Abstract: A display substrate includes a lower base substrate, an organic insulation layer, an insulation protrusion, a pixel electrode and a lower alignment layer. The lower base substrate comprises a plurality of unit pixel areas, the plurality of unit pixel areas being divided into a plurality of sub-pixel areas arranged in a matrix pattern. An organic insulation layer is formed on the lower base substrate. An insulation protrusion is formed on the organic insulation layer corresponding to a boundary of sub-pixel areas. A pixel electrode is formed on the organic insulation layer and the insulation protrusion. In addition, the lower alignment layer is formed on the pixel electrode and irradiated with ultraviolet (UV) light to provide different alignment directions that correspond to the sub-pixel areas.