being the same material as the part, applying electrical power on the gage and measuring the resistance of the fatigue gage, inspecting the fatigue gage at various time intervals by applying electric power on the gage and measuring the resistance of the fatigue gage; and analyzing the change of resistance of the fatigue gage at the various time intervals to determine fatigue on the part.