Abstract: The present invention relates to a method for controlling process parameters in a bioreactor when producing a target product with a pre-determined critical to quality, CTQ, profile. The method comprises: monitoring (S20) at least one product quality attribute, PQA, for the target product; identifying (S30) trends and/or deviations by comparing the monitored at least one PQA to the CTQ profile of the target product; and controlling (S40) process parameters of the bioreactor based on the identified trends and/or deviations to maintain the target product within the CTQ profile.

Abstract: The present invention relates to a computer implemented method for controlling a fluid coupling network (170), the fluid coupling network (170) being configured to be fluidly couplable to bioreactor (110), a gas source (120), a buffer source (140), a waste port (150), a processing system (160) and a conduit reservoir (208), the fluid coupling network (170) comprising a barrier unit (220) configured to sterilely separate fluid paths within the fluid coupling network (170), the fluid coupling network (170) being controllable to sample the bioreactor (110), the method comprising obtaining (510) a fluid sample from the bioreactor (110), wherein the step of obtaining the fluid sample comprises controlling a flow of fluid from the bioreactor (110) via the conduit reservoir (208) and the fluid coupling network (170) to the waste port (150), to fill the conduit reservoir (208) with the fluid from the bioreactor (110), providing (530) the fluid sample, wherein the step of providing the fluid sample comprises to provid

Abstract: The invention relates to an electrospray emitter (1′; 1″; 1′″), comprising an essentially tubular member (2; 5; 7) having an inlet end and an outlet end. There is provided a spray aperture (4) in the outlet end, having an inner diameter suitable for the generation of electrospray usable in mass spectrometry. At least a surface portion of the emitter comprises a conductive polymer composition that is stable in conditions prevailing during electrospray.