Abstract: A system is provided for measuring and assessing hemodynamics in an anatomical structure of a subject, along with a method for image processing hemodynamics in at least a part of an anatomical structure in video images acquired from a subject. The system and method relates to measuring and assessing hemodynamics in, around and near the surface, in particular the gastrointestinal wall, of the gastrointestinal tract of a subject. A method for image processing hemodynamics in at least a part of an anatomical structure in video images acquired from a subject may performing image analysis of at least one video sequence acquired after a fluorescent contrast agent has been supplied to the subject, calculating intensity values in one or more regions of interest based on the image analysis, and determining the perfusion slope of the flow of the fluorescent contrast agent through at least one of said regions of interest.