Patents by Inventor Magali Remaud-Simeon
Magali Remaud-Simeon has filed for patents to protect the following inventions. This listing includes patent applications that are pending as well as patents that have already been granted by the United States Patent and Trademark Office (USPTO).
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Publication number: 20240002423Abstract: The present invention provides protected tetrasaccharides, their process of preparation and their use in the synthesis of oligosaccharides, in particular fragments of O-antigens from Shigella flexneri, for example of serotype 1a, 1b, 2a, 2b, 3a, X, 4a, 4b, 5a, 5b, 7a or 7b.Type: ApplicationFiled: September 8, 2023Publication date: January 4, 2024Inventors: Laurence MULARD, Guillaume LE HEIGET, Zhaohu HU, Louis-Antoine BAREL, Isabelle ANDRE, Claire MOULIS, Magali REMAUD-SIMEON, Sophie BARBE, Mounir BENKOULOUCHE, Akli BEN IMEDDOURENE
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Patent number: 11834468Abstract: The present invention provides protected tetrasaccharides, their process of preparation and their use in the synthesis of oligosaccharides, in particular fragments of O-antigens from Shigella flexneri, for example of serotype 1a, 1b, 2a, 2b, 3a, X, 4a, 4b, 5a, 5b, 7a or 7b.Type: GrantFiled: July 3, 2018Date of Patent: December 5, 2023Assignees: INSTITUT PASTEUR, INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUEInventors: Laurence Mulard, Guillaume Le Heiget, Zhaohu Hu, Louis-Antoine Barel, Isabelle Andre, Claire Moulis, Magali Remaud-Simeon, Sophie Barbe, Mounir Benkoulouche, Akli Ben Imeddourene
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Publication number: 20230295679Abstract: The present invention relates to a process for preparing an alkyl polyglucoside by enzymatic catalysis, using sucrose or an analogue thereof as substrate and making it possible to obtain a large diversity of alkyl polyglucosides in terms of size and structure of the glucoside part thereof, making possible the obtaining of an alkyl polyglucoside with a number of glucosyl units that can be adjusted from 2 to 200 glucosyl units. The process also makes it possible to adjust the linear or branched structure of the carbohydrate part of the alkyl polyglucoside obtained, and also the nature of the glycosidic bonds linking the glucose residues within the carbohydrate part.Type: ApplicationFiled: July 2, 2021Publication date: September 21, 2023Applicants: INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE, Institut National de Recherche Pour l'Agriculture, l'Alimentation et l'Environnement, Centre National de la Recherche Scientifique (CNRS)Inventors: Etienne SEVERAC, Claire MOULIS, David GUIEYSSE, Sandrine MOREL, Magali REMAUD-SIMEON
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Patent number: 11253537Abstract: Polypeptides having the ability to specifically form connections of glucosyl units in alpha 1,3 on an acceptor having at least one hydroxyl moiety are presented. The polypeptides include i) the pattern I of sequence SEQ ID NO: 1, ii) the pattern II of sequence SEQ ID NO: 2, iii) the pattern III of sequence SEQ ID NO: 3, and iv) the pattern IV of sequence SEQ ID NO: 4, or derivates from one or several of said patterns, wherein the polypeptide furthermore has an aspartic residue (D) at position 5 of the pattern II (SEQ ID NO: 2), a glutamic acid residue (E) at position 6 of the pattern III (SEQ ID NO: 3) an an aspartic acid residue (D) at position 6 of the pattern IV (SEQ ID NO: 4). Methods for producing acceptors connected to glucosyl units in alpha 1,3 using the polypeptides are also provided.Type: GrantFiled: June 29, 2020Date of Patent: February 22, 2022Assignees: INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT, INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)Inventors: Magali Remaud-Simeon, Marlene Vuillemin, Claire Moulis, Pierre Monsan, Sandrine Morel
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Patent number: 10842808Abstract: Polypeptides having the ability to specifically form connections of glucosyl units in alpha 1,3 on an acceptor having at least one hydroxyl moiety are presented. The polypeptides include i) the pattern I of sequence SEQ ID NO: 1, ii) the pattern II of sequence SEQ ID NO: 2, iii) the pattern III of sequence SEQ ID NO: 3, and iv) the pattern IV of sequence SEQ ID NO: 4, or derivates from one or several of said patterns, wherein the polypeptide furthermore has an aspartic residue (D) at position 5 of the pattern II (SEQ ID NO: 2), a glutamic acid residue (E) at position 6 of the pattern III (SEQ ID NO: 3) and an aspartic acid residue (D) at position 6 of the pattern IV (SEQ ID NO: 4). Methods for producing acceptors connected to glucosyl units in alpha 1,3 using the polypeptides are also provided.Type: GrantFiled: September 22, 2017Date of Patent: November 24, 2020Assignees: INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT, INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE (CNRS)Inventors: Magali Remaud-Simeon, Marlene Vuillemin, Claire Moulis, Pierre Monsan, Sandrine Morel
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Publication number: 20200323899Abstract: Polypeptides having the ability to specifically form connections of glucosyl units in alpha 1,3 on an acceptor having at least one hydroxyl moiety are presented. The polypeptides include i) the pattern I of sequence SEQ ID NO: 1, ii) the pattern II of sequence SEQ ID NO: 2, iii) the pattern III of sequence SEQ ID NO: 3, and iv) the pattern IV of sequence SEQ ID NO: 4, or derivates from one or several of said patterns, wherein the polypeptide furthermore has an aspartic residue (D) at position 5 of the pattern II (SEQ ID NO: 2), a glutamic acid residue (E) at position 6 of the pattern III (SEQ ID NO: 3) an an aspartic acid residue (D) at position 6 of the pattern IV (SEQ ID NO: 4). Methods for producing acceptors connected to glucosyl units in alpha 1,3 using the polypeptides are also provided.Type: ApplicationFiled: June 29, 2020Publication date: October 15, 2020Inventors: Magali REMAUD-SIMEON, Marlene VUILLEMIN, Claire MOULIS, Pierre MONSAN, Sandrine MOREL
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Patent number: 10696995Abstract: The present invention relates to a method for producing a glycosylated synthon or a monomer. Said method includes at least one step of placing at least one glycan-saccharase in the presence of at least one hydroxylated synthon and at least one saccharose. The invention also relates to a method for producing a glyco(co)polymer, including polymerizing at least two monomers separately obtained from the enzymatic glycosylation method according to the invention, and to a method for producing a glyco(co)polymer, preferably a block glyco(co)polymer, including coupling at least two monomers separately obtained from the enzymatic glycosylation method according to the invention.Type: GrantFiled: March 17, 2016Date of Patent: June 30, 2020Assignees: INSTITUT NATIONAL DE RECHERCHE POUR L'AGRICULTURE, L'ALIMENTATION ET L'ENVIRONNEMENT, INSTITUT NATIONAL DES SCIENCES APPIQUEES DE TOULOUSE (INSAT), CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE DE BORDEAUX, INSTITUT POLYTECHNIQUE DE BORDEAUXInventors: Isabelle Andre, Stephane Grelier, David Guieysse, Alvaro Lafraya, Pierre Monsan, Claire Moulis, Frederic Peruch, Magali Remaud-Simeon, Marlene Vuillemin
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Publication number: 20200165285Abstract: The present invention provides protected tetrasaccharides, their process of preparation and their use in the synthesis of oligosaccharides, in particular fragments of O-antigens from Shigella flexneri, for example of serotype 1a, 1b, 2a, 2b, 3a, X, 4a, 4b, 5a, 5b, 7a or 7b.Type: ApplicationFiled: July 3, 2018Publication date: May 28, 2020Inventors: Laurence MULARD, Guillaume LE HEIGET, Zhaohu HU, Louis-Antoine BAREL, Isabelle ANDRE, Claire MOULIS, Magali REMAUD-SIMEON, Sophie BARBE, Mounir BENKOULOUCHE, Akli BEN IMEDDOURENE
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Patent number: 10647739Abstract: The invention relates to a method for producing derivatives of O-?-glucosylated flavonoid, comprising at least one step of incubating a glucansucrase with a flavonoid and at least one sucrose, the flavonoid being a flavonoid which is monohydroxylated or hydroxylated in a non-vicinal manner on the B cycle. The invention also relates to novel O-?-glucosylated flavonoid derivatives, and to the use thereof.Type: GrantFiled: March 24, 2015Date of Patent: May 12, 2020Assignees: INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, INSTITUTE NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUEInventors: Sandrine Morel, Isabelle Andre, Yoan Brison, Emmanuelle Cambon, Yannick Malbert, Denis Pompon, Magali Remaud-Simeon, Philippe Urban
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Patent number: 10358663Abstract: A method of producing 2,4-dihydroxybutyric acid (2,4-DHB) by a synthetic pathway that includes transforming malate into 4-phospho-malate using a malate kinase, then transforming 4-phospho-malate into malate-4-semialdehyde using a malate semialdehyde dehydrogenase, and then transforming malate-4-semialdehyde into 2,4-DHB using a DHB dehydrogenase.Type: GrantFiled: November 18, 2015Date of Patent: July 23, 2019Assignee: ADISSEO FRANCE S.A.S.Inventors: Thomas Walther, Helene Cordier, Christopher Topham, Isabelle Andre, Magali Remaud-Simeon, Robert Huet, Jean-Marie Francois
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Patent number: 10316300Abstract: The invention relates to: —a protein having dextran-saccharase activity and the sequence SEQ ID NO: 1 for an amino acid sequence; —a protein having dextran-saccharase activity and the sequence SEQ ID NO: 2 for an amino acid sequence; —a complex containing a substrate and a protein with dextran-saccharase activity —a method for synthesizing dextrans; and —dextrans. The invention also relates to a method for synthesizing gluco-oligosaccharides and gluco-conjugates and to the resulting products.Type: GrantFiled: July 21, 2015Date of Patent: June 11, 2019Assignees: INSTITUT NATIONAL DES SCIENCES APPLIQUEES TOULOUSE, INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE PAUL SABATIER TOULOUSE IIIInventors: Marlène Vuillemin, Marion Claverie, Claire Moulis, Magali Remaud-Simeon, Pierre Monsan, Etienne Severac, Catherine Fontagne-Faucher
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Patent number: 10308724Abstract: The subject matter of the invention is dextrans which have between 95% and 99% of ?-1,6 glucosidic bonds, a weight-average molar mass Mw at least equal to 0.7×109 g.mol?1, and a dispersity index D of between 1.3 and 3. The invention also relates to a dextran saccharase which makes it possible to produce such dextrans, and to a method for producing said dextrans.Type: GrantFiled: June 19, 2015Date of Patent: June 4, 2019Assignees: INSTITUT NATIONAL DES SCIENCES APPLIQUEES TOULOUSE, INSTITUT NATIONAL DE LA RECHERCHE AGRONOMIQUE, CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, UNIVERSITE DE BORDEAUX, INSTITUT POLYTECHNIQUE DE BORDEAUXInventors: Marlène Vuillemin, Marion Claverie, Claire Moulis, Magali Remaud-Simeon, Florent Grimaud, Pierre Monsan, Agnès Sabate, Catherine Garnier, Marguerite Dols-Lafargue, Patrick Lucas
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Patent number: 10167346Abstract: Compositions for improving the health of a subject comprise alpha-(1,2)-branched alpha-(1,6) oligodextrans, preferably with an average molecular weight between about 10 kDa and 70 kDa, between about 10% and 50% alpha-(1,2)-osidic side chains, and having at least partial indigestibility in the subject. Methods for improving the health of a subject comprise administering the composition to a subject in an amount effective to improve gut health, or to prevent or treat a gastrointestinal disorder, a cholesterol-related disorder, diabetes, or obesity. Methods for making oligodextrans having controlled size and controlled degree of branching comprise providing alpha-(1,6) oligodextrans having an average molecular weight between 0.5 and 100 kDa and introducing at least 10% alpha-(1,2)-osidic side chains onto the alpha-(1,6) oligodextrans.Type: GrantFiled: October 21, 2016Date of Patent: January 1, 2019Assignee: Tate & Lyle Ingredients France SASInventors: Thierry Naeye, Alexandra Einerhand, Michel Lopez, Susan M. Potter, Magali Remaud-Siméon, Pierre Frédéric Emmanuel Monsan
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Publication number: 20180258456Abstract: The present invention relates to a method for producing a glycosylated synthon or a monomer. Said method includes at least one step of placing at least one glycan-saccharase in the presence of at least one hydroxylated synthon and at least one saccharose. The invention also relates to a method for producing a glyco(co)polymer, including polymerizing at least two monomers separately obtained from the enzymatic glycosylation method according to the invention, and to a method for producing a glyco(co)polymer, preferably a block glyco(co)polymer, including coupling at least two monomers separately obtained from the enzymatic glycosylation method according to the invention.Type: ApplicationFiled: March 17, 2016Publication date: September 13, 2018Inventors: Isabelle Andre, Stephane Grelier, David Guieysse, Alvaro Lafraya, Pierre Monsan, Claire Moulis, Frederic Peruch, Magali Remaud-Simeon, Marlene Vuillemin
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Patent number: 10017748Abstract: The present invention relates to a recombinant process for the production of truncated or mutated dextransucrases while conserving the enzymatic activity or their specificity in the synthesis of the ?-1,6 bonds. The present invention relates to nucleic acid sequences of truncated or mutated dextransucrases, vectors containing the nucleic acid sequences and host cells transformed by sequences encoding truncated or mutated dextransucrases. In another aspect, the invention concerns a method for producing, in a recombinant manner, truncated or mutated dextransucrases which conserve their enzymatic activity or which conserve their specificity in the synthesis of ?-1,6 bonds and can produce, from saccharose, dextrans with high molar mass and modified rheological properties compared with the properties of dextran obtained with the native enzyme and isomalto-oligosaccharides with a controlled molar mass and dextrans.Type: GrantFiled: June 23, 2016Date of Patent: July 10, 2018Assignees: CENTRE NATIONAL DE LA RECHERCHE SCIENTIFIQUE, INSTITUT NATIONAL DE RECHERCHE AGRONOMIQUE, INSTITUT NATIONAL DES SCIENCES APPLIQUEES DE TOULOUSEInventors: Pierre F. Monsan, Magali Remaud-Simeon, Gabrielle Potocki-Veronese, Claire Moulis
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Publication number: 20180104274Abstract: Polypeptides having the ability to specifically form connections of glucosyl units in alpha 1,3 on an acceptor having at least one hydroxyl moiety are presented. The polypeptides include i) the pattern I of sequence SEQ ID NO: 1, ii) the pattern II of sequence SEQ ID NO: 2, iii) the pattern III of sequence SEQ ID NO: 3, and iv) the pattern IV of sequence SEQ ID NO: 4, or derivates from one or several of said patterns, wherein the polypeptide furthermore has an aspartic residue (D) at position 5 of the pattern II (SEQ ID NO: 2), a glutamic acid residue (E) at position 6 of the pattern III (SEQ ID NO: 3) and an aspartic acid residue (D) at position 6 of the pattern IV (SEQ ID NO: 4). Methods for producing acceptors connected to glucosyl units in alpha 1,3 using the polypeptides are also provided.Type: ApplicationFiled: September 22, 2017Publication date: April 19, 2018Inventors: Magali REMAUD-SIMEON, Marlene VUILLEMIN, Claire MOULIS, Pierre MONSAN, Sandrine MOREL
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Publication number: 20170247669Abstract: The invention relates to: a protein having dextran-saccharase activity and the sequence SEQ ID NO: 1 for an no acid sequence; a protein having dextran-saccharase activity and the sequence SEQ ID NO: 2 for an amino acid sequence; a complex containing a substrate and a protein with dextran-saccharase activity a method for synthesizing dextrans; and dextrans. The invention also relates to a method for synthesizing gluco-oligosaccharides and gluco-conjugates and to the resulting products.Type: ApplicationFiled: July 21, 2015Publication date: August 31, 2017Inventors: Marlène Vuillemin, Marion Claverie, Claire Moulis, Magali Remaud-Simeon, Pierre Monsan, Etienne Severac, Catherine Fontagne-Faucher
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Publication number: 20170145120Abstract: The subject matter of the invention is dextrans which have between 95% and 99% of ?-1,6 glucosidic bonds, a weight-average molar mass Mw at least equal to 0.7×109 g.mol?1, and a dispersity index D of between 1.3 and 3. The invention also relates to a dextran saccharase which makes it possible to produce such dextrans, and to a method for producing said dextrans.Type: ApplicationFiled: June 19, 2015Publication date: May 25, 2017Inventors: Marlène Vuillemin, Marion Claverie, Claire Moulis, Magali Remaud-Simeon, Florent Grimaud, Pierre Monsan, Agnès Sabate, Catherine Garnier, Marguerite Dols-Lafargue, Patrick Lucas
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Publication number: 20170107242Abstract: The invention relates to a method for producing derivatives of O-?-glucosylated flavonoid, comprising at least one step of incubating a glucansucrase with a flavonoid and at least one sucrose, the flavonoid being a flavonoid which is monohydroxylated or hydroxylated in a non-vicinal manner on the B cycle. The invention also relates to novel O-?-glucosylated flavonoid derivatives, and to the use thereof.Type: ApplicationFiled: March 24, 2015Publication date: April 20, 2017Applicants: Institut National de la Recherche Agronomique, Institut National des Sciences Appliquees de Toulouse, Centre National de la Recherche ScientifiqueInventors: Sandrine Morel, Isabelle Andre, Yoan Brison, Emmanuelle Cambon, Yannick Malbert, Denis Pompon, Magali Remaud-Simeon, Philippe Urban
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Publication number: 20170101484Abstract: Compositions for improving the health of a subject comprise alpha-(1,2)-branched alpha-(1,6) oligodextrans, preferably with an average molecular weight between about 10 kDa and 70 kDa, between about 10% and 50% alpha-(1,2)-osidic side chains, and having at least partial indigestibility in the subject. Methods for improving the health of a subject comprise administering the composition to a subject in an amount effective to improve gut health, or to prevent or treat a gastrointestinal disorder, a cholesterol-related disorder, diabetes, or obesity. Methods for making oligodextrans having controlled size and controlled degree of branching comprise providing alpha-(1,6) oligodextrans having an average molecular weight between 0.5 and 100 kDa and introducing at least 10% alpha-(1,2)-osidic side chains onto the alpha-(1,6) oligodextrans.Type: ApplicationFiled: October 21, 2016Publication date: April 13, 2017Applicant: TATE & LYLE INGREDIENTS FRANCE SASInventors: THIERRY NAEYE, ALEXANDRA EINERHAND, MICHEL LOPEZ, SUSAN M. POTTER, MAGALI REMAUD-SIMÉON, PIERRE FRÉDÉRIC EMMANUEL MONSAN